Utilisation of in vitro Techniques in Rescue of Gene Resources of Meadow Vetchling (Lathyrus pratensis L.)
In the case of poor germination of seed samples and minimal number of seedlings obtained, in vitro methods can be used to revitalise and recover the gene resource. The highest germination of meadow vetchling (Lathyrus pratensis L.) seeds was achieved after scarification with H2SO4 and cultivation in...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2003-09-01
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| Series: | Czech Journal of Genetics and Plant Breeding |
| Subjects: | |
| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-200303-0003_utilisation-of-in-vitro-techniques-in-rescue-of-gene-resources-of-meadow-vetchling-lathyrus-pratensis-l.php |
| _version_ | 1797899546550861824 |
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| author | L. Klčová M. Gubišová |
| author_facet | L. Klčová M. Gubišová |
| author_sort | L. Klčová |
| collection | DOAJ |
| description | In the case of poor germination of seed samples and minimal number of seedlings obtained, in vitro methods can be used to revitalise and recover the gene resource. The highest germination of meadow vetchling (Lathyrus pratensis L.) seeds was achieved after scarification with H2SO4 and cultivation in MS medium. The seedlings were used as a material for micropropagation. Regeneration passed through nodal segments cultivated on basal MS medium solidified with a combination of agar and phytagel. This culture medium was also suitable for the plant maintenance. An addition of cytokinin to the induction medium did not support multiplication and growth. In the basal MS medium rooted 72.5% (gene resource 62) or 42.5% (gene resource 28) of shoots. The rooting of gene resource 28 was increased to 63% by the addition of indolylbutyric acid to the culture medium. The regenerated plants were successfully transferred to the soil. This protocol can be used to rescue gene resources of this species. |
| first_indexed | 2024-04-10T08:31:41Z |
| format | Article |
| id | doaj.art-7e3000554ec049b183b1b456668ebd72 |
| institution | Directory Open Access Journal |
| issn | 1212-1975 1805-9325 |
| language | English |
| last_indexed | 2024-04-10T08:31:41Z |
| publishDate | 2003-09-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj.art-7e3000554ec049b183b1b456668ebd722023-02-23T03:28:57ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252003-09-01393848810.17221/3724-CJGPBcjg-200303-0003Utilisation of in vitro Techniques in Rescue of Gene Resources of Meadow Vetchling (Lathyrus pratensis L.)L. Klčová0M. Gubišová1Research Institute of Plant Production, Piešťany, Slovak RepublicResearch Institute of Plant Production, Piešťany, Slovak RepublicIn the case of poor germination of seed samples and minimal number of seedlings obtained, in vitro methods can be used to revitalise and recover the gene resource. The highest germination of meadow vetchling (Lathyrus pratensis L.) seeds was achieved after scarification with H2SO4 and cultivation in MS medium. The seedlings were used as a material for micropropagation. Regeneration passed through nodal segments cultivated on basal MS medium solidified with a combination of agar and phytagel. This culture medium was also suitable for the plant maintenance. An addition of cytokinin to the induction medium did not support multiplication and growth. In the basal MS medium rooted 72.5% (gene resource 62) or 42.5% (gene resource 28) of shoots. The rooting of gene resource 28 was increased to 63% by the addition of indolylbutyric acid to the culture medium. The regenerated plants were successfully transferred to the soil. This protocol can be used to rescue gene resources of this species.https://cjgpb.agriculturejournals.cz/artkey/cjg-200303-0003_utilisation-of-in-vitro-techniques-in-rescue-of-gene-resources-of-meadow-vetchling-lathyrus-pratensis-l.phpmicropropagationin vitro cultivationseed germinationrevitalisationgene resourcesmeadow vetchlinglathyrus pratensis l |
| spellingShingle | L. Klčová M. Gubišová Utilisation of in vitro Techniques in Rescue of Gene Resources of Meadow Vetchling (Lathyrus pratensis L.) Czech Journal of Genetics and Plant Breeding micropropagation in vitro cultivation seed germination revitalisation gene resources meadow vetchling lathyrus pratensis l |
| title | Utilisation of in vitro Techniques in Rescue of Gene Resources of Meadow Vetchling (Lathyrus pratensis L.) |
| title_full | Utilisation of in vitro Techniques in Rescue of Gene Resources of Meadow Vetchling (Lathyrus pratensis L.) |
| title_fullStr | Utilisation of in vitro Techniques in Rescue of Gene Resources of Meadow Vetchling (Lathyrus pratensis L.) |
| title_full_unstemmed | Utilisation of in vitro Techniques in Rescue of Gene Resources of Meadow Vetchling (Lathyrus pratensis L.) |
| title_short | Utilisation of in vitro Techniques in Rescue of Gene Resources of Meadow Vetchling (Lathyrus pratensis L.) |
| title_sort | utilisation of in vitro techniques in rescue of gene resources of meadow vetchling lathyrus pratensis l |
| topic | micropropagation in vitro cultivation seed germination revitalisation gene resources meadow vetchling lathyrus pratensis l |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-200303-0003_utilisation-of-in-vitro-techniques-in-rescue-of-gene-resources-of-meadow-vetchling-lathyrus-pratensis-l.php |
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