Inter-Golgi transport mediated by COPI-containing vesicles carrying small cargoes
A core prediction of the vesicular transport model is that COPI vesicles are responsible for trafficking anterograde cargoes forward. In this study, we test this prediction by examining the properties and requirements of inter-Golgi transport within fused cells, which requires mobile carriers in ord...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2013-10-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/01296 |
| _version_ | 1811180617648308224 |
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| author | Patrina A Pellett Felix Dietrich Jörg Bewersdorf James E Rothman Grégory Lavieu |
| author_facet | Patrina A Pellett Felix Dietrich Jörg Bewersdorf James E Rothman Grégory Lavieu |
| author_sort | Patrina A Pellett |
| collection | DOAJ |
| description | A core prediction of the vesicular transport model is that COPI vesicles are responsible for trafficking anterograde cargoes forward. In this study, we test this prediction by examining the properties and requirements of inter-Golgi transport within fused cells, which requires mobile carriers in order for exchange of constituents to occur. We report that both small soluble and membrane-bound secretory cargo and exogenous Golgi resident glycosyl-transferases are exchanged between separated Golgi. Large soluble aggregates, which traverse individual stacks, do not transfer between Golgi, implying that small cargoes (which can fit in a typical transport vesicle) are transported by a different mechanism. Super-resolution microscopy reveals that the carriers of both anterograde and retrograde cargoes are the size of COPI vesicles, contain coatomer, and functionally require ARF1 and coatomer for transport. The data suggest that COPI vesicles traffic both small secretory cargo and steady-state Golgi resident enzymes among stacked cisternae that are stationary. |
| first_indexed | 2024-04-11T09:06:16Z |
| format | Article |
| id | doaj.art-7e1e42812f2f4fce9655015ce9fdbaa0 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:06:16Z |
| publishDate | 2013-10-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-7e1e42812f2f4fce9655015ce9fdbaa02022-12-22T04:32:37ZengeLife Sciences Publications LtdeLife2050-084X2013-10-01210.7554/eLife.01296Inter-Golgi transport mediated by COPI-containing vesicles carrying small cargoesPatrina A Pellett0Felix Dietrich1Jörg Bewersdorf2James E Rothman3Grégory Lavieu4Department of Cell Biology, Yale University School of Medicine, New Haven, United States; Department of Chemistry, Yale University, New Haven, United StatesDepartment of Cell Biology, Yale University School of Medicine, New Haven, United States; Department of Mathematics, Technische Universität München, Garching, GermanyDepartment of Cell Biology, Yale University School of Medicine, New Haven, United StatesDepartment of Cell Biology, Yale University School of Medicine, New Haven, United StatesDepartment of Cell Biology, Yale University School of Medicine, New Haven, United StatesA core prediction of the vesicular transport model is that COPI vesicles are responsible for trafficking anterograde cargoes forward. In this study, we test this prediction by examining the properties and requirements of inter-Golgi transport within fused cells, which requires mobile carriers in order for exchange of constituents to occur. We report that both small soluble and membrane-bound secretory cargo and exogenous Golgi resident glycosyl-transferases are exchanged between separated Golgi. Large soluble aggregates, which traverse individual stacks, do not transfer between Golgi, implying that small cargoes (which can fit in a typical transport vesicle) are transported by a different mechanism. Super-resolution microscopy reveals that the carriers of both anterograde and retrograde cargoes are the size of COPI vesicles, contain coatomer, and functionally require ARF1 and coatomer for transport. The data suggest that COPI vesicles traffic both small secretory cargo and steady-state Golgi resident enzymes among stacked cisternae that are stationary.https://elifesciences.org/articles/01296Golgimembrane traffickingcoatomer |
| spellingShingle | Patrina A Pellett Felix Dietrich Jörg Bewersdorf James E Rothman Grégory Lavieu Inter-Golgi transport mediated by COPI-containing vesicles carrying small cargoes eLife Golgi membrane trafficking coatomer |
| title | Inter-Golgi transport mediated by COPI-containing vesicles carrying small cargoes |
| title_full | Inter-Golgi transport mediated by COPI-containing vesicles carrying small cargoes |
| title_fullStr | Inter-Golgi transport mediated by COPI-containing vesicles carrying small cargoes |
| title_full_unstemmed | Inter-Golgi transport mediated by COPI-containing vesicles carrying small cargoes |
| title_short | Inter-Golgi transport mediated by COPI-containing vesicles carrying small cargoes |
| title_sort | inter golgi transport mediated by copi containing vesicles carrying small cargoes |
| topic | Golgi membrane trafficking coatomer |
| url | https://elifesciences.org/articles/01296 |
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