Serovars, antimicrobial susceptibility and molecular characteristics of haemophilus parasuis isolates in southern china
This study analyzed the characteristics of 133 Haemophilus parasuis isolates in southern China. These isolates belonged to eleven serovars (1, 2, 4-10, 13 and 15) with 12.0% of them being characterised as non-typable. A relatively high level in resistance was encountered for trimethoprim + sulfameth...
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Kafkas University, Faculty of Veterinary Medicine
2020-03-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=2692 |
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| author | Ling PENG Xiaoqing YUAN Ran FANG Weizhen LIU-FU Quan WEN Xufu YANG |
| author_facet | Ling PENG Xiaoqing YUAN Ran FANG Weizhen LIU-FU Quan WEN Xufu YANG |
| author_sort | Ling PENG |
| collection | DOAJ |
| description | This study analyzed the characteristics of 133 Haemophilus parasuis isolates in southern China. These isolates belonged to eleven serovars
(1, 2, 4-10, 13 and 15) with 12.0% of them being characterised as non-typable. A relatively high level in resistance was encountered for
trimethoprim + sulfamethoxazole (89.9%), tetracycline (75.3%), amoxicillin (69.1%), streptomycin (63.6%), carbenicillin (60.2%), kanamycin
(46.6%) and ampicillin (45.6%). A total of 60% of the isolates were negative for group 1 virulence-associated autotransporters (vtaA). All group
1 vtaA negative isolates fell into polyacrylamide gel electrophoresis (PAGE) type I, while all group 1 vtaA positive isolates were classified as
PAGE type II. The results of Multi-locus sequence typing (MLST) indicated a high degree of variation, 45 isolates in the study were assigned
into 31 sequence types with 28 of these being new (not found in the MLST database). Antimicrobial resistance was observed in every serovar,
there was no statistically significant correlation between the antimicrobial resistance and the serovars. The isolates allocated to clade 2
(based on MLST target sequences) showed the molecular characteristics of highly pathogenic strains in whole-cell protein profiling, vtaA
groups 1, superoxide dismutase (sodA) sequence and MLST. |
| first_indexed | 2024-03-13T04:39:26Z |
| format | Article |
| id | doaj.art-7e180d0c841b4bc98076403407dfa2db |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:39:26Z |
| publishDate | 2020-03-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-7e180d0c841b4bc98076403407dfa2db2023-06-19T06:32:43ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512020-03-0126449149710.9775/kvfd.2019.235872692Serovars, antimicrobial susceptibility and molecular characteristics of haemophilus parasuis isolates in southern chinaLing PENG0Xiaoqing YUAN1Ran FANG2Weizhen LIU-FU3Quan WEN4Xufu YANG5Yingdong College of Biology and Agriculture, Shaoguan University, Shaoguan 512005, CHINAYingdong College of Biology and Agriculture, Shaoguan University, Shaoguan 512005, CHINAYingdong College of Biology and Agriculture, Shaoguan University, Shaoguan 512005, CHINAYingdong College of Biology and Agriculture, Shaoguan University, Shaoguan 512005, CHINAYingdong College of Biology and Agriculture, Shaoguan University, Shaoguan 512005, CHINAYingdong College of Biology and Agriculture, Shaoguan University, Shaoguan 512005, CHINAThis study analyzed the characteristics of 133 Haemophilus parasuis isolates in southern China. These isolates belonged to eleven serovars (1, 2, 4-10, 13 and 15) with 12.0% of them being characterised as non-typable. A relatively high level in resistance was encountered for trimethoprim + sulfamethoxazole (89.9%), tetracycline (75.3%), amoxicillin (69.1%), streptomycin (63.6%), carbenicillin (60.2%), kanamycin (46.6%) and ampicillin (45.6%). A total of 60% of the isolates were negative for group 1 virulence-associated autotransporters (vtaA). All group 1 vtaA negative isolates fell into polyacrylamide gel electrophoresis (PAGE) type I, while all group 1 vtaA positive isolates were classified as PAGE type II. The results of Multi-locus sequence typing (MLST) indicated a high degree of variation, 45 isolates in the study were assigned into 31 sequence types with 28 of these being new (not found in the MLST database). Antimicrobial resistance was observed in every serovar, there was no statistically significant correlation between the antimicrobial resistance and the serovars. The isolates allocated to clade 2 (based on MLST target sequences) showed the molecular characteristics of highly pathogenic strains in whole-cell protein profiling, vtaA groups 1, superoxide dismutase (sodA) sequence and MLST.https://vetdergikafkas.org/pdf.php?id=2692haemophilus parasuisantimicrobial susceptibilitymlstpagevtaasoda |
| spellingShingle | Ling PENG Xiaoqing YUAN Ran FANG Weizhen LIU-FU Quan WEN Xufu YANG Serovars, antimicrobial susceptibility and molecular characteristics of haemophilus parasuis isolates in southern china Kafkas Universitesi Veteriner Fakültesi Dergisi haemophilus parasuis antimicrobial susceptibility mlst page vtaa soda |
| title | Serovars, antimicrobial susceptibility and molecular characteristics of haemophilus parasuis isolates in southern china |
| title_full | Serovars, antimicrobial susceptibility and molecular characteristics of haemophilus parasuis isolates in southern china |
| title_fullStr | Serovars, antimicrobial susceptibility and molecular characteristics of haemophilus parasuis isolates in southern china |
| title_full_unstemmed | Serovars, antimicrobial susceptibility and molecular characteristics of haemophilus parasuis isolates in southern china |
| title_short | Serovars, antimicrobial susceptibility and molecular characteristics of haemophilus parasuis isolates in southern china |
| title_sort | serovars antimicrobial susceptibility and molecular characteristics of haemophilus parasuis isolates in southern china |
| topic | haemophilus parasuis antimicrobial susceptibility mlst page vtaa soda |
| url | https://vetdergikafkas.org/pdf.php?id=2692 |
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