Mesoporous silica-coated silver nanoparticles as ciprofloxacin/siRNA carriers for accelerated infected wound healing
Abstract The colonization of bacterial pathogens is a major concern in wound infection and becoming a public health issue. Herein, a core–shell structured Ag@MSN (silver core embedded with mesoporous silica, AM)-based nanoplatform was elaborately fabricated to co-load ciprofloxacin (CFL) and tumor n...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-08-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-022-01600-9 |
| _version_ | 1811285874958139392 |
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| author | Qiqi Liu Ying Zhang Jingkai Huang Zhourui Xu Xiang Li Jingyu Yang Haoqiang Huang Shiqi Tang Yujuan Chai Jinbo Lin Chengbin Yang Jia Liu Suxia Lin |
| author_facet | Qiqi Liu Ying Zhang Jingkai Huang Zhourui Xu Xiang Li Jingyu Yang Haoqiang Huang Shiqi Tang Yujuan Chai Jinbo Lin Chengbin Yang Jia Liu Suxia Lin |
| author_sort | Qiqi Liu |
| collection | DOAJ |
| description | Abstract The colonization of bacterial pathogens is a major concern in wound infection and becoming a public health issue. Herein, a core–shell structured Ag@MSN (silver core embedded with mesoporous silica, AM)-based nanoplatform was elaborately fabricated to co-load ciprofloxacin (CFL) and tumor necrosis factor-α (TNF-α) small interfering RNA (siTNF-α) (AMPC@siTNF-α) for treating the bacterial-infected wound. The growth of bacterial pathogens was mostly inhibited by released silver ions (Ag+) and CFL from AMPC@siTNF-α. Meanwhile, the loaded siTNF-α was internalized by macrophage cells, which silenced the expression of TNF-α (a pro-inflammatory cytokine) in macrophage cells and accelerated the wound healing process by reducing inflammation response. In the in vivo wound model, the Escherichia coli (E. coli)-infected wound in mice almost completely disappeared after treatment with AMPC@siTNF-α, and no suppuration symptom was observed during the course of the treatment. Importantly, this nanoplatform had negligible side effects both in vitro and in vivo. Taken together, this study strongly demonstrates the promising potential of AMPC@siTNF-α as a synergistic therapeutic agent for clinical wound infections. Graphical Abstract |
| first_indexed | 2024-04-13T02:50:36Z |
| format | Article |
| id | doaj.art-7e0d5e39f4c94427a010e2c409714bed |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-13T02:50:36Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-7e0d5e39f4c94427a010e2c409714bed2022-12-22T03:05:51ZengBMCJournal of Nanobiotechnology1477-31552022-08-0120111610.1186/s12951-022-01600-9Mesoporous silica-coated silver nanoparticles as ciprofloxacin/siRNA carriers for accelerated infected wound healingQiqi Liu0Ying Zhang1Jingkai Huang2Zhourui Xu3Xiang Li4Jingyu Yang5Haoqiang Huang6Shiqi Tang7Yujuan Chai8Jinbo Lin9Chengbin Yang10Jia Liu11Suxia Lin12Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Health Science Center, Shenzhen UniversityGuangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Health Science Center, Shenzhen UniversityDermatology Department, Southern University of Science and Technology Hospital (SUSTech Hospital)Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Health Science Center, Shenzhen UniversityGuangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Health Science Center, Shenzhen UniversityGuangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Health Science Center, Shenzhen UniversityGuangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Health Science Center, Shenzhen UniversityGuangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Health Science Center, Shenzhen UniversityGuangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Health Science Center, Shenzhen UniversityCentral Laboratory, The Second Affiliated Hospital, School of Medicine, Longgang District People’s Hospital of Shenzhen, The Chinese University of Hong KongGuangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Health Science Center, Shenzhen UniversityCentral Laboratory, The Second Affiliated Hospital, School of Medicine, Longgang District People’s Hospital of Shenzhen, The Chinese University of Hong KongCenter of Assisted Reproduction and Embryology, The University of Hong Kong-Shenzhen HospitalAbstract The colonization of bacterial pathogens is a major concern in wound infection and becoming a public health issue. Herein, a core–shell structured Ag@MSN (silver core embedded with mesoporous silica, AM)-based nanoplatform was elaborately fabricated to co-load ciprofloxacin (CFL) and tumor necrosis factor-α (TNF-α) small interfering RNA (siTNF-α) (AMPC@siTNF-α) for treating the bacterial-infected wound. The growth of bacterial pathogens was mostly inhibited by released silver ions (Ag+) and CFL from AMPC@siTNF-α. Meanwhile, the loaded siTNF-α was internalized by macrophage cells, which silenced the expression of TNF-α (a pro-inflammatory cytokine) in macrophage cells and accelerated the wound healing process by reducing inflammation response. In the in vivo wound model, the Escherichia coli (E. coli)-infected wound in mice almost completely disappeared after treatment with AMPC@siTNF-α, and no suppuration symptom was observed during the course of the treatment. Importantly, this nanoplatform had negligible side effects both in vitro and in vivo. Taken together, this study strongly demonstrates the promising potential of AMPC@siTNF-α as a synergistic therapeutic agent for clinical wound infections. Graphical Abstracthttps://doi.org/10.1186/s12951-022-01600-9Mesoporous silicasiRNASynergistic therapyAntibacterialSkin infectionWound healing |
| spellingShingle | Qiqi Liu Ying Zhang Jingkai Huang Zhourui Xu Xiang Li Jingyu Yang Haoqiang Huang Shiqi Tang Yujuan Chai Jinbo Lin Chengbin Yang Jia Liu Suxia Lin Mesoporous silica-coated silver nanoparticles as ciprofloxacin/siRNA carriers for accelerated infected wound healing Journal of Nanobiotechnology Mesoporous silica siRNA Synergistic therapy Antibacterial Skin infection Wound healing |
| title | Mesoporous silica-coated silver nanoparticles as ciprofloxacin/siRNA carriers for accelerated infected wound healing |
| title_full | Mesoporous silica-coated silver nanoparticles as ciprofloxacin/siRNA carriers for accelerated infected wound healing |
| title_fullStr | Mesoporous silica-coated silver nanoparticles as ciprofloxacin/siRNA carriers for accelerated infected wound healing |
| title_full_unstemmed | Mesoporous silica-coated silver nanoparticles as ciprofloxacin/siRNA carriers for accelerated infected wound healing |
| title_short | Mesoporous silica-coated silver nanoparticles as ciprofloxacin/siRNA carriers for accelerated infected wound healing |
| title_sort | mesoporous silica coated silver nanoparticles as ciprofloxacin sirna carriers for accelerated infected wound healing |
| topic | Mesoporous silica siRNA Synergistic therapy Antibacterial Skin infection Wound healing |
| url | https://doi.org/10.1186/s12951-022-01600-9 |
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