Inactivation of Scrapie Prions by the Electrically Charged Disinfectant CAC-717
Previous studies have revealed that the electrically charged disinfectant CAC-717 has strong virucidal and bactericidal effects but is safe for humans and animals. In this study, CAC-717 was further evaluated for its potential effects as a disinfectant against scrapie prions. Western blotting showed...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-07-01
|
| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/9/7/536 |
| _version_ | 1797563365847990272 |
|---|---|
| author | Akikazu Sakudo Yoshifumi Iwamaru Koichi Furusaki Makoto Haritani Rumiko Onishi Morikazu Imamura Takashi Yokoyama Yasuhiro Yoshikawa Takashi Onodera |
| author_facet | Akikazu Sakudo Yoshifumi Iwamaru Koichi Furusaki Makoto Haritani Rumiko Onishi Morikazu Imamura Takashi Yokoyama Yasuhiro Yoshikawa Takashi Onodera |
| author_sort | Akikazu Sakudo |
| collection | DOAJ |
| description | Previous studies have revealed that the electrically charged disinfectant CAC-717 has strong virucidal and bactericidal effects but is safe for humans and animals. In this study, CAC-717 was further evaluated for its potential effects as a disinfectant against scrapie prions. Western blotting showed that CAC-717 reduced the amount of the abnormal isoform of prion protein (PrP<sup>Sc</sup>) in prion-infected cell (ScN2a) lysates. Furthermore, the reduction of prion transmissibility was confirmed by a mouse bioassay, in which mice injected with scrapie prions pre-treated with CAC-717 survived longer than those injected with untreated scrapie prions. Lastly, to evaluate the seeding activity of ScN2a cell lysates treated with CAC-717, quantitative protein misfolding cyclic amplification (PMCA) was performed directly on ScN2a cell lysates treated with CAC-717, which showed that the median dose of PMCA (PMCA<sub>50</sub>) dropped from log9.95 to log5.20 after CAC-717 treatment, indicating more than a 4 log reduction. This suggests that the seeding activity of PrP<sup>Sc</sup> is decreased by CAC-717. Collectively, these results suggest that CAC-717 has anti-prion activity, reducing both PrP<sup>Sc</sup> conversion activity and prion transmissibility; thus, CAC-717 will be useful as a novel disinfectant in prion diseases. |
| first_indexed | 2024-03-10T18:41:36Z |
| format | Article |
| id | doaj.art-7e126c9a5fdb43f7a70f19975fbd5e95 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T18:41:36Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-7e126c9a5fdb43f7a70f19975fbd5e952023-11-20T05:46:46ZengMDPI AGPathogens2076-08172020-07-019753610.3390/pathogens9070536Inactivation of Scrapie Prions by the Electrically Charged Disinfectant CAC-717Akikazu Sakudo0Yoshifumi Iwamaru1Koichi Furusaki2Makoto Haritani3Rumiko Onishi4Morikazu Imamura5Takashi Yokoyama6Yasuhiro Yoshikawa7Takashi Onodera8School of Veterinary Medicine, Okayama University of Science, Imabari, Ehime 794-8555, JapanNational Institute of Animal Health, Ibaraki 305-1002, JapanMineral Activation Research Institute, Kumamoto 865-0023, JapanResearch Center for Food Safety, University of Tokyo, Tokyo 113-8657, JapanSanta Mineral Co., Ltd., Minato-ku, Tokyo 105-0013, JapanFaculty of Medicine, University of Miyazaki, Kiyotake-cho, Miyazaki 889-1692, JapanNational Institute of Animal Health, Ibaraki 305-1002, JapanSchool of Veterinary Medicine, Okayama University of Science, Imabari, Ehime 794-8555, JapanResearch Center for Food Safety, University of Tokyo, Tokyo 113-8657, JapanPrevious studies have revealed that the electrically charged disinfectant CAC-717 has strong virucidal and bactericidal effects but is safe for humans and animals. In this study, CAC-717 was further evaluated for its potential effects as a disinfectant against scrapie prions. Western blotting showed that CAC-717 reduced the amount of the abnormal isoform of prion protein (PrP<sup>Sc</sup>) in prion-infected cell (ScN2a) lysates. Furthermore, the reduction of prion transmissibility was confirmed by a mouse bioassay, in which mice injected with scrapie prions pre-treated with CAC-717 survived longer than those injected with untreated scrapie prions. Lastly, to evaluate the seeding activity of ScN2a cell lysates treated with CAC-717, quantitative protein misfolding cyclic amplification (PMCA) was performed directly on ScN2a cell lysates treated with CAC-717, which showed that the median dose of PMCA (PMCA<sub>50</sub>) dropped from log9.95 to log5.20 after CAC-717 treatment, indicating more than a 4 log reduction. This suggests that the seeding activity of PrP<sup>Sc</sup> is decreased by CAC-717. Collectively, these results suggest that CAC-717 has anti-prion activity, reducing both PrP<sup>Sc</sup> conversion activity and prion transmissibility; thus, CAC-717 will be useful as a novel disinfectant in prion diseases.https://www.mdpi.com/2076-0817/9/7/536anti-prion compoundcalcium hydrogen carbonatemesoscopic structurescrapie prions |
| spellingShingle | Akikazu Sakudo Yoshifumi Iwamaru Koichi Furusaki Makoto Haritani Rumiko Onishi Morikazu Imamura Takashi Yokoyama Yasuhiro Yoshikawa Takashi Onodera Inactivation of Scrapie Prions by the Electrically Charged Disinfectant CAC-717 Pathogens anti-prion compound calcium hydrogen carbonate mesoscopic structure scrapie prions |
| title | Inactivation of Scrapie Prions by the Electrically Charged Disinfectant CAC-717 |
| title_full | Inactivation of Scrapie Prions by the Electrically Charged Disinfectant CAC-717 |
| title_fullStr | Inactivation of Scrapie Prions by the Electrically Charged Disinfectant CAC-717 |
| title_full_unstemmed | Inactivation of Scrapie Prions by the Electrically Charged Disinfectant CAC-717 |
| title_short | Inactivation of Scrapie Prions by the Electrically Charged Disinfectant CAC-717 |
| title_sort | inactivation of scrapie prions by the electrically charged disinfectant cac 717 |
| topic | anti-prion compound calcium hydrogen carbonate mesoscopic structure scrapie prions |
| url | https://www.mdpi.com/2076-0817/9/7/536 |
| work_keys_str_mv | AT akikazusakudo inactivationofscrapieprionsbytheelectricallychargeddisinfectantcac717 AT yoshifumiiwamaru inactivationofscrapieprionsbytheelectricallychargeddisinfectantcac717 AT koichifurusaki inactivationofscrapieprionsbytheelectricallychargeddisinfectantcac717 AT makotoharitani inactivationofscrapieprionsbytheelectricallychargeddisinfectantcac717 AT rumikoonishi inactivationofscrapieprionsbytheelectricallychargeddisinfectantcac717 AT morikazuimamura inactivationofscrapieprionsbytheelectricallychargeddisinfectantcac717 AT takashiyokoyama inactivationofscrapieprionsbytheelectricallychargeddisinfectantcac717 AT yasuhiroyoshikawa inactivationofscrapieprionsbytheelectricallychargeddisinfectantcac717 AT takashionodera inactivationofscrapieprionsbytheelectricallychargeddisinfectantcac717 |