Identification of cryodamage on plasma membrane integrity in bull spermatozoa and its relationship with field fertility
The aim of this study was to investigate the effect of cryopreservation on the membrane integrity (MI) in the bull sperm, and determine the relationship between intact cryopreserved sperm and field fertility (FF). The eosin nigrosin exclusion and the hypo-osmotic swelling test were combined in a sin...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universidad del Zulia
2011-10-01
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| Series: | Revista Científica |
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| Online Access: | http://www.produccioncientificaluz.org/index.php/cientifica/article/view/15665 |
| _version_ | 1811163240376303616 |
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| author | Armando Quintero Moreno Jorge Rubio Guillén Decio González Villalobos Juan Carlos Gutiérrez Ninoska Madrid Bury José Julián Garde López Brea |
| author_facet | Armando Quintero Moreno Jorge Rubio Guillén Decio González Villalobos Juan Carlos Gutiérrez Ninoska Madrid Bury José Julián Garde López Brea |
| author_sort | Armando Quintero Moreno |
| collection | DOAJ |
| description | The aim of this study was to investigate the effect of cryopreservation on the membrane integrity (MI) in the bull sperm, and determine the relationship between intact cryopreserved sperm and field fertility (FF). The eosin nigrosin exclusion and the hypo-osmotic swelling test were combined in a single test (HOS-EN test) to identify the spermatozoa with four types of MI. After cryopreservation, there was a marked decline (21.3%) in the percentage of spermatozoa with head membrane intact/ tail membrane intact (Type IV MI), and a significant increase in those with head membrane damaged/ tail membrane damaged (Type I MI) (P |
| first_indexed | 2024-04-10T06:41:53Z |
| format | Article |
| id | doaj.art-7e31fc599a354c328824be55ccbe617a |
| institution | Directory Open Access Journal |
| issn | 0798-2259 2521-9715 |
| language | English |
| last_indexed | 2024-04-10T06:41:53Z |
| publishDate | 2011-10-01 |
| publisher | Universidad del Zulia |
| record_format | Article |
| series | Revista Científica |
| spelling | doaj.art-7e31fc599a354c328824be55ccbe617a2023-02-28T15:24:21ZengUniversidad del ZuliaRevista Científica0798-22592521-97152011-10-01215Identification of cryodamage on plasma membrane integrity in bull spermatozoa and its relationship with field fertilityArmando Quintero Moreno0Jorge Rubio Guillén1Decio González Villalobos2Juan Carlos Gutiérrez3Ninoska Madrid Bury4José Julián Garde López Brea5Universidad del Zulia-VenezuelaUniversidad del Zulia-VenezuelaUniversidad del Zulia-VenezuelaUniversidad del Zulia-VenezuelaUniversidad del Zulia-VenezuelaUniversidad de Castilla-EspañaThe aim of this study was to investigate the effect of cryopreservation on the membrane integrity (MI) in the bull sperm, and determine the relationship between intact cryopreserved sperm and field fertility (FF). The eosin nigrosin exclusion and the hypo-osmotic swelling test were combined in a single test (HOS-EN test) to identify the spermatozoa with four types of MI. After cryopreservation, there was a marked decline (21.3%) in the percentage of spermatozoa with head membrane intact/ tail membrane intact (Type IV MI), and a significant increase in those with head membrane damaged/ tail membrane damaged (Type I MI) (Phttp://www.produccioncientificaluz.org/index.php/cientifica/article/view/15665bull semenHOST testmembrane integrityfield fertility |
| spellingShingle | Armando Quintero Moreno Jorge Rubio Guillén Decio González Villalobos Juan Carlos Gutiérrez Ninoska Madrid Bury José Julián Garde López Brea Identification of cryodamage on plasma membrane integrity in bull spermatozoa and its relationship with field fertility Revista Científica bull semen HOST test membrane integrity field fertility |
| title | Identification of cryodamage on plasma membrane integrity in bull spermatozoa and its relationship with field fertility |
| title_full | Identification of cryodamage on plasma membrane integrity in bull spermatozoa and its relationship with field fertility |
| title_fullStr | Identification of cryodamage on plasma membrane integrity in bull spermatozoa and its relationship with field fertility |
| title_full_unstemmed | Identification of cryodamage on plasma membrane integrity in bull spermatozoa and its relationship with field fertility |
| title_short | Identification of cryodamage on plasma membrane integrity in bull spermatozoa and its relationship with field fertility |
| title_sort | identification of cryodamage on plasma membrane integrity in bull spermatozoa and its relationship with field fertility |
| topic | bull semen HOST test membrane integrity field fertility |
| url | http://www.produccioncientificaluz.org/index.php/cientifica/article/view/15665 |
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