Isolation of a novel leucine-rich repeat receptor-like kinase (OsLRR2) gene from rice and analysis of its relation to abiotic stress responses
Leucine-rich repeat receptor-like kinase protein (LRR-RLK) is involved in a wide range of biological pathways. So far, the function of LRR-RLK in the growth, developmental processes and various external stimuli has still not been clearly elucidated in rice (Oryza sativa L.). To understand the mechan...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-01-01
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| Series: | Biotechnology & Biotechnological Equipment |
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| Online Access: | http://dx.doi.org/10.1080/13102818.2016.1242377 |
| _version_ | 1811299152216195072 |
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| author | Yongrong Liao Changqiong Hu Xuewei Zhang Xufeng Cao Zhengjun Xu Xiaoling Gao Lihua Li Jianqing Zhu Rongjun Chen |
| author_facet | Yongrong Liao Changqiong Hu Xuewei Zhang Xufeng Cao Zhengjun Xu Xiaoling Gao Lihua Li Jianqing Zhu Rongjun Chen |
| author_sort | Yongrong Liao |
| collection | DOAJ |
| description | Leucine-rich repeat receptor-like kinase protein (LRR-RLK) is involved in a wide range of biological pathways. So far, the function of LRR-RLK in the growth, developmental processes and various external stimuli has still not been clearly elucidated in rice (Oryza sativa L.). To understand the mechanism(s) underlying stress response and to discover novel stress-tolerance genes in rice, we analysed a global genome expression profiling of the indica cultivar Pei'ai 64S subjected to cold, drought or heat stresses. Expression profiles were obtained for leaf and panicle tissues at seedling, booting and heading stages from plants under no stress, or cold, drought or heat stress, using the GeneChip Rice Genome Array (Affymetrix) representing 51279 transcripts from japonica and indica rice. We identified a gene, OsLRR2 (Oryza sativa L. leucine-rich repeat receptor-like kinase 2, GenBank accession: EAZ02952.1), which was highly up-regulated under cold and drought stress. In order to study its function in stress tolerance, we cloned the cDNA of the gene through amplification by reverse-transcription polymerase chain reaction. Sequence analysis showed that the cDNA encodes a protein of 375 amino-acid residues with molecular weight of ≈40.62 kD and pI of ≈5.75. The sequence databases search found that the open reading frame of OsLRR2 contained a leucine-rich repeats domain. Analysis of the putative promoter region for candidate cis-regulatory elements identified five matches to cis-elements related to stress responses, suggesting that OsLRR2 could be considered a new candidate gene involved in stress tolerance in rice. |
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| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-04-13T06:31:04Z |
| publishDate | 2017-01-01 |
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| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-7e0cacff04c74264ad67a12e14be002c2022-12-22T02:58:09ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302017-01-01311515710.1080/13102818.2016.12423771242377Isolation of a novel leucine-rich repeat receptor-like kinase (OsLRR2) gene from rice and analysis of its relation to abiotic stress responsesYongrong Liao0Changqiong Hu1Xuewei Zhang2Xufeng Cao3Zhengjun Xu4Xiaoling Gao5Lihua Li6Jianqing Zhu7Rongjun Chen8Rice Research Institute of Sichuan Agricultural UniversityRice Research Institute of Sichuan Agricultural UniversityRice Research Institute of Sichuan Agricultural UniversityRice Research Institute of Sichuan Agricultural UniversityRice Research Institute of Sichuan Agricultural UniversityRice Research Institute of Sichuan Agricultural UniversityRice Research Institute of Sichuan Agricultural UniversityRice Research Institute of Sichuan Agricultural UniversityRice Research Institute of Sichuan Agricultural UniversityLeucine-rich repeat receptor-like kinase protein (LRR-RLK) is involved in a wide range of biological pathways. So far, the function of LRR-RLK in the growth, developmental processes and various external stimuli has still not been clearly elucidated in rice (Oryza sativa L.). To understand the mechanism(s) underlying stress response and to discover novel stress-tolerance genes in rice, we analysed a global genome expression profiling of the indica cultivar Pei'ai 64S subjected to cold, drought or heat stresses. Expression profiles were obtained for leaf and panicle tissues at seedling, booting and heading stages from plants under no stress, or cold, drought or heat stress, using the GeneChip Rice Genome Array (Affymetrix) representing 51279 transcripts from japonica and indica rice. We identified a gene, OsLRR2 (Oryza sativa L. leucine-rich repeat receptor-like kinase 2, GenBank accession: EAZ02952.1), which was highly up-regulated under cold and drought stress. In order to study its function in stress tolerance, we cloned the cDNA of the gene through amplification by reverse-transcription polymerase chain reaction. Sequence analysis showed that the cDNA encodes a protein of 375 amino-acid residues with molecular weight of ≈40.62 kD and pI of ≈5.75. The sequence databases search found that the open reading frame of OsLRR2 contained a leucine-rich repeats domain. Analysis of the putative promoter region for candidate cis-regulatory elements identified five matches to cis-elements related to stress responses, suggesting that OsLRR2 could be considered a new candidate gene involved in stress tolerance in rice.http://dx.doi.org/10.1080/13102818.2016.1242377Oryza sativa L.abiotic stressmicroarraygene cloningcold and drought stressLRR-RLK |
| spellingShingle | Yongrong Liao Changqiong Hu Xuewei Zhang Xufeng Cao Zhengjun Xu Xiaoling Gao Lihua Li Jianqing Zhu Rongjun Chen Isolation of a novel leucine-rich repeat receptor-like kinase (OsLRR2) gene from rice and analysis of its relation to abiotic stress responses Biotechnology & Biotechnological Equipment Oryza sativa L. abiotic stress microarray gene cloning cold and drought stress LRR-RLK |
| title | Isolation of a novel leucine-rich repeat receptor-like kinase (OsLRR2) gene from rice and analysis of its relation to abiotic stress responses |
| title_full | Isolation of a novel leucine-rich repeat receptor-like kinase (OsLRR2) gene from rice and analysis of its relation to abiotic stress responses |
| title_fullStr | Isolation of a novel leucine-rich repeat receptor-like kinase (OsLRR2) gene from rice and analysis of its relation to abiotic stress responses |
| title_full_unstemmed | Isolation of a novel leucine-rich repeat receptor-like kinase (OsLRR2) gene from rice and analysis of its relation to abiotic stress responses |
| title_short | Isolation of a novel leucine-rich repeat receptor-like kinase (OsLRR2) gene from rice and analysis of its relation to abiotic stress responses |
| title_sort | isolation of a novel leucine rich repeat receptor like kinase oslrr2 gene from rice and analysis of its relation to abiotic stress responses |
| topic | Oryza sativa L. abiotic stress microarray gene cloning cold and drought stress LRR-RLK |
| url | http://dx.doi.org/10.1080/13102818.2016.1242377 |
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