Evaluating a potential model to analyze the function of the gut microbiota of the giant panda
To contribute to the conservation of endangered animals, the utilization of model systems is critical to analyze the function of their gut microbiota. In this study, the results of a fecal microbial transplantation (FMT) experiment with germ-free (GF) mice receiving giant panda or horse fecal microb...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1086058/full |
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| author | Wenping Zhang Wenping Zhang Junjin Xie Junjin Xie Junjin Xie Shan Xia Xueyang Fan Xueyang Fan Stephan Schmitz-Esser Benhua Zeng Lijun Zheng Lijun Zheng He Huang He Huang Hairui Wang Hairui Wang Jincheng Zhong Zhihe Zhang Liang Zhang Liang Zhang Mingfeng Jiang Rong Hou Rong Hou |
| author_facet | Wenping Zhang Wenping Zhang Junjin Xie Junjin Xie Junjin Xie Shan Xia Xueyang Fan Xueyang Fan Stephan Schmitz-Esser Benhua Zeng Lijun Zheng Lijun Zheng He Huang He Huang Hairui Wang Hairui Wang Jincheng Zhong Zhihe Zhang Liang Zhang Liang Zhang Mingfeng Jiang Rong Hou Rong Hou |
| author_sort | Wenping Zhang |
| collection | DOAJ |
| description | To contribute to the conservation of endangered animals, the utilization of model systems is critical to analyze the function of their gut microbiota. In this study, the results of a fecal microbial transplantation (FMT) experiment with germ-free (GF) mice receiving giant panda or horse fecal microbiota showed a clear clustering by donor microbial communities in GF mice, which was consistent with the results of blood metabolites from these mice. At the genus level, FMT re-established approximately 9% of the giant panda donor microbiota in GF mice compared to about 32% for the horse donor microbiota. In line with this, the difference between the panda donor microbiota and panda-mice microbiota on whole-community level was significantly larger than that between the horse donor microbiota and the horse-mice microbiota. These results were consistent with source tracking analysis that found a significantly higher retention rate of the horse donor microbiota (30.9%) than the giant panda donor microbiota (4.0%) in GF mice where the microbiota remained stable after FMT. Further analyzes indicated that the possible reason for the low retention rate of the panda donor microbiota in GF mice was a low relative abundance of Clostridiaceae in the panda donor microbiota. Our results indicate that the donor microbiota has a large effect on GF mice microbiota after FMT. |
| first_indexed | 2024-04-13T04:16:43Z |
| format | Article |
| id | doaj.art-7e1a0918cbee406bb9c63d528cf4fce0 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T04:16:43Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-7e1a0918cbee406bb9c63d528cf4fce02022-12-22T03:02:58ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-12-011310.3389/fmicb.2022.10860581086058Evaluating a potential model to analyze the function of the gut microbiota of the giant pandaWenping Zhang0Wenping Zhang1Junjin Xie2Junjin Xie3Junjin Xie4Shan Xia5Xueyang Fan6Xueyang Fan7Stephan Schmitz-Esser8Benhua Zeng9Lijun Zheng10Lijun Zheng11He Huang12He Huang13Hairui Wang14Hairui Wang15Jincheng Zhong16Zhihe Zhang17Liang Zhang18Liang Zhang19Mingfeng Jiang20Rong Hou21Rong Hou22Chengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan, ChinaSichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu, Sichuan, ChinaChengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan, ChinaSichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu, Sichuan, ChinaQinghai-Tibet Plateau Research Institute, Southwest Minzu University, Chengdu, Sichuan, ChinaCollege of Chemistry and Life Science, Chengdu Normal University, Chengdu, Sichuan, ChinaChengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan, ChinaSichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu, Sichuan, ChinaDepartment of Animal Science, Iowa State University, Ames, IA, United StatesDepartment of Infectious Diseases, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing, ChinaChengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan, ChinaSichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu, Sichuan, ChinaChengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan, ChinaSichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu, Sichuan, ChinaChengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan, ChinaSichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu, Sichuan, ChinaQinghai-Tibet Plateau Research Institute, Southwest Minzu University, Chengdu, Sichuan, ChinaSichuan Academy of Giant Panda, Chengdu, Sichuan, ChinaChengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan, ChinaSichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu, Sichuan, ChinaQinghai-Tibet Plateau Research Institute, Southwest Minzu University, Chengdu, Sichuan, ChinaChengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan, ChinaSichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu, Sichuan, ChinaTo contribute to the conservation of endangered animals, the utilization of model systems is critical to analyze the function of their gut microbiota. In this study, the results of a fecal microbial transplantation (FMT) experiment with germ-free (GF) mice receiving giant panda or horse fecal microbiota showed a clear clustering by donor microbial communities in GF mice, which was consistent with the results of blood metabolites from these mice. At the genus level, FMT re-established approximately 9% of the giant panda donor microbiota in GF mice compared to about 32% for the horse donor microbiota. In line with this, the difference between the panda donor microbiota and panda-mice microbiota on whole-community level was significantly larger than that between the horse donor microbiota and the horse-mice microbiota. These results were consistent with source tracking analysis that found a significantly higher retention rate of the horse donor microbiota (30.9%) than the giant panda donor microbiota (4.0%) in GF mice where the microbiota remained stable after FMT. Further analyzes indicated that the possible reason for the low retention rate of the panda donor microbiota in GF mice was a low relative abundance of Clostridiaceae in the panda donor microbiota. Our results indicate that the donor microbiota has a large effect on GF mice microbiota after FMT.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1086058/fullgiant pandahorsegerm-free mice16S rRNA gene sequencingblood metabolites |
| spellingShingle | Wenping Zhang Wenping Zhang Junjin Xie Junjin Xie Junjin Xie Shan Xia Xueyang Fan Xueyang Fan Stephan Schmitz-Esser Benhua Zeng Lijun Zheng Lijun Zheng He Huang He Huang Hairui Wang Hairui Wang Jincheng Zhong Zhihe Zhang Liang Zhang Liang Zhang Mingfeng Jiang Rong Hou Rong Hou Evaluating a potential model to analyze the function of the gut microbiota of the giant panda Frontiers in Microbiology giant panda horse germ-free mice 16S rRNA gene sequencing blood metabolites |
| title | Evaluating a potential model to analyze the function of the gut microbiota of the giant panda |
| title_full | Evaluating a potential model to analyze the function of the gut microbiota of the giant panda |
| title_fullStr | Evaluating a potential model to analyze the function of the gut microbiota of the giant panda |
| title_full_unstemmed | Evaluating a potential model to analyze the function of the gut microbiota of the giant panda |
| title_short | Evaluating a potential model to analyze the function of the gut microbiota of the giant panda |
| title_sort | evaluating a potential model to analyze the function of the gut microbiota of the giant panda |
| topic | giant panda horse germ-free mice 16S rRNA gene sequencing blood metabolites |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1086058/full |
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