Clinical Biofilm Ring Test<sup>®</sup> Reveals the Potential Role of β-Lactams in the Induction of Biofilm Formation by <i>P. aeruginosa</i> in Cystic Fibrosis Patients
Biofilms are characterized by high tolerance to antimicrobials. However, conventional antibiograms are performed on planktonic microorganisms. Through the clinical Biofilm Ring Test<sup>®</sup> (cBRT), initially aimed to measure the adhesion propensity of bacteria, we discerned a variabl...
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MDPI AG
2020-12-01
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author | Elodie Olivares Jason Tasse Stéphanie Badel-Berchoux Christian Provot Gilles Prévost Thierry Bernardi |
author_facet | Elodie Olivares Jason Tasse Stéphanie Badel-Berchoux Christian Provot Gilles Prévost Thierry Bernardi |
author_sort | Elodie Olivares |
collection | DOAJ |
description | Biofilms are characterized by high tolerance to antimicrobials. However, conventional antibiograms are performed on planktonic microorganisms. Through the clinical Biofilm Ring Test<sup>®</sup> (cBRT), initially aimed to measure the adhesion propensity of bacteria, we discerned a variable distribution of biofilm-producer strains among <i>P. aeruginosa</i> samples isolated from expectorations of cystic fibrosis (CF) patients. Despite a majority of spontaneous adherent isolates, few strains remained planktonic after 5 h of incubation. Their analysis by an adapted protocol of the cBRT revealed an induction of the biofilm early formation by sub-inhibitory doses of β-lactams. Microscopic observations of bacterial cultures stained with Syto 9/Propidium Iodide (PI) confirmed the ability of antimicrobials to increase either the bacterial biomass or the biovolume occupied by induced sessile cells. Finally, the cBRT and its derivatives enabled to highlight in a few hours the potential inducer property of antibiotics on bacterial adhesion. This phenomenon should be considered carefully in the context of CF since patients are constantly under fluctuating antimicrobial treatments. To conclude, assays derived from the Biofilm Ring Test<sup>®</sup> (BRT) device, not only define efficient doses preventing biofilm formation, but could be useful for the antimicrobial selection in CF, to avoid inducer molecules of the early biofilm initiation. |
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language | English |
last_indexed | 2024-03-10T13:56:15Z |
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spelling | doaj.art-7e12d61748c744a9aade09cedd29f3ec2023-11-21T01:37:20ZengMDPI AGPathogens2076-08172020-12-01912106510.3390/pathogens9121065Clinical Biofilm Ring Test<sup>®</sup> Reveals the Potential Role of β-Lactams in the Induction of Biofilm Formation by <i>P. aeruginosa</i> in Cystic Fibrosis PatientsElodie Olivares0Jason Tasse1Stéphanie Badel-Berchoux2Christian Provot3Gilles Prévost4Thierry Bernardi5Virulence Bactérienne Précoce UR7290, ITI Innovec, FMTS, Institut de Bactériologie, Université de Strasbourg, 3 Rue Koeberlé, 67000 Strasbourg, FranceBioFilm Pharma SAS, 317 Avenue Jean Jaurès, 69007 Lyon, FranceBioFilm Control SAS, Rue Emile Duclaux, 63360 Saint Beauzire, FranceBioFilm Pharma SAS, 317 Avenue Jean Jaurès, 69007 Lyon, FranceVirulence Bactérienne Précoce UR7290, ITI Innovec, FMTS, Institut de Bactériologie, Université de Strasbourg, 3 Rue Koeberlé, 67000 Strasbourg, FranceBioFilm Pharma SAS, 317 Avenue Jean Jaurès, 69007 Lyon, FranceBiofilms are characterized by high tolerance to antimicrobials. However, conventional antibiograms are performed on planktonic microorganisms. Through the clinical Biofilm Ring Test<sup>®</sup> (cBRT), initially aimed to measure the adhesion propensity of bacteria, we discerned a variable distribution of biofilm-producer strains among <i>P. aeruginosa</i> samples isolated from expectorations of cystic fibrosis (CF) patients. Despite a majority of spontaneous adherent isolates, few strains remained planktonic after 5 h of incubation. Their analysis by an adapted protocol of the cBRT revealed an induction of the biofilm early formation by sub-inhibitory doses of β-lactams. Microscopic observations of bacterial cultures stained with Syto 9/Propidium Iodide (PI) confirmed the ability of antimicrobials to increase either the bacterial biomass or the biovolume occupied by induced sessile cells. Finally, the cBRT and its derivatives enabled to highlight in a few hours the potential inducer property of antibiotics on bacterial adhesion. This phenomenon should be considered carefully in the context of CF since patients are constantly under fluctuating antimicrobial treatments. To conclude, assays derived from the Biofilm Ring Test<sup>®</sup> (BRT) device, not only define efficient doses preventing biofilm formation, but could be useful for the antimicrobial selection in CF, to avoid inducer molecules of the early biofilm initiation.https://www.mdpi.com/2076-0817/9/12/1065biofilmcystic fibrosis<i>Pseudomonas aeruginosa</i>antibioticsclinical Biofilm Ring Test<sup>®</sup>early bacterial adhesion induction |
spellingShingle | Elodie Olivares Jason Tasse Stéphanie Badel-Berchoux Christian Provot Gilles Prévost Thierry Bernardi Clinical Biofilm Ring Test<sup>®</sup> Reveals the Potential Role of β-Lactams in the Induction of Biofilm Formation by <i>P. aeruginosa</i> in Cystic Fibrosis Patients Pathogens biofilm cystic fibrosis <i>Pseudomonas aeruginosa</i> antibiotics clinical Biofilm Ring Test<sup>®</sup> early bacterial adhesion induction |
title | Clinical Biofilm Ring Test<sup>®</sup> Reveals the Potential Role of β-Lactams in the Induction of Biofilm Formation by <i>P. aeruginosa</i> in Cystic Fibrosis Patients |
title_full | Clinical Biofilm Ring Test<sup>®</sup> Reveals the Potential Role of β-Lactams in the Induction of Biofilm Formation by <i>P. aeruginosa</i> in Cystic Fibrosis Patients |
title_fullStr | Clinical Biofilm Ring Test<sup>®</sup> Reveals the Potential Role of β-Lactams in the Induction of Biofilm Formation by <i>P. aeruginosa</i> in Cystic Fibrosis Patients |
title_full_unstemmed | Clinical Biofilm Ring Test<sup>®</sup> Reveals the Potential Role of β-Lactams in the Induction of Biofilm Formation by <i>P. aeruginosa</i> in Cystic Fibrosis Patients |
title_short | Clinical Biofilm Ring Test<sup>®</sup> Reveals the Potential Role of β-Lactams in the Induction of Biofilm Formation by <i>P. aeruginosa</i> in Cystic Fibrosis Patients |
title_sort | clinical biofilm ring test sup r sup reveals the potential role of β lactams in the induction of biofilm formation by i p aeruginosa i in cystic fibrosis patients |
topic | biofilm cystic fibrosis <i>Pseudomonas aeruginosa</i> antibiotics clinical Biofilm Ring Test<sup>®</sup> early bacterial adhesion induction |
url | https://www.mdpi.com/2076-0817/9/12/1065 |
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