Establishment of a human iPSC line (MUSIi007-A) from peripheral blood of normal individual using Sendai viral vectors
Human induced pluripotent stem cell (iPSC) line was generated from peripheral blood mononuclear cells (PBMNCs) isolated from a 26-year-old healthy subject to use as a control group for the iPSC line carrying compound heterozygote for mutation in KLF1 gene. The cells were reprogrammed using integrati...
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| Format: | Article |
| Language: | English |
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Elsevier
2018-10-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S187350611830206X |
| _version_ | 1818765638097698816 |
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| author | Ponthip Potirat Methichit Wattanapanitch Pakpoom Kheolamai Surapol Issaragrisil |
| author_facet | Ponthip Potirat Methichit Wattanapanitch Pakpoom Kheolamai Surapol Issaragrisil |
| author_sort | Ponthip Potirat |
| collection | DOAJ |
| description | Human induced pluripotent stem cell (iPSC) line was generated from peripheral blood mononuclear cells (PBMNCs) isolated from a 26-year-old healthy subject to use as a control group for the iPSC line carrying compound heterozygote for mutation in KLF1 gene. The cells were reprogrammed using integration-free method, Sendai viral (SeV) vectors containing KOS, hc-MYC and hKLF4. The established iPSC line (MUSIi007-A) exhibited a normal karyotype, expressed pluripotent markers and displayed in vitro and in vivo differentiation potential into cells of three embryonic germ layers.Resource tableUnlabelled TableUnique stem cell line identifierMUSIi007-AAlternative name(s) of stem cell lineSeV-iPSCInstitutionSiriraj Center of Excellence for Stem Cell Research,Faculty of Medicine Siriraj Hospital, Mahidol UniversityContact information of distributorPonthip Potirat, ponthip.pot@gmail.comSurapol Issaragrisil, surapolsi@gmail.comType of cell lineiPSCsOriginHumanAdditional origin infoAge: 26-year-oldSex: MaleEthnicity: ThaiCell sourcePeripheral blood mononuclear cellsClonalityClonalMethod of reprogrammingIntegration-free Sendai viral vectorsGenetic modificationN/AType of modificationN/AAssociated diseaseN/AGene/locusN/AMethod of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock dateDecember 2014Cell line repository/bankN/AEthical approvalSiriraj Institutional Review Board (SiRB), no. Si248/2011, Faculty of Medicine Siriraj Hospital, Mahidol University, Thailand. |
| first_indexed | 2024-12-18T08:21:16Z |
| format | Article |
| id | doaj.art-7e1f1db42ac84bfd8cbf43f02e5f5025 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-18T08:21:16Z |
| publishDate | 2018-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-7e1f1db42ac84bfd8cbf43f02e5f50252022-12-21T21:14:44ZengElsevierStem Cell Research1873-50612018-10-01324346Establishment of a human iPSC line (MUSIi007-A) from peripheral blood of normal individual using Sendai viral vectorsPonthip Potirat0Methichit Wattanapanitch1Pakpoom Kheolamai2Surapol Issaragrisil3Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandSiriraj Center for Regenerative Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandDivision of Cell Biology, Department of Pre-clinical Sciences, Faculty of Medicine, Thammasat University, Pathumthani, ThailandSiriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Division of Hematology, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Corresponding author at: Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.Human induced pluripotent stem cell (iPSC) line was generated from peripheral blood mononuclear cells (PBMNCs) isolated from a 26-year-old healthy subject to use as a control group for the iPSC line carrying compound heterozygote for mutation in KLF1 gene. The cells were reprogrammed using integration-free method, Sendai viral (SeV) vectors containing KOS, hc-MYC and hKLF4. The established iPSC line (MUSIi007-A) exhibited a normal karyotype, expressed pluripotent markers and displayed in vitro and in vivo differentiation potential into cells of three embryonic germ layers.Resource tableUnlabelled TableUnique stem cell line identifierMUSIi007-AAlternative name(s) of stem cell lineSeV-iPSCInstitutionSiriraj Center of Excellence for Stem Cell Research,Faculty of Medicine Siriraj Hospital, Mahidol UniversityContact information of distributorPonthip Potirat, ponthip.pot@gmail.comSurapol Issaragrisil, surapolsi@gmail.comType of cell lineiPSCsOriginHumanAdditional origin infoAge: 26-year-oldSex: MaleEthnicity: ThaiCell sourcePeripheral blood mononuclear cellsClonalityClonalMethod of reprogrammingIntegration-free Sendai viral vectorsGenetic modificationN/AType of modificationN/AAssociated diseaseN/AGene/locusN/AMethod of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock dateDecember 2014Cell line repository/bankN/AEthical approvalSiriraj Institutional Review Board (SiRB), no. Si248/2011, Faculty of Medicine Siriraj Hospital, Mahidol University, Thailand.http://www.sciencedirect.com/science/article/pii/S187350611830206X |
| spellingShingle | Ponthip Potirat Methichit Wattanapanitch Pakpoom Kheolamai Surapol Issaragrisil Establishment of a human iPSC line (MUSIi007-A) from peripheral blood of normal individual using Sendai viral vectors Stem Cell Research |
| title | Establishment of a human iPSC line (MUSIi007-A) from peripheral blood of normal individual using Sendai viral vectors |
| title_full | Establishment of a human iPSC line (MUSIi007-A) from peripheral blood of normal individual using Sendai viral vectors |
| title_fullStr | Establishment of a human iPSC line (MUSIi007-A) from peripheral blood of normal individual using Sendai viral vectors |
| title_full_unstemmed | Establishment of a human iPSC line (MUSIi007-A) from peripheral blood of normal individual using Sendai viral vectors |
| title_short | Establishment of a human iPSC line (MUSIi007-A) from peripheral blood of normal individual using Sendai viral vectors |
| title_sort | establishment of a human ipsc line musii007 a from peripheral blood of normal individual using sendai viral vectors |
| url | http://www.sciencedirect.com/science/article/pii/S187350611830206X |
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