Structural elucidation of phosphoglycolipids from strains of the bacterial thermophiles Thermus and Meiothermus
The structures of two major phosphoglycolipids from the thermophilic bacteria Thermus oshimai NTU-063, Thermus thermophilus NTU-077, Meiothermus ruber NTU-124, and Meiothermus taiwanensis NTU-220 were determined using spectroscopic and chemical analyses to be 2′-O-(1,2-diacyl-sn-glycero-3-phospho) -...
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Elsevier
2006-08-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520331679 |
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| author | Yu-Liang Yang Feng-Ling Yang Shu-Chuan Jao Mao-Yen Chen San-San Tsay Wei Zou Shih-Hsiung Wu |
| author_facet | Yu-Liang Yang Feng-Ling Yang Shu-Chuan Jao Mao-Yen Chen San-San Tsay Wei Zou Shih-Hsiung Wu |
| author_sort | Yu-Liang Yang |
| collection | DOAJ |
| description | The structures of two major phosphoglycolipids from the thermophilic bacteria Thermus oshimai NTU-063, Thermus thermophilus NTU-077, Meiothermus ruber NTU-124, and Meiothermus taiwanensis NTU-220 were determined using spectroscopic and chemical analyses to be 2′-O-(1,2-diacyl-sn-glycero-3-phospho) -3′-O-(α-N-acetyl-glucosaminyl)-N-glyceroyl alkylamine [PGL1 (1)] and the novel structure 2′-O-(2-acylalkyldio-1-O-phospho)-3′-O-(α-N-acetylglucosaminyl)-N-glyceroyl alkylamine [PGL2 (2)]. PGL2 (2) is the first phosphoglycolipid identified with a 2-acylalkyldio-1-O-phosphate moiety. The fatty acids of the phosphoglycolipids are mainly iso-C15:0, -C16:0, and -C17:0 and anteiso-C15:0 and -C17:0. The ratios of PGL2 (2) to PGL1 (1) are significantly altered when grown at different temperatures for three strains, T. thermophilus NTU-077, M. ruber NTU-124, and M. taiwanensis NTU-220, but not for T. oshimai NTU-063. Accordingly, the ratios of iso- to anteiso-branched fatty acids increase when grown at the higher temperature. |
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| id | doaj.art-7e244e333d3e41d18b5680c1b5e79372 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-20T05:40:33Z |
| publishDate | 2006-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-7e244e333d3e41d18b5680c1b5e793722022-12-21T19:51:28ZengElsevierJournal of Lipid Research0022-22752006-08-0147818231832Structural elucidation of phosphoglycolipids from strains of the bacterial thermophiles Thermus and MeiothermusYu-Liang Yang0Feng-Ling Yang1Shu-Chuan Jao2Mao-Yen Chen3San-San Tsay4Wei Zou5Shih-Hsiung Wu6Institute of Biological Chemistry, Academia Sinica, Taipei 115, TaiwanInstitute of Biological Chemistry, Academia Sinica, Taipei 115, TaiwanInstitute of Biological Chemistry, Academia Sinica, Taipei 115, TaiwanInstitute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan; Department of Life Science and Institute of Plant Biology, National Taiwan University, Taipei 106, TaiwanDepartment of Life Science and Institute of Plant Biology, National Taiwan University, Taipei 106, TaiwanInstitute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, CanadaInstitute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan; Institute of Biochemical Sciences, National Taiwan University, Taipei 106, TaiwanThe structures of two major phosphoglycolipids from the thermophilic bacteria Thermus oshimai NTU-063, Thermus thermophilus NTU-077, Meiothermus ruber NTU-124, and Meiothermus taiwanensis NTU-220 were determined using spectroscopic and chemical analyses to be 2′-O-(1,2-diacyl-sn-glycero-3-phospho) -3′-O-(α-N-acetyl-glucosaminyl)-N-glyceroyl alkylamine [PGL1 (1)] and the novel structure 2′-O-(2-acylalkyldio-1-O-phospho)-3′-O-(α-N-acetylglucosaminyl)-N-glyceroyl alkylamine [PGL2 (2)]. PGL2 (2) is the first phosphoglycolipid identified with a 2-acylalkyldio-1-O-phosphate moiety. The fatty acids of the phosphoglycolipids are mainly iso-C15:0, -C16:0, and -C17:0 and anteiso-C15:0 and -C17:0. The ratios of PGL2 (2) to PGL1 (1) are significantly altered when grown at different temperatures for three strains, T. thermophilus NTU-077, M. ruber NTU-124, and M. taiwanensis NTU-220, but not for T. oshimai NTU-063. Accordingly, the ratios of iso- to anteiso-branched fatty acids increase when grown at the higher temperature.http://www.sciencedirect.com/science/article/pii/S0022227520331679nuclear magnetic resonance spectroscopymatrix-assisted laser desorption ionization mass spectroscopycapillary electrophoresis-mass spectroscopytandem mass spectrometryThermus oshimai NTU-063Thermus thermophilus NTU-077 |
| spellingShingle | Yu-Liang Yang Feng-Ling Yang Shu-Chuan Jao Mao-Yen Chen San-San Tsay Wei Zou Shih-Hsiung Wu Structural elucidation of phosphoglycolipids from strains of the bacterial thermophiles Thermus and Meiothermus Journal of Lipid Research nuclear magnetic resonance spectroscopy matrix-assisted laser desorption ionization mass spectroscopy capillary electrophoresis-mass spectroscopy tandem mass spectrometry Thermus oshimai NTU-063 Thermus thermophilus NTU-077 |
| title | Structural elucidation of phosphoglycolipids from strains of the bacterial thermophiles Thermus and Meiothermus |
| title_full | Structural elucidation of phosphoglycolipids from strains of the bacterial thermophiles Thermus and Meiothermus |
| title_fullStr | Structural elucidation of phosphoglycolipids from strains of the bacterial thermophiles Thermus and Meiothermus |
| title_full_unstemmed | Structural elucidation of phosphoglycolipids from strains of the bacterial thermophiles Thermus and Meiothermus |
| title_short | Structural elucidation of phosphoglycolipids from strains of the bacterial thermophiles Thermus and Meiothermus |
| title_sort | structural elucidation of phosphoglycolipids from strains of the bacterial thermophiles thermus and meiothermus |
| topic | nuclear magnetic resonance spectroscopy matrix-assisted laser desorption ionization mass spectroscopy capillary electrophoresis-mass spectroscopy tandem mass spectrometry Thermus oshimai NTU-063 Thermus thermophilus NTU-077 |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520331679 |
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