Vulgarin, a Sesquiterpene Lactone from <i>Artemisia judaica</i>, Improves the Antidiabetic Effectiveness of Glibenclamide in Streptozotocin-Induced Diabetic Rats via Modulation of PEPCK and G6Pase Genes Expression
The current investigation assessed the effect of the eudesmanolid, Vulgarin (VGN), obtained from <i>Artemisia judaica</i> (<i>A. judaica</i>), on the antidiabetic potential of glibenclamide (GLB) using streptozotocin (STZ) to induce diabetes. Seven groups of rats were used in...
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| author | Hassan N. Althurwi Gamal A. Soliman Rehab F. Abdel-Rahman Reham M. Abd-Elsalam Hanan A. Ogaly Mohammed H. Alqarni Faisal F. Albaqami Maged S. Abdel-Kader |
| author_facet | Hassan N. Althurwi Gamal A. Soliman Rehab F. Abdel-Rahman Reham M. Abd-Elsalam Hanan A. Ogaly Mohammed H. Alqarni Faisal F. Albaqami Maged S. Abdel-Kader |
| author_sort | Hassan N. Althurwi |
| collection | DOAJ |
| description | The current investigation assessed the effect of the eudesmanolid, Vulgarin (VGN), obtained from <i>Artemisia judaica</i> (<i>A. judaica</i>), on the antidiabetic potential of glibenclamide (GLB) using streptozotocin (STZ) to induce diabetes. Seven groups of rats were used in the study; the first group received the vehicle and served as normal control. The diabetic rats of the second to the fifth groups were treated with the vehicle (negative control), GLB at 5 mg/kg (positive control), VGN at 10 mg/kg (VGN-10) and VGN at 20 mg/kg (VGN-20), respectively. The diabetic rats of the sixth and seventh groups were administered combinations of GLB plus VGN-10 and GLB plus VGN-20, respectively. The diabetic rats treated with GLB plus VGN-20 combination showed marked improvement in the fasting blood glucose (FBG), insulin and glycated hemoglobin (HbA1c), as well as the lipid profile, compared with those treated with GLB alone. Further, the pancreatic tissues of the diabetic rats that received the GLB+VGN-20 combination showed superior improvements in lipid peroxidation and antioxidant parameters than those of GLB monotherapy. The insulin content of the β-cells was restored in all treatments, while the levels of glucagon and somatostatin of the α- and δ-endocrine cells were reduced in the pancreatic islets. In addition, the concurrent administration of GLB+VGN-20 was the most effective in restoring PEPCK and G6Pase mRNA expression in the liver. In conclusion, the results demonstrated that the GLB+VGN-20 combination led to greater glycemic improvement in diabetic rats compared with GLB monotherapy through its antioxidant effect and capability to modulate PEPCK and G6Pase gene expression in their livers. |
| first_indexed | 2024-03-09T16:19:47Z |
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| id | doaj.art-7e0e7ebff2244c97b80cd546058c6c62 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T16:19:47Z |
| publishDate | 2022-12-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7e0e7ebff2244c97b80cd546058c6c622023-11-24T15:29:13ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0123241585610.3390/ijms232415856Vulgarin, a Sesquiterpene Lactone from <i>Artemisia judaica</i>, Improves the Antidiabetic Effectiveness of Glibenclamide in Streptozotocin-Induced Diabetic Rats via Modulation of PEPCK and G6Pase Genes ExpressionHassan N. Althurwi0Gamal A. Soliman1Rehab F. Abdel-Rahman2Reham M. Abd-Elsalam3Hanan A. Ogaly4Mohammed H. Alqarni5Faisal F. Albaqami6Maged S. Abdel-Kader7Department of Pharmacology, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi ArabiaDepartment of Pharmacology, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi ArabiaDepartment of Pharmacology, National Research Centre, Giza 12622, EgyptDepartment of Pathology, College of Veterinary Medicine, Cairo University, Giza 12613, EgyptDepartment of Chemistry, College of Science, King Khalid University, Abha 61421, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi