Association of Clonal Hematopoiesis of Indeterminate Potential with Inflammatory Gene Expression in Patients with COPD
Chronic obstructive pulmonary disease (COPD) is a disease with an inflammatory phenotype with increasing prevalence in the elderly. Expanded population of mutant blood cells carrying somatic mutations is termed clonal hematopoiesis of indeterminate potential (CHIP). The association between CHIP and...
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MDPI AG
2022-07-01
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| author | Stefan Kuhnert Siavash Mansouri Michael A. Rieger Rajkumar Savai Edibe Avci Gabriela Díaz-Piña Manju Padmasekar Mario Looso Stefan Hadzic Till Acker Stephan Klatt Jochen Wilhelm Ingrid Fleming Natascha Sommer Norbert Weissmann Claus Vogelmeier Robert Bals Andreas Zeiher Stefanie Dimmeler Werner Seeger Soni S. Pullamsetti |
| author_facet | Stefan Kuhnert Siavash Mansouri Michael A. Rieger Rajkumar Savai Edibe Avci Gabriela Díaz-Piña Manju Padmasekar Mario Looso Stefan Hadzic Till Acker Stephan Klatt Jochen Wilhelm Ingrid Fleming Natascha Sommer Norbert Weissmann Claus Vogelmeier Robert Bals Andreas Zeiher Stefanie Dimmeler Werner Seeger Soni S. Pullamsetti |
| author_sort | Stefan Kuhnert |
| collection | DOAJ |
| description | Chronic obstructive pulmonary disease (COPD) is a disease with an inflammatory phenotype with increasing prevalence in the elderly. Expanded population of mutant blood cells carrying somatic mutations is termed clonal hematopoiesis of indeterminate potential (CHIP). The association between CHIP and COPD and its relevant effects on DNA methylation in aging are mainly unknown. Analyzing the deep-targeted amplicon sequencing from 125 COPD patients, we found enhanced incidence of CHIP mutations (~20%) with a predominance of DNMT3A CHIP-mediated hypomethylation of Phospholipase D Family Member 5 (<i>PLD5</i>), which in turn is positively correlated with increased levels of glycerol phosphocholine, pro-inflammatory cytokines, and deteriorating lung function. |
| first_indexed | 2024-03-09T22:00:40Z |
| format | Article |
| id | doaj.art-7e304d43b48b447aafd57d4483baf01e |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T22:00:40Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-7e304d43b48b447aafd57d4483baf01e2023-11-23T19:49:52ZengMDPI AGCells2073-44092022-07-011113212110.3390/cells11132121Association of Clonal Hematopoiesis of Indeterminate Potential with Inflammatory Gene Expression in Patients with COPDStefan Kuhnert0Siavash Mansouri1Michael A. Rieger2Rajkumar Savai3Edibe Avci4Gabriela Díaz-Piña5Manju Padmasekar6Mario Looso7Stefan Hadzic8Till Acker9Stephan Klatt10Jochen Wilhelm11Ingrid Fleming12Natascha Sommer13Norbert Weissmann14Claus Vogelmeier15Robert Bals16Andreas Zeiher17Stefanie Dimmeler18Werner Seeger19Soni S. Pullamsetti20University of Giessen and Marburg Lung Center (UGMLC), German Center for Lung Research (DZL), Cardio-Pulmonary Institute (CPI), Justus Liebig University, 35392 Giessen, GermanyMax Planck Institute for Heart and Lung Research, DZL, CPI, 61231 Bad Nauheim, GermanyDepartment of Medicine, Hematology/Oncology, University Hospital Frankfurt, CPI, Goethe University, 60596 Frankfurt am Main, GermanyUniversity of Giessen and Marburg Lung Center (UGMLC), German Center for Lung Research (DZL), Cardio-Pulmonary Institute (CPI), Justus Liebig University, 35392 Giessen, GermanyMax Planck Institute for Heart and Lung Research, DZL, CPI, 61231 Bad Nauheim, GermanyMax Planck Institute for Heart and Lung Research, DZL, CPI, 61231 Bad Nauheim, GermanyMax Planck Institute for Heart and Lung Research, DZL, CPI, 61231 Bad Nauheim, GermanyMax Planck Institute for Heart and Lung Research, DZL, CPI, 61231 Bad Nauheim, GermanyUniversity of Giessen and Marburg Lung Center (UGMLC), German Center for Lung Research (DZL), Cardio-Pulmonary Institute (CPI), Justus Liebig University, 35392 Giessen, GermanyInstitute for