Effect of Different Parameters of In Vitro Static Tensile Strain on Human Periodontal Ligament Cells Simulating the Tension Side of Orthodontic Tooth Movement
This study aimed to investigate the effects of different magnitudes and durations of static tensile strain on human periodontal ligament cells (hPDLCs), focusing on osteogenesis, mechanosensing and inflammation. Static tensile strain magnitudes of 0%, 3%, 6%, 10%, 15% and 20% were applied to hPDLCs...
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| Format: | Article |
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MDPI AG
2022-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/3/1525 |
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| author | Changyun Sun Mila Janjic Rankovic Matthias Folwaczny Thomas Stocker Sven Otto Andrea Wichelhaus Uwe Baumert |
| author_facet | Changyun Sun Mila Janjic Rankovic Matthias Folwaczny Thomas Stocker Sven Otto Andrea Wichelhaus Uwe Baumert |
| author_sort | Changyun Sun |
| collection | DOAJ |
| description | This study aimed to investigate the effects of different magnitudes and durations of static tensile strain on human periodontal ligament cells (hPDLCs), focusing on osteogenesis, mechanosensing and inflammation. Static tensile strain magnitudes of 0%, 3%, 6%, 10%, 15% and 20% were applied to hPDLCs for 1, 2 and 3 days. Cell viability was confirmed via live/dead cell staining. Reference genes were tested by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) and assessed. The expressions of <i>TNFRSF11B</i>, <i>ALPL</i>, <i>RUNX2</i>, <i>BGLAP</i>, <i>SP7</i>, <i>FOS</i>, <i>IL6</i>, <i>PTGS2</i>, <i>TNF</i>, IL1B, IL8, IL10 and PGE2 were analyzed by RT-qPCR and/or enzyme-linked immunosorbent assay (ELISA). <i>ALPL</i> and <i>RUNX2</i> both peaked after 1 day, reaching their maximum at 3%, whereas <i>BGLAP</i> peaked after 3 days with its maximum at 10%. <i>SP7</i> peaked after 1 day at 6%, 10% and 15%. <i>FOS</i> peaked after 3 days with its maximum at 3%, 6% and 15%. The expressions of <i>IL6</i> and <i>PTGS2</i> both peaked after 1 day, with their minimum at 10%. PGE2 peaked after 1 day (maximum at 20%). The ELISA of IL6 peaked after 3 days, with the minimum at 10%. In summary, the lower magnitudes promoted osteogenesis and caused less inflammation, while the higher magnitudes inhibited osteogenesis and enhanced inflammation. Among all magnitudes, 10% generally caused a lower level of inflammation with a higher level of osteogenesis. |
| first_indexed | 2024-03-09T23:46:49Z |
| format | Article |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:46:49Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7e26ed8c56624126a2da4a17554640ea2023-11-23T16:41:54ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-01-01233152510.3390/ijms23031525Effect of Different Parameters of In Vitro Static Tensile Strain on Human Periodontal Ligament Cells Simulating the Tension Side of Orthodontic Tooth MovementChangyun Sun0Mila Janjic Rankovic1Matthias Folwaczny2Thomas Stocker3Sven Otto4Andrea Wichelhaus5Uwe Baumert6Department of Orthodontics and Dentofacial Orthopedics, University Hospital, LMU Munich, 80336 Munich, GermanyDepartment of Orthodontics and Dentofacial Orthopedics, University Hospital, LMU Munich, 80336 Munich, GermanyDepartment of Conservative Dentistry and Periodontology, University Hospital, LMU Munich, 80336 Munich, GermanyDepartment of Orthodontics and Dentofacial Orthopedics, University Hospital, LMU Munich, 80336 Munich, GermanyDepartment of Oral and Maxillofacial Surgery and Facial Plastic Surgery, University Hospital, LMU Munich, 80336 Munich, GermanyDepartment of Orthodontics and Dentofacial Orthopedics, University Hospital, LMU Munich, 80336 Munich, GermanyDepartment of Orthodontics and Dentofacial Orthopedics, University Hospital, LMU Munich, 80336 Munich, GermanyThis study aimed to investigate the effects of different magnitudes and durations of static tensile strain on human periodontal ligament cells (hPDLCs), focusing on osteogenesis, mechanosensing and inflammation. Static tensile strain magnitudes of 0%, 3%, 6%, 10%, 15% and 20% were applied to hPDLCs for 1, 2 and 3 days. Cell viability was confirmed via live/dead cell staining. Reference genes were tested by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) and assessed. The expressions of <i>TNFRSF11B</i>, <i>ALPL</i>, <i>RUNX2</i>, <i>BGLAP</i>, <i>SP7</i>, <i>FOS</i>, <i>IL6</i>, <i>PTGS2</i>, <i>TNF</i>, IL1B, IL8, IL10 and PGE2 were analyzed by RT-qPCR and/or enzyme-linked immunosorbent assay (ELISA). <i>ALPL</i> and <i>RUNX2</i> both peaked after 1 day, reaching their maximum at 3%, whereas <i>BGLAP</i> peaked after 3 days with its maximum at 10%. <i>SP7</i> peaked after 1 day at 6%, 10% and 15%. <i>FOS</i> peaked after 3 days with its maximum at 3%, 6% and 15%. The expressions of <i>IL6</i> and <i>PTGS2</i> both peaked after 1 day, with their minimum at 10%. PGE2 peaked after 1 day (maximum at 20%). The ELISA of IL6 peaked after 3 days, with the minimum at 10%. In summary, the lower magnitudes promoted osteogenesis and caused less inflammation, while the higher magnitudes inhibited osteogenesis and enhanced inflammation. Among all magnitudes, 10% generally caused a lower level of inflammation with a higher level of osteogenesis.https://www.mdpi.com/1422-0067/23/3/1525periodontal ligament cellstensile strainbone remodelingstretchingorthodontic tooth movement |
| spellingShingle | Changyun Sun Mila Janjic Rankovic Matthias Folwaczny Thomas Stocker Sven Otto Andrea Wichelhaus Uwe Baumert Effect of Different Parameters of In Vitro Static Tensile Strain on Human Periodontal Ligament Cells Simulating the Tension Side of Orthodontic Tooth Movement International Journal of Molecular Sciences periodontal ligament cells tensile strain bone remodeling stretching orthodontic tooth movement |
| title | Effect of Different Parameters of In Vitro Static Tensile Strain on Human Periodontal Ligament Cells Simulating the Tension Side of Orthodontic Tooth Movement |
| title_full | Effect of Different Parameters of In Vitro Static Tensile Strain on Human Periodontal Ligament Cells Simulating the Tension Side of Orthodontic Tooth Movement |
| title_fullStr | Effect of Different Parameters of In Vitro Static Tensile Strain on Human Periodontal Ligament Cells Simulating the Tension Side of Orthodontic Tooth Movement |
| title_full_unstemmed | Effect of Different Parameters of In Vitro Static Tensile Strain on Human Periodontal Ligament Cells Simulating the Tension Side of Orthodontic Tooth Movement |
| title_short | Effect of Different Parameters of In Vitro Static Tensile Strain on Human Periodontal Ligament Cells Simulating the Tension Side of Orthodontic Tooth Movement |
| title_sort | effect of different parameters of in vitro static tensile strain on human periodontal ligament cells simulating the tension side of orthodontic tooth movement |
| topic | periodontal ligament cells tensile strain bone remodeling stretching orthodontic tooth movement |
| url | https://www.mdpi.com/1422-0067/23/3/1525 |
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