GC-MS Method for Quantification and Pharmacokinetic Study of Four Volatile Compounds in Rat Plasma after Oral Administration of <i>Commiphora myrrh</i> (Nees) Engl. Resin and In Vitro Cytotoxic Evaluation
A rapid, simple, and sensitive gas chromatography–tandem mass spectrometry (GC–MS) method was established and validated for simultaneous determination of four volatile compounds, namely curzerene, methoxyfuranodiene, β-elemene, and α-pinene in rat plasma samples after oral administration of the resi...
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2021-12-01
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| author | Ali S. Alqahtani Rashed N. Herqash Faleh Alqahtani Syed Rizwan Ahamad Fahd A. Nasr Omar M. Noman |
| author_facet | Ali S. Alqahtani Rashed N. Herqash Faleh Alqahtani Syed Rizwan Ahamad Fahd A. Nasr Omar M. Noman |
| author_sort | Ali S. Alqahtani |
| collection | DOAJ |
| description | A rapid, simple, and sensitive gas chromatography–tandem mass spectrometry (GC–MS) method was established and validated for simultaneous determination of four volatile compounds, namely curzerene, methoxyfuranodiene, β-elemene, and α-pinene in rat plasma samples after oral administration of the resin extract of <i>Commiphora</i> <i>myrrh</i> using limonene as an internal standard (IS). Liquid-liquid extraction using hexane and ethyl acetate (1:1) mixture as an extracting agent was used for the samples extraction procedure. The GC–MS system was operated under selective ion monitoring (SIM) mode using Perkin Elmer Elite 5MS column (30 m × 0.25 mm × 0.25 µm film thickness). Specificity, linearity, precision, accuracy, extraction recovery, and stability were used to validate the developed method. The assay showed good linearity (r<sup>2</sup> ≥ 0.998), and the lowest limits of quantification (LLOQ) were 3.97–21.38 ng/mL for the four analytes. This assay was successfully applied to pharmacokinetic studies of the four volatile compounds in rat plasma. The antiproliferative activity of these volatile compounds was evaluated against lung carcinoma (A549) and colon (LoVo) cell lines, were each compound caused variable inhibition on cells proliferation and methoxyfuranodiene exerted the strong antiproliferative activity against both cell line according to IC<sub>50</sub> values. |
| first_indexed | 2024-03-10T03:05:50Z |
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| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-10T03:05:50Z |
| publishDate | 2021-12-01 |
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| spelling | doaj.art-7e32864fecb94c33af4bd0eb074376cd2023-11-23T10:32:39ZengMDPI AGSeparations2297-87392021-12-0181223910.3390/separations8120239GC-MS Method for Quantification and Pharmacokinetic Study of Four Volatile Compounds in Rat Plasma after Oral Administration of <i>Commiphora myrrh</i> (Nees) Engl. Resin and In Vitro Cytotoxic EvaluationAli S. Alqahtani0Rashed N. Herqash1Faleh Alqahtani2Syed Rizwan Ahamad3Fahd A. Nasr4Omar M. Noman5Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaA rapid, simple, and sensitive gas chromatography–tandem mass spectrometry (GC–MS) method was established and validated for simultaneous determination of four volatile compounds, namely curzerene, methoxyfuranodiene, β-elemene, and α-pinene in rat plasma samples after oral administration of the resin extract of <i>Commiphora</i> <i>myrrh</i> using limonene as an internal standard (IS). Liquid-liquid extraction using hexane and ethyl acetate (1:1) mixture as an extracting agent was used for the samples extraction procedure. The GC–MS system was operated under selective ion monitoring (SIM) mode using Perkin Elmer Elite 5MS column (30 m × 0.25 mm × 0.25 µm film thickness). Specificity, linearity, precision, accuracy, extraction recovery, and stability were used to validate the developed method. The assay showed good linearity (r<sup>2</sup> ≥ 0.998), and the lowest limits of quantification (LLOQ) were 3.97–21.38 ng/mL for the four analytes. This assay was successfully applied to pharmacokinetic studies of the four volatile compounds in rat plasma. The antiproliferative activity of these volatile compounds was evaluated against lung carcinoma (A549) and colon (LoVo) cell lines, were each compound caused variable inhibition on cells proliferation and methoxyfuranodiene exerted the strong antiproliferative activity against both cell line according to IC<sub>50</sub> values.https://www.mdpi.com/2297-8739/8/12/239<i>Commiphora myrrh</i>GC–MSplasmapharmacokineticsantiproliferative activity |
| spellingShingle | Ali S. Alqahtani Rashed N. Herqash Faleh Alqahtani Syed Rizwan Ahamad Fahd A. Nasr Omar M. Noman GC-MS Method for Quantification and Pharmacokinetic Study of Four Volatile Compounds in Rat Plasma after Oral Administration of <i>Commiphora myrrh</i> (Nees) Engl. Resin and In Vitro Cytotoxic Evaluation Separations <i>Commiphora myrrh</i> GC–MS plasma pharmacokinetics antiproliferative activity |
| title | GC-MS Method for Quantification and Pharmacokinetic Study of Four Volatile Compounds in Rat Plasma after Oral Administration of <i>Commiphora myrrh</i> (Nees) Engl. Resin and In Vitro Cytotoxic Evaluation |
| title_full | GC-MS Method for Quantification and Pharmacokinetic Study of Four Volatile Compounds in Rat Plasma after Oral Administration of <i>Commiphora myrrh</i> (Nees) Engl. Resin and In Vitro Cytotoxic Evaluation |
| title_fullStr | GC-MS Method for Quantification and Pharmacokinetic Study of Four Volatile Compounds in Rat Plasma after Oral Administration of <i>Commiphora myrrh</i> (Nees) Engl. Resin and In Vitro Cytotoxic Evaluation |
| title_full_unstemmed | GC-MS Method for Quantification and Pharmacokinetic Study of Four Volatile Compounds in Rat Plasma after Oral Administration of <i>Commiphora myrrh</i> (Nees) Engl. Resin and In Vitro Cytotoxic Evaluation |
| title_short | GC-MS Method for Quantification and Pharmacokinetic Study of Four Volatile Compounds in Rat Plasma after Oral Administration of <i>Commiphora myrrh</i> (Nees) Engl. Resin and In Vitro Cytotoxic Evaluation |
| title_sort | gc ms method for quantification and pharmacokinetic study of four volatile compounds in rat plasma after oral administration of i commiphora myrrh i nees engl resin and in vitro cytotoxic evaluation |
| topic | <i>Commiphora myrrh</i> GC–MS plasma pharmacokinetics antiproliferative activity |
| url | https://www.mdpi.com/2297-8739/8/12/239 |
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