Therapeutic ultrasound treatment for the prevention of chronic kidney disease-associated muscle wasting in mice
AbstractLow-intensity pulsed ultrasound (LIPUS) is a kind of therapeutic ultrasound. It can help improve bone fracture repair and soft tissue healing. Our previous study found that LIPUS treatment could halt the chronic kidney disease (CKD) progression in mice; unexpectedly, we observed the improvem...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2023.2213735 |
| _version_ | 1827765330680741888 |
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| author | Chen-Yu Lin Te-I Weng Jui-Zhi Loh Ding-Cheng Chan Chih-Kang Chiang Kuan-Yu Hung Shing-Hwa Liu |
| author_facet | Chen-Yu Lin Te-I Weng Jui-Zhi Loh Ding-Cheng Chan Chih-Kang Chiang Kuan-Yu Hung Shing-Hwa Liu |
| author_sort | Chen-Yu Lin |
| collection | DOAJ |
| description | AbstractLow-intensity pulsed ultrasound (LIPUS) is a kind of therapeutic ultrasound. It can help improve bone fracture repair and soft tissue healing. Our previous study found that LIPUS treatment could halt the chronic kidney disease (CKD) progression in mice; unexpectedly, we observed the improvement of CKD-reduced muscle weights by LIPUS treatment. Here, we further tested the protective potential of LIPUS on CKD-associated muscle wasting/sarcopenia using the CKD mouse models. Mouse models of both unilateral renal ischemia/reperfusion injury (IRI) with nephrectomy and adenine administration were used to induce CKD. LIPUS with condition of 3 MHz, 100 mW/cm2, 20 min/day was applied to the kidney of CKD mice. LIPUS treatment significantly reversed the increased serum BUN/creatinine levels in CKD mice. LIPUS effectively prevented the decrease in grip strength, muscle weight (soleus, tibialis anterior, and gastrocnemius muscles), cross-section areas of muscle fibres, and muscular phosphorylated Akt protein expression by immunohistochemistry, and the increase in muscular atrogenes Atrogin1 and MuRF1 protein expression by immunohistochemistry in CKD mice. These results indicated that LIPUS could help improve weak muscle strength, muscle mass loss, muscle atrophy-related protein expression, and Akt inactivation. LIPUS application may be an alternative non-invasive therapeutic intervention on the management of CKD-associated muscle wasting. |
| first_indexed | 2024-03-11T11:18:34Z |
| format | Article |
| id | doaj.art-7e1bf771d30a43d78c58978348012cbb |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-03-11T11:18:34Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-7e1bf771d30a43d78c58978348012cbb2023-11-10T17:19:32ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2023-12-0151126827510.1080/21691401.2023.2213735Therapeutic ultrasound treatment for the prevention of chronic kidney disease-associated muscle wasting in miceChen-Yu Lin0Te-I Weng1Jui-Zhi Loh2Ding-Cheng Chan3Chih-Kang Chiang4Kuan-Yu Hung5Shing-Hwa Liu6Institute of Toxicology, College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Forensic Medicine, College of Medicine, National Taiwan University, Taipei, TaiwanInstitute of Toxicology, College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Geriatrics and Gerontology, College of Medicine, National Taiwan University, Taipei, TaiwanInstitute of Toxicology, College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Internal Medicine, College of Medicine, National Taiwan University, Taipei, TaiwanInstitute of Toxicology, College of Medicine, National Taiwan University, Taipei, TaiwanAbstractLow-intensity pulsed ultrasound (LIPUS) is a kind of therapeutic ultrasound. It can help improve bone fracture repair and soft tissue healing. Our previous study found that LIPUS treatment could halt the chronic kidney disease (CKD) progression in mice; unexpectedly, we observed the improvement of CKD-reduced muscle weights by LIPUS treatment. Here, we further tested the protective potential of LIPUS on CKD-associated muscle wasting/sarcopenia using the CKD mouse models. Mouse models of both unilateral renal ischemia/reperfusion injury (IRI) with nephrectomy and adenine administration were used to induce CKD. LIPUS with condition of 3 MHz, 100 mW/cm2, 20 min/day was applied to the kidney of CKD mice. LIPUS treatment significantly reversed the increased serum BUN/creatinine levels in CKD mice. LIPUS effectively prevented the decrease in grip strength, muscle weight (soleus, tibialis anterior, and gastrocnemius muscles), cross-section areas of muscle fibres, and muscular phosphorylated Akt protein expression by immunohistochemistry, and the increase in muscular atrogenes Atrogin1 and MuRF1 protein expression by immunohistochemistry in CKD mice. These results indicated that LIPUS could help improve weak muscle strength, muscle mass loss, muscle atrophy-related protein expression, and Akt inactivation. LIPUS application may be an alternative non-invasive therapeutic intervention on the management of CKD-associated muscle wasting.https://www.tandfonline.com/doi/10.1080/21691401.2023.2213735Low-intensity pulsed ultrasoundchronic kidney diseasemouse modelsmuscle wasting |
| spellingShingle | Chen-Yu Lin Te-I Weng Jui-Zhi Loh Ding-Cheng Chan Chih-Kang Chiang Kuan-Yu Hung Shing-Hwa Liu Therapeutic ultrasound treatment for the prevention of chronic kidney disease-associated muscle wasting in mice Artificial Cells, Nanomedicine, and Biotechnology Low-intensity pulsed ultrasound chronic kidney disease mouse models muscle wasting |
| title | Therapeutic ultrasound treatment for the prevention of chronic kidney disease-associated muscle wasting in mice |
| title_full | Therapeutic ultrasound treatment for the prevention of chronic kidney disease-associated muscle wasting in mice |
| title_fullStr | Therapeutic ultrasound treatment for the prevention of chronic kidney disease-associated muscle wasting in mice |
| title_full_unstemmed | Therapeutic ultrasound treatment for the prevention of chronic kidney disease-associated muscle wasting in mice |
| title_short | Therapeutic ultrasound treatment for the prevention of chronic kidney disease-associated muscle wasting in mice |
| title_sort | therapeutic ultrasound treatment for the prevention of chronic kidney disease associated muscle wasting in mice |
| topic | Low-intensity pulsed ultrasound chronic kidney disease mouse models muscle wasting |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2023.2213735 |
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