Neuropathology, CPI, Justus Liebig University, 35392 Giessen, GermanyInstitute of Vascular Signalling, Department of Molecular Medicine, CPI, Goethe University, 60596 Frankfurt am Main, GermanyUniversity of Giessen and Marburg Lung Center (UGMLC), German Center for Lung Research (DZL), Cardio-Pulmonary Institute (CPI), Justus Liebig University, 35392 Giessen, GermanyInstitute of Vascular Signalling, Department of Molecular Medicine, CPI, Goethe University, 60596 Frankfurt am Main, GermanyUniversity of Giessen and Marburg Lung Center (UGMLC), German Center for Lung Research (DZL), Cardio-Pulmonary Institute (CPI), Justus Liebig University, 35392 Giessen, GermanyUniversity of Giessen and Marburg Lung Center (UGMLC), German Center for Lung Research (DZL), Cardio-Pulmonary Institute (CPI), Justus Liebig University, 35392 Giessen, GermanyDepartment of Medicine, Pulmonary and Critical Care Medicine, Philipps University of Marburg, DZL, 35043 Marburg, GermanyDepartment of Internal Medicine V-Pulmonology, Allergology and Critical Care Medicine, Saarland University, 66421 Homburg, GermanyDepartment of Medicine, Cardiology, CPI, Goethe University Hospital, 60596 Frankfurt am Main, GermanyInstitute for Cardiovascular Regeneration, CPI, Goethe University, 60596 Frankfurt am Main, GermanyUniversity of Giessen and Marburg Lung Center (UGMLC), German Center for Lung Research (DZL), Cardio-Pulmonary Institute (CPI), Justus Liebig University, 35392 Giessen, GermanyUniversity of Giessen and Marburg Lung Center (UGMLC), German Center for Lung Research (DZL), Cardio-Pulmonary Institute (CPI), Justus Liebig University, 35392 Giessen, GermanyChronic obstructive pulmonary disease (COPD) is a disease with an inflammatory phenotype with increasing prevalence in the elderly. Expanded population of mutant blood cells carrying somatic mutations is termed clonal hematopoiesis of indeterminate potential (CHIP). The association between CHIP and COPD and its relevant effects on DNA methylation in aging are mainly unknown. Analyzing the deep-targeted amplicon sequencing from 125 COPD patients, we found enhanced incidence of CHIP mutations (~20%) with a predominance of DNMT3A CHIP-mediated hypomethylation of Phospholipase D Family Member 5 (<i>PLD5</i>), which in turn is positively correlated with increased levels of glycerol phosphocholine, pro-inflammatory cytokines, and deteriorating lung function.https://www.mdpi.com/2073-4409/11/13/2121clonal hematopoiesisinflammationCOPD |
| spellingShingle | Stefan Kuhnert Siavash Mansouri Michael A. Rieger Rajkumar Savai Edibe Avci Gabriela Díaz-Piña Manju Padmasekar Mario Looso Stefan Hadzic Till Acker Stephan Klatt Jochen Wilhelm Ingrid Fleming Natascha Sommer Norbert Weissmann Claus Vogelmeier Robert Bals Andreas Zeiher Stefanie Dimmeler Werner Seeger Soni S. Pullamsetti Association of Clonal Hematopoiesis of Indeterminate Potential with Inflammatory Gene Expression in Patients with COPD Cells clonal hematopoiesis inflammation COPD |
| title | Association of Clonal Hematopoiesis of Indeterminate Potential with Inflammatory Gene Expression in Patients with COPD |
| title_full | Association of Clonal Hematopoiesis of Indeterminate Potential with Inflammatory Gene Expression in Patients with COPD |
| title_fullStr | Association of Clonal Hematopoiesis of Indeterminate Potential with Inflammatory Gene Expression in Patients with COPD |
| title_full_unstemmed | Association of Clonal Hematopoiesis of Indeterminate Potential with Inflammatory Gene Expression in Patients with COPD |
| title_short | Association of Clonal Hematopoiesis of Indeterminate Potential with Inflammatory Gene Expression in Patients with COPD |
| title_sort | association of clonal hematopoiesis of indeterminate potential with inflammatory gene expression in patients with copd |
| topic | clonal hematopoiesis inflammation COPD |
| url | https://www.mdpi.com/2073-4409/11/13/2121 |
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