Severe oxidative stress in sickle cell disease patients with uncomplicated Plasmodium falciparum malaria in Kampala, Uganda
Abstract Background Oxidative stress plays a vital role in the pathogenesis of both Sickle Cell Disease (SCD) and Plasmodium falciparum malaria. However, there are limited studies on the effect of P. falciparum malaria infection on oxidative stress in SCD patients. Methods A cross-sectional study wa...
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| Format: | Article |
| Language: | English |
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BMC
2019-07-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | http://link.springer.com/article/10.1186/s12879-019-4221-y |
| _version_ | 1818413107107594240 |
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| author | Saad Mahjub Atiku Nabukeera Louise Dennis M. Kasozi |
| author_facet | Saad Mahjub Atiku Nabukeera Louise Dennis M. Kasozi |
| author_sort | Saad Mahjub Atiku |
| collection | DOAJ |
| description | Abstract Background Oxidative stress plays a vital role in the pathogenesis of both Sickle Cell Disease (SCD) and Plasmodium falciparum malaria. However, there are limited studies on the effect of P. falciparum malaria infection on oxidative stress in SCD patients. Methods A cross-sectional study was undertaken to compare levels of biomarkers of oxidative stress in isolates from SCD patients with uncomplicated P.falciparum malaria. The biomarkers namely: malondialdehyde (MDA), reduced glutathione (GSH), catalase (CAT) and glutathione peroxidase (GPx) were determined in plasma samples from SCD malaria positive, malaria positive, SCD malaria negative and healthy control participants. The genetic diversity of P.falciparum was determined by nested polymerase chain reaction of merozoite surface protein-2 (MSP-2) gene. Results Out of 207 participants, 54 (26%) were SCD malaria positive, 51 (24%) malaria positive, 51 (24%) SCD controls and 51 (24%) healthy control individuals. The mean concentration of MDA was significantly higher in SCD malaria positive than SCD controls (P < 0.0001). In contrast, the mean concentration of GSH (P < 0.0001) and GPx (P < 0.0001) were significantly lower in SCD malaria than SCD controls. Although not significantly different, the mean concentration of MDA was higher (P = 0.0478), but the geometric mean parasite density (P = 0.2430) and multiplicity of infection (P = 0.3478) were lower in SCD malaria samples than in malaria samples. The most prevalent MSP2 allelic family was IC3D7 in SCD malaria (72%) and Malaria (76%) samples. The biomarkers of oxidative stress were not significantly different between IC3D7 and FC27 allelic families of MSP2. Conclusion We identified severe oxidative stress in Sickle cell disease patients with uncomplicated P.falciparum malaria. |
| first_indexed | 2024-12-14T10:57:56Z |
| format | Article |
| id | doaj.art-7e29c22f00e74f41856fa3d88d88609a |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-14T10:57:56Z |
| publishDate | 2019-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-7e29c22f00e74f41856fa3d88d88609a2022-12-21T23:04:51ZengBMCBMC Infectious Diseases1471-23342019-07-0119111010.1186/s12879-019-4221-ySevere oxidative stress in sickle cell disease patients with uncomplicated Plasmodium falciparum malaria in Kampala, UgandaSaad Mahjub Atiku0Nabukeera Louise1Dennis M. Kasozi2Department of Biochemistry and Sports Science, School of Biosciences, College of Natural Sciences, Makerere University KampalaDepartment of Biochemistry and Sports Science, School of Biosciences, College of Natural Sciences, Makerere University KampalaDepartment of Biochemistry and Sports Science, School of Biosciences, College of Natural Sciences, Makerere University KampalaAbstract Background Oxidative stress plays a vital role in the pathogenesis of both Sickle Cell Disease (SCD) and Plasmodium falciparum malaria. However, there are limited studies on the effect of P. falciparum malaria infection on oxidative stress in SCD patients. Methods A cross-sectional study was undertaken to compare levels of biomarkers of oxidative stress in isolates from SCD patients with uncomplicated P.falciparum malaria. The biomarkers namely: malondialdehyde (MDA), reduced glutathione (GSH), catalase (CAT) and glutathione peroxidase (GPx) were determined in plasma samples from SCD malaria positive, malaria positive, SCD malaria negative and healthy control participants. The genetic diversity of P.falciparum was determined by nested polymerase chain reaction of merozoite surface protein-2 (MSP-2) gene. Results Out of 207 participants, 54 (26%) were SCD malaria positive, 51 (24%) malaria positive, 51 (24%) SCD controls and 51 (24%) healthy control individuals. The mean concentration of MDA was significantly higher in SCD malaria positive than SCD controls (P < 0.0001). In contrast, the mean concentration of GSH (P < 0.0001) and GPx (P < 0.0001) were significantly lower in SCD malaria than SCD controls. Although not significantly different, the mean concentration of MDA was higher (P = 0.0478), but the geometric mean parasite density (P = 0.2430) and multiplicity of infection (P = 0.3478) were lower in SCD malaria samples than in malaria samples. The most prevalent MSP2 allelic family was IC3D7 in SCD malaria (72%) and Malaria (76%) samples. The biomarkers of oxidative stress were not significantly different between IC3D7 and FC27 allelic families of MSP2. Conclusion We identified severe oxidative stress in Sickle cell disease patients with uncomplicated P.falciparum malaria.http://link.springer.com/article/10.1186/s12879-019-4221-ySickle cell diseaseMalariaBiomarkers of oxidative stressMalondialdehydeCatalaseUganda |
| spellingShingle | Saad Mahjub Atiku Nabukeera Louise Dennis M. Kasozi Severe oxidative stress in sickle cell disease patients with uncomplicated Plasmodium falciparum malaria in Kampala, Uganda BMC Infectious Diseases Sickle cell disease Malaria Biomarkers of oxidative stress Malondialdehyde Catalase Uganda |
| title | Severe oxidative stress in sickle cell disease patients with uncomplicated Plasmodium falciparum malaria in Kampala, Uganda |
| title_full | Severe oxidative stress in sickle cell disease patients with uncomplicated Plasmodium falciparum malaria in Kampala, Uganda |
| title_fullStr | Severe oxidative stress in sickle cell disease patients with uncomplicated Plasmodium falciparum malaria in Kampala, Uganda |
| title_full_unstemmed | Severe oxidative stress in sickle cell disease patients with uncomplicated Plasmodium falciparum malaria in Kampala, Uganda |
| title_short | Severe oxidative stress in sickle cell disease patients with uncomplicated Plasmodium falciparum malaria in Kampala, Uganda |
| title_sort | severe oxidative stress in sickle cell disease patients with uncomplicated plasmodium falciparum malaria in kampala uganda |
| topic | Sickle cell disease Malaria Biomarkers of oxidative stress Malondialdehyde Catalase Uganda |
| url | http://link.springer.com/article/10.1186/s12879-019-4221-y |
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