ArabiaDepartment of Pharmacology, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi ArabiaThe current investigation assessed the effect of the eudesmanolid, Vulgarin (VGN), obtained from <i>Artemisia judaica</i> (<i>A. judaica</i>), on the antidiabetic potential of glibenclamide (GLB) using streptozotocin (STZ) to induce diabetes. Seven groups of rats were used in the study; the first group received the vehicle and served as normal control. The diabetic rats of the second to the fifth groups were treated with the vehicle (negative control), GLB at 5 mg/kg (positive control), VGN at 10 mg/kg (VGN-10) and VGN at 20 mg/kg (VGN-20), respectively. The diabetic rats of the sixth and seventh groups were administered combinations of GLB plus VGN-10 and GLB plus VGN-20, respectively. The diabetic rats treated with GLB plus VGN-20 combination showed marked improvement in the fasting blood glucose (FBG), insulin and glycated hemoglobin (HbA1c), as well as the lipid profile, compared with those treated with GLB alone. Further, the pancreatic tissues of the diabetic rats that received the GLB+VGN-20 combination showed superior improvements in lipid peroxidation and antioxidant parameters than those of GLB monotherapy. The insulin content of the β-cells was restored in all treatments, while the levels of glucagon and somatostatin of the α- and δ-endocrine cells were reduced in the pancreatic islets. In addition, the concurrent administration of GLB+VGN-20 was the most effective in restoring PEPCK and G6Pase mRNA expression in the liver. In conclusion, the results demonstrated that the GLB+VGN-20 combination led to greater glycemic improvement in diabetic rats compared with GLB monotherapy through its antioxidant effect and capability to modulate PEPCK and G6Pase gene expression in their livers.https://www.mdpi.com/1422-0067/23/24/15856vulgarinstreptozotocinglibenclamidePEPCKG6Pasrat |
| spellingShingle | Hassan N. Althurwi Gamal A. Soliman Rehab F. Abdel-Rahman Reham M. Abd-Elsalam Hanan A. Ogaly Mohammed H. Alqarni Faisal F. Albaqami Maged S. Abdel-Kader Vulgarin, a Sesquiterpene Lactone from <i>Artemisia judaica</i>, Improves the Antidiabetic Effectiveness of Glibenclamide in Streptozotocin-Induced Diabetic Rats via Modulation of PEPCK and G6Pase Genes Expression International Journal of Molecular Sciences vulgarin streptozotocin glibenclamide PEPCK G6Pas rat |
| title | Vulgarin, a Sesquiterpene Lactone from <i>Artemisia judaica</i>, Improves the Antidiabetic Effectiveness of Glibenclamide in Streptozotocin-Induced Diabetic Rats via Modulation of PEPCK and G6Pase Genes Expression |
| title_full | Vulgarin, a Sesquiterpene Lactone from <i>Artemisia judaica</i>, Improves the Antidiabetic Effectiveness of Glibenclamide in Streptozotocin-Induced Diabetic Rats via Modulation of PEPCK and G6Pase Genes Expression |
| title_fullStr | Vulgarin, a Sesquiterpene Lactone from <i>Artemisia judaica</i>, Improves the Antidiabetic Effectiveness of Glibenclamide in Streptozotocin-Induced Diabetic Rats via Modulation of PEPCK and G6Pase Genes Expression |
| title_full_unstemmed | Vulgarin, a Sesquiterpene Lactone from <i>Artemisia judaica</i>, Improves the Antidiabetic Effectiveness of Glibenclamide in Streptozotocin-Induced Diabetic Rats via Modulation of PEPCK and G6Pase Genes Expression |
| title_short | Vulgarin, a Sesquiterpene Lactone from <i>Artemisia judaica</i>, Improves the Antidiabetic Effectiveness of Glibenclamide in Streptozotocin-Induced Diabetic Rats via Modulation of PEPCK and G6Pase Genes Expression |
| title_sort | vulgarin a sesquiterpene lactone from i artemisia judaica i improves the antidiabetic effectiveness of glibenclamide in streptozotocin induced diabetic rats via modulation of pepck and g6pase genes expression |
| topic | vulgarin streptozotocin glibenclamide PEPCK G6Pas rat |
| url | https://www.mdpi.com/1422-0067/23/24/15856 |
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