Female-Biased Expression of <i>R-spondin 1</i> in Chicken Embryonic Gonads Is Estrogen-Dependent
The mechanism of sex determination in chickens, especially the molecular mechanism of female ovarian development, has not yet been fully elucidated. Previous studies have shown that <i>RSPO1</i>, which is associated with ovarian development in mammals, might have a conserved role in chic...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/13/2240 |
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| author | Mingde Zheng Xikui Liu Yu Meng Xiao Lin Jiahui Li Jianguo Zhu Minmeng Zhao Long Liu Tuoyu Geng Daoqing Gong Jun Zhang |
| author_facet | Mingde Zheng Xikui Liu Yu Meng Xiao Lin Jiahui Li Jianguo Zhu Minmeng Zhao Long Liu Tuoyu Geng Daoqing Gong Jun Zhang |
| author_sort | Mingde Zheng |
| collection | DOAJ |
| description | The mechanism of sex determination in chickens, especially the molecular mechanism of female ovarian development, has not yet been fully elucidated. Previous studies have shown that <i>RSPO1</i>, which is associated with ovarian development in mammals, might have a conserved role in chickens. In this study, we systematically investigated the spatiotemporal expression pattern of <i>RSPO1</i> in various tissues, especially gonads, of male and female chicken embryos using qPCR and Western blotting, and we explored its correlation with the expression of key genes in the estrogen pathway using drug treatment or gene overexpression in vivo and in vitro. Our results reveal that <i>RSPO1</i> was widely expressed in all examined tissues of chicken embryos, showing a female bias in gonadal tissues at both the mRNA and protein levels. Surprisingly, <i>RSPO1</i> was not differentially expressed between male and female gonadal cells with fadrozole-induced estrogen pathway blockades, and furthermore, estradiol-induced estrogen stimulation altered the expression of <i>RSPO1</i>. In addition, overexpression of <i>RSPO1</i> in gonadal cells induced the mRNA expression of its downstream target genes, <i>Wnt family member 4</i> (<i>WNT4</i>) and <i>Catenin beta 1</i> (<i>CTNNB1</i>), and that of <i>estrogen receptor α</i> (<i>ERα</i>), an estrogen pathway gene. In summary, this study provided new evidence for elucidating the role of <i>RSPO1</i> in ovarian development in poultry. |
| first_indexed | 2024-03-11T01:48:10Z |
| format | Article |
| id | doaj.art-7e0ea82c9b674ef5b8beaef6e5d65f69 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T01:48:10Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-7e0ea82c9b674ef5b8beaef6e5d65f692023-11-18T16:05:02ZengMDPI AGAnimals2076-26152023-07-011313224010.3390/ani13132240Female-Biased Expression of <i>R-spondin 1</i> in Chicken Embryonic Gonads Is Estrogen-DependentMingde Zheng0Xikui Liu1Yu Meng2Xiao Lin3Jiahui Li4Jianguo Zhu5Minmeng Zhao6Long Liu7Tuoyu Geng8Daoqing Gong9Jun Zhang10College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaThe mechanism of sex determination in chickens, especially the molecular mechanism of female ovarian development, has not yet been fully elucidated. Previous studies have shown that <i>RSPO1</i>, which is associated with ovarian development in mammals, might have a conserved role in chickens. In this study, we systematically investigated the spatiotemporal expression pattern of <i>RSPO1</i> in various tissues, especially gonads, of male and female chicken embryos using qPCR and Western blotting, and we explored its correlation with the expression of key genes in the estrogen pathway using drug treatment or gene overexpression in vivo and in vitro. Our results reveal that <i>RSPO1</i> was widely expressed in all examined tissues of chicken embryos, showing a female bias in gonadal tissues at both the mRNA and protein levels. Surprisingly, <i>RSPO1</i> was not differentially expressed between male and female gonadal cells with fadrozole-induced estrogen pathway blockades, and furthermore, estradiol-induced estrogen stimulation altered the expression of <i>RSPO1</i>. In addition, overexpression of <i>RSPO1</i> in gonadal cells induced the mRNA expression of its downstream target genes, <i>Wnt family member 4</i> (<i>WNT4</i>) and <i>Catenin beta 1</i> (<i>CTNNB1</i>), and that of <i>estrogen receptor α</i> (<i>ERα</i>), an estrogen pathway gene. In summary, this study provided new evidence for elucidating the role of <i>RSPO1</i> in ovarian development in poultry.https://www.mdpi.com/2076-2615/13/13/2240chicken embryo<i>RSPO1</i>ovarygonadal development |
| spellingShingle | Mingde Zheng Xikui Liu Yu Meng Xiao Lin Jiahui Li Jianguo Zhu Minmeng Zhao Long Liu Tuoyu Geng Daoqing Gong Jun Zhang Female-Biased Expression of <i>R-spondin 1</i> in Chicken Embryonic Gonads Is Estrogen-Dependent Animals chicken embryo <i>RSPO1</i> ovary gonadal development |
| title | Female-Biased Expression of <i>R-spondin 1</i> in Chicken Embryonic Gonads Is Estrogen-Dependent |
| title_full | Female-Biased Expression of <i>R-spondin 1</i> in Chicken Embryonic Gonads Is Estrogen-Dependent |
| title_fullStr | Female-Biased Expression of <i>R-spondin 1</i> in Chicken Embryonic Gonads Is Estrogen-Dependent |
| title_full_unstemmed | Female-Biased Expression of <i>R-spondin 1</i> in Chicken Embryonic Gonads Is Estrogen-Dependent |
| title_short | Female-Biased Expression of <i>R-spondin 1</i> in Chicken Embryonic Gonads Is Estrogen-Dependent |
| title_sort | female biased expression of i r spondin 1 i in chicken embryonic gonads is estrogen dependent |
| topic | chicken embryo <i>RSPO1</i> ovary gonadal development |
| url | https://www.mdpi.com/2076-2615/13/13/2240 |
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