Novel acaricidal and growth-regulating activity of Aloe vera and Rheum rhabarbarum extracts and their oil/water nanoemulsions against the camel tick, Hyalomma dromedarii
Abstract Hyalomma dromedarii is an important tick species infesting livestock. This work evaluated the novel adulticidal, insect growth-regulating, and enzymatic efficacy of ethanol plant extracts of Aloe vera and Rheum rhabarbarum and their nanoemulsions against males and engorged females of the ca...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-43776-6 |
| _version_ | 1827634400603406336 |
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| author | Ibrahim T. Radwan Randa I. Eltaly Mohamed M. Baz Mohamed Yousif Abdelfattah Selim Hanan A. A. Taie Eman A. Manaa Hanem F. Khater |
| author_facet | Ibrahim T. Radwan Randa I. Eltaly Mohamed M. Baz Mohamed Yousif Abdelfattah Selim Hanan A. A. Taie Eman A. Manaa Hanem F. Khater |
| author_sort | Ibrahim T. Radwan |
| collection | DOAJ |
| description | Abstract Hyalomma dromedarii is an important tick species infesting livestock. This work evaluated the novel adulticidal, insect growth-regulating, and enzymatic efficacy of ethanol plant extracts of Aloe vera and Rheum rhabarbarum and their nanoemulsions against males and engorged females of the camel tick, H. dromedarii. The physicochemical properties of nanoemulsions were evaluated. The High-Performance Liquid Chromatography (HPLC) analyses indicated that the extracts contained polyphenols and flavonoids, which could enhance their acaricidal effect. Dynamic light scattering (DLS) of the nanoemulsions of A. vera and R. rhabarbarum were 196.7 and 291 nm, whereas their zeta potentials were − 29.1 and − 53.1 mV, respectively. Transmission electron microscope (TEM) indicated that nanoemulsions showed a regular spherical shape (less than 100 nm). Fifteen days post-treatment (PT) with 25%, the mortality% of A. vera and R. rhabarbarum were 88.5 and 96.2%, respectively. Five days PT, the median lethal concentration values of A. vera, R. rhabarbarum, and their nanoemulsions were 7.8, 7.1, 2.8, and 1.02%, respectively, and their toxicity indices were 91.02, 100, 36.4, and 100%, respectively. Their median lethal time values PT with 3.5% were 6.09, 5.09, 1.75, and 1.34 days, respectively. Nanoemulsions enhanced the efficacy of the crude extract 1–7 folds, 5 days PT, and accelerated their speed of killing ticks 2–4 times. The total protein and carbohydrates, Acetylcholinesterase, Alpha esterase, and Amylase were affected PT. The reproductive potential of engorged females was adversely impacted. In conclusion, the novel A. vera and R. rhabarbarum extracts were promising acaricides, and their nanoformulations enhanced their efficacies. |
| first_indexed | 2024-03-09T15:13:44Z |
| format | Article |
| id | doaj.art-7e278264f5284bc096daa13e7559378c |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:13:44Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-7e278264f5284bc096daa13e7559378c2023-11-26T13:14:40ZengNature PortfolioScientific Reports2045-23222023-10-0113111810.1038/s41598-023-43776-6Novel acaricidal and growth-regulating activity of Aloe vera and Rheum rhabarbarum extracts and their oil/water nanoemulsions against the camel tick, Hyalomma dromedariiIbrahim T. Radwan0Randa I. Eltaly1Mohamed M. Baz2Mohamed Yousif3Abdelfattah Selim4Hanan A. A. Taie5Eman A. Manaa6Hanem F. Khater7Supplementary General Sciences Department, Faculty of Oral and Dental Medicine, Future University in EgyptZoology and Entomology Department, Faculty of Science, Al-Azhar UniversityDepartment of Entomology, Faculty of Science, Benha UniversityParasitology Department, Faculty of Veterinary Medicine, Benha UniversityDepartment of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Benha UniversityPlant Biochemistry Department, Agricultural and Biology Research Institute, National Research CenterAnimal and Poultry Production, Department of Animal Wealth Development, Faculty of Veterinary Medicine, Benha UniversityParasitology Department, Faculty of Veterinary Medicine, Benha UniversityAbstract Hyalomma dromedarii is an important tick species infesting livestock. This work evaluated the novel adulticidal, insect growth-regulating, and enzymatic efficacy of ethanol plant extracts of Aloe vera and Rheum rhabarbarum and their nanoemulsions against males and engorged females of the camel tick, H. dromedarii. The physicochemical properties of nanoemulsions were evaluated. The High-Performance Liquid Chromatography (HPLC) analyses indicated that the extracts contained polyphenols and flavonoids, which could enhance their acaricidal effect. Dynamic light scattering (DLS) of the nanoemulsions of A. vera and R. rhabarbarum were 196.7 and 291 nm, whereas their zeta potentials were − 29.1 and − 53.1 mV, respectively. Transmission electron microscope (TEM) indicated that nanoemulsions showed a regular spherical shape (less than 100 nm). Fifteen days post-treatment (PT) with 25%, the mortality% of A. vera and R. rhabarbarum were 88.5 and 96.2%, respectively. Five days PT, the median lethal concentration values of A. vera, R. rhabarbarum, and their nanoemulsions were 7.8, 7.1, 2.8, and 1.02%, respectively, and their toxicity indices were 91.02, 100, 36.4, and 100%, respectively. Their median lethal time values PT with 3.5% were 6.09, 5.09, 1.75, and 1.34 days, respectively. Nanoemulsions enhanced the efficacy of the crude extract 1–7 folds, 5 days PT, and accelerated their speed of killing ticks 2–4 times. The total protein and carbohydrates, Acetylcholinesterase, Alpha esterase, and Amylase were affected PT. The reproductive potential of engorged females was adversely impacted. In conclusion, the novel A. vera and R. rhabarbarum extracts were promising acaricides, and their nanoformulations enhanced their efficacies.https://doi.org/10.1038/s41598-023-43776-6 |
| spellingShingle | Ibrahim T. Radwan Randa I. Eltaly Mohamed M. Baz Mohamed Yousif Abdelfattah Selim Hanan A. A. Taie Eman A. Manaa Hanem F. Khater Novel acaricidal and growth-regulating activity of Aloe vera and Rheum rhabarbarum extracts and their oil/water nanoemulsions against the camel tick, Hyalomma dromedarii Scientific Reports |
| title | Novel acaricidal and growth-regulating activity of Aloe vera and Rheum rhabarbarum extracts and their oil/water nanoemulsions against the camel tick, Hyalomma dromedarii |
| title_full | Novel acaricidal and growth-regulating activity of Aloe vera and Rheum rhabarbarum extracts and their oil/water nanoemulsions against the camel tick, Hyalomma dromedarii |
| title_fullStr | Novel acaricidal and growth-regulating activity of Aloe vera and Rheum rhabarbarum extracts and their oil/water nanoemulsions against the camel tick, Hyalomma dromedarii |
| title_full_unstemmed | Novel acaricidal and growth-regulating activity of Aloe vera and Rheum rhabarbarum extracts and their oil/water nanoemulsions against the camel tick, Hyalomma dromedarii |
| title_short | Novel acaricidal and growth-regulating activity of Aloe vera and Rheum rhabarbarum extracts and their oil/water nanoemulsions against the camel tick, Hyalomma dromedarii |
| title_sort | novel acaricidal and growth regulating activity of aloe vera and rheum rhabarbarum extracts and their oil water nanoemulsions against the camel tick hyalomma dromedarii |
| url | https://doi.org/10.1038/s41598-023-43776-6 |
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