14-3-3<i>η</i> Protein as a Potential Biomarker in Juvenile Idiopathic Arthritis
The 14-3-3<b><i>η</i></b> (eta) protein was evaluated as a biomarker in a cohort of patients with juvenile idiopathic arthritis (JIA), as well as disease- and healthy-controls, to determine its potential clinical utility. In this case-control study, levels of 14-3-3<b>&...
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MDPI AG
2021-01-01
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| Series: | Pediatric Reports |
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| Online Access: | https://www.mdpi.com/2036-7503/13/1/8 |
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| author | Austin Dalrymple Paul Tuttle Lance Feller Olga Zhukov Robert Lagier Joanna Popov Stanley Naides Terry Moore |
| author_facet | Austin Dalrymple Paul Tuttle Lance Feller Olga Zhukov Robert Lagier Joanna Popov Stanley Naides Terry Moore |
| author_sort | Austin Dalrymple |
| collection | DOAJ |
| description | The 14-3-3<b><i>η</i></b> (eta) protein was evaluated as a biomarker in a cohort of patients with juvenile idiopathic arthritis (JIA), as well as disease- and healthy-controls, to determine its potential clinical utility. In this case-control study, levels of 14-3-3<b><i>η</i></b> protein were evaluated in archival specimens from patients with JIA, systemic lupus erythematosus (SLE), and rheumatoid arthritis (RA), as well as healthy pediatric controls. Just over 200 patients were evaluated, using specimens banked between 1990 and 2011. Comparisons were made to complete blood cell count (CBC), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), rheumatoid factor (RF), anti-cyclic citrullinated peptide (anti-CCP) antibodies, and anti-nuclear antibody (ANA) positivity. 14-3-3<b><i>η</i></b> at levels 0.2 ng/mL or higher was considered positive. Fisher’s exact tests, odds ratios, 95% confidence intervals, and p-values were reported. 14-3-3<b><i>η</i></b> positivity was seen in all included JIA subtypes. The rate of positivity was the highest in RF-positive (pos) polyarticular JIA. In the disease and healthy controls, lower rates of positivity were observed. The frequency of 14-3-3<b><i>η</i></b> positivity among RF-positive and RF-negative (neg) polyarticular JIA patients, especially at values ≥0.5 ng/mL (associated with poor outcomes in adults), was also highest. Several JIA patients with 14-3-3<b><i>η</i></b> positivity developed RF and anti-CCP positivity later in their disease. Significant levels of 14-3-3<b><i>η</i></b> can be found in approximately 30% of RF-pos and RF-neg patients with polyarticular JIA. This protein may represent a new biomarker for polyarticular JIA, particularly RF-neg polyarticular JIA. |
| first_indexed | 2024-03-09T03:43:31Z |
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| institution | Directory Open Access Journal |
| issn | 2036-7503 |
| language | English |
| last_indexed | 2024-03-09T03:43:31Z |
| publishDate | 2021-01-01 |
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| series | Pediatric Reports |
| spelling | doaj.art-7e28316fff9e4303b835c18b26c8b4fb2023-12-03T14:37:28ZengMDPI AGPediatric Reports2036-75032021-01-01131657110.3390/pediatric1301000814-3-3<i>η</i> Protein as a Potential Biomarker in Juvenile Idiopathic ArthritisAustin Dalrymple0Paul Tuttle1Lance Feller2Olga Zhukov3Robert Lagier4Joanna Popov5Stanley Naides6Terry Moore7Division of Adult & Pediatric Rheumatology, Saint Louis University School of Medicine & SSM Health Cardinal Glennon Children’s Hospital, Saint Louis, MO 63104, USADivision of Adult & Pediatric Rheumatology, Saint Louis University School of Medicine & SSM Health Cardinal Glennon Children’s Hospital, Saint Louis, MO 63104, USADivision of Adult & Pediatric Rheumatology, Saint Louis University School of Medicine & SSM Health Cardinal Glennon Children’s Hospital, Saint Louis, MO 63104, USAQuest Diagnostics Nichols Institute, San Juan Capistrano, CA 92675, USAQuest Diagnostics Nichols Institute, San Juan Capistrano, CA 92675, USAQuest Diagnostics Nichols Institute, San Juan Capistrano, CA 92675, USAQuest Diagnostics Nichols Institute, San Juan Capistrano, CA 92675, USADivision of Adult & Pediatric Rheumatology, Saint Louis University School of Medicine & SSM Health Cardinal Glennon Children’s Hospital, Saint Louis, MO 63104, USAThe 14-3-3<b><i>η</i></b> (eta) protein was evaluated as a biomarker in a cohort of patients with juvenile idiopathic arthritis (JIA), as well as disease- and healthy-controls, to determine its potential clinical utility. In this case-control study, levels of 14-3-3<b><i>η</i></b> protein were evaluated in archival specimens from patients with JIA, systemic lupus erythematosus (SLE), and rheumatoid arthritis (RA), as well as healthy pediatric controls. Just over 200 patients were evaluated, using specimens banked between 1990 and 2011. Comparisons were made to complete blood cell count (CBC), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), rheumatoid factor (RF), anti-cyclic citrullinated peptide (anti-CCP) antibodies, and anti-nuclear antibody (ANA) positivity. 14-3-3<b><i>η</i></b> at levels 0.2 ng/mL or higher was considered positive. Fisher’s exact tests, odds ratios, 95% confidence intervals, and p-values were reported. 14-3-3<b><i>η</i></b> positivity was seen in all included JIA subtypes. The rate of positivity was the highest in RF-positive (pos) polyarticular JIA. In the disease and healthy controls, lower rates of positivity were observed. The frequency of 14-3-3<b><i>η</i></b> positivity among RF-positive and RF-negative (neg) polyarticular JIA patients, especially at values ≥0.5 ng/mL (associated with poor outcomes in adults), was also highest. Several JIA patients with 14-3-3<b><i>η</i></b> positivity developed RF and anti-CCP positivity later in their disease. Significant levels of 14-3-3<b><i>η</i></b> can be found in approximately 30% of RF-pos and RF-neg patients with polyarticular JIA. This protein may represent a new biomarker for polyarticular JIA, particularly RF-neg polyarticular JIA.https://www.mdpi.com/2036-7503/13/1/8juvenile idiopathic arthritis14-3-3 proteinsbiomarkers |
| spellingShingle | Austin Dalrymple Paul Tuttle Lance Feller Olga Zhukov Robert Lagier Joanna Popov Stanley Naides Terry Moore 14-3-3<i>η</i> Protein as a Potential Biomarker in Juvenile Idiopathic Arthritis Pediatric Reports juvenile idiopathic arthritis 14-3-3 proteins biomarkers |
| title | 14-3-3<i>η</i> Protein as a Potential Biomarker in Juvenile Idiopathic Arthritis |
| title_full | 14-3-3<i>η</i> Protein as a Potential Biomarker in Juvenile Idiopathic Arthritis |
| title_fullStr | 14-3-3<i>η</i> Protein as a Potential Biomarker in Juvenile Idiopathic Arthritis |
| title_full_unstemmed | 14-3-3<i>η</i> Protein as a Potential Biomarker in Juvenile Idiopathic Arthritis |
| title_short | 14-3-3<i>η</i> Protein as a Potential Biomarker in Juvenile Idiopathic Arthritis |
| title_sort | 14 3 3 i η i protein as a potential biomarker in juvenile idiopathic arthritis |
| topic | juvenile idiopathic arthritis 14-3-3 proteins biomarkers |
| url | https://www.mdpi.com/2036-7503/13/1/8 |
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