Sperm cryopreservation of lane snapper Lutjanus synagris(Linnaeus, 1758)
AbstractThis study aims developing and evaluate a protocol of semen cryopreservation of the lane snapper Lutjanus synagris. Firstly, sperm motility rate, motility time, density and spermatocrit were appraised to characterize the sperm quality of the lane snapper. The effect of three extenders with d...
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| Format: | Article |
| Language: | English |
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Instituto Internacional de Ecologia
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| Series: | Brazilian Journal of Biology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842015000400662&lng=en&tlng=en |
| _version_ | 1818239862257483776 |
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| author | EG Sanches IR Oliveira PCS Serralheiro VR Cerqueira |
| author_facet | EG Sanches IR Oliveira PCS Serralheiro VR Cerqueira |
| author_sort | EG Sanches |
| collection | DOAJ |
| description | AbstractThis study aims developing and evaluate a protocol of semen cryopreservation of the lane snapper Lutjanus synagris. Firstly, sperm motility rate, motility time, density and spermatocrit were appraised to characterize the sperm quality of the lane snapper. The effect of three extenders with distinct ionic compositions and pH values combined with seven concentrations of cryoprotector dimethylsulfoxide (0; 2.5; 5.0; 7.5; 10.0; 12.5 e 15.0%), five cooling rates (110, 90, 60, 45 e 30°C –min), nine equilibration time (1; 2,5; 5; 10; 15; 20; 25; 30 e 60 minutes) e five dilutions ratio (1:1; 1:3; 1:6; 1:10 e 1:20) on the sperm motility rate and motility time were analyzed. Fertilization test was accomplished to evaluate the viability of the cryopreserved sperm. The higher sperm motility rate and motility time (P<0.05) was achieved by combining extender with pH 8.2 with 10% concentration of dimethylsulfoxide and cooling rate 60°C –min, 1 minute of equilibration time and 1:3 (v/v) dilution ratio. The use of cryopreserved sperm presented fertilization rates >60% validating the present protocol for lane snapper. The cryoconserved sperm of lane snapper is a viable alternative, being possible to maintain appropriate sperm viability. |
| first_indexed | 2024-12-12T13:04:17Z |
| format | Article |
| id | doaj.art-7e250fc5e8f3417c8ac4bb5b90d92f1c |
| institution | Directory Open Access Journal |
| issn | 1678-4375 |
| language | English |
| last_indexed | 2024-12-12T13:04:17Z |
| publisher | Instituto Internacional de Ecologia |
| record_format | Article |
| series | Brazilian Journal of Biology |
| spelling | doaj.art-7e250fc5e8f3417c8ac4bb5b90d92f1c2022-12-22T00:23:41ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-437575366266910.1590/1519-6984.20613S1519-69842015000400662Sperm cryopreservation of lane snapper Lutjanus synagris(Linnaeus, 1758)EG SanchesIR OliveiraPCS SerralheiroVR CerqueiraAbstractThis study aims developing and evaluate a protocol of semen cryopreservation of the lane snapper Lutjanus synagris. Firstly, sperm motility rate, motility time, density and spermatocrit were appraised to characterize the sperm quality of the lane snapper. The effect of three extenders with distinct ionic compositions and pH values combined with seven concentrations of cryoprotector dimethylsulfoxide (0; 2.5; 5.0; 7.5; 10.0; 12.5 e 15.0%), five cooling rates (110, 90, 60, 45 e 30°C –min), nine equilibration time (1; 2,5; 5; 10; 15; 20; 25; 30 e 60 minutes) e five dilutions ratio (1:1; 1:3; 1:6; 1:10 e 1:20) on the sperm motility rate and motility time were analyzed. Fertilization test was accomplished to evaluate the viability of the cryopreserved sperm. The higher sperm motility rate and motility time (P<0.05) was achieved by combining extender with pH 8.2 with 10% concentration of dimethylsulfoxide and cooling rate 60°C –min, 1 minute of equilibration time and 1:3 (v/v) dilution ratio. The use of cryopreserved sperm presented fertilization rates >60% validating the present protocol for lane snapper. The cryoconserved sperm of lane snapper is a viable alternative, being possible to maintain appropriate sperm viability.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842015000400662&lng=en&tlng=enextendercryoprotectorLutjanus synagrisreproductionmariculture |
| spellingShingle | EG Sanches IR Oliveira PCS Serralheiro VR Cerqueira Sperm cryopreservation of lane snapper Lutjanus synagris(Linnaeus, 1758) Brazilian Journal of Biology extender cryoprotector Lutjanus synagris reproduction mariculture |
| title | Sperm cryopreservation of lane snapper Lutjanus synagris(Linnaeus, 1758) |
| title_full | Sperm cryopreservation of lane snapper Lutjanus synagris(Linnaeus, 1758) |
| title_fullStr | Sperm cryopreservation of lane snapper Lutjanus synagris(Linnaeus, 1758) |
| title_full_unstemmed | Sperm cryopreservation of lane snapper Lutjanus synagris(Linnaeus, 1758) |
| title_short | Sperm cryopreservation of lane snapper Lutjanus synagris(Linnaeus, 1758) |
| title_sort | sperm cryopreservation of lane snapper lutjanus synagris linnaeus 1758 |
| topic | extender cryoprotector Lutjanus synagris reproduction mariculture |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842015000400662&lng=en&tlng=en |
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