The FML (Fucose Mannose Ligand) of Leishmania donovani: a new tool in diagnosis, prognosis, transfusional control and vaccination against human kala-azar
The Fucose-Mannose Ligand (FML) of Leishmania donovani is a complex glycoproteic fraction. Its potential use as a tool for diagnosis of human visceral leishmaniasis was tested with human sera from Natal, Rio Grande do Norte, Brazil. The FML-ELISA test, showed 100% sensitivity and 96% specificity, id...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Medicina Tropical (SBMT)
1996-04-01
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| Series: | Revista da Sociedade Brasileira de Medicina Tropical |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821996000200008&lng=en&tlng=en |
| _version_ | 1818244201472589824 |
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| author | Claris B. Palatnik de Sousa Elza M. Gomes Edilma Paraguai de Souza Wania R. dos Santos Sirley R. de Macedo Linnete V. de Medeiros Kleber Luz |
| author_facet | Claris B. Palatnik de Sousa Elza M. Gomes Edilma Paraguai de Souza Wania R. dos Santos Sirley R. de Macedo Linnete V. de Medeiros Kleber Luz |
| author_sort | Claris B. Palatnik de Sousa |
| collection | DOAJ |
| description | The Fucose-Mannose Ligand (FML) of Leishmania donovani is a complex glycoproteic fraction. Its potential use as a tool for diagnosis of human visceral leishmaniasis was tested with human sera from Natal, Rio Grande do Norte, Brazil. The FML-ELISA test, showed 100% sensitivity and 96% specificity, identifying patients with overt kala-azar (p < 0.001, when compared to normal sera), and subjects with subclinical infection. More than 20% apparently healthy subjects with positive reaction to FML developed overt kala-azar during the following 10 months. In the screening of human blood donnors, a prevalence of 5% of sororeactive subjects was detected, attaining 17% in a single day. The GP36 glycoprotein of FHL is specifically reconized by human kala-azar sera. The immunoprotective effect of FML on experimental L. donovanii infection was tested in swiss albino mice. The protection scheemes included three weekly doses of FML, supplemented or not with saponin by the subcutaneous or intraperitoneal routes and challenge with 2x 10(7) amastigotes of Leishmania donovani. An enhancement of 80.0 % in antibody response (p<0.001) and reduction of 85.5 % parasite liver burden (p<0.001) was detected in animals immunized with FML saponin, unrespectivety of the immunization route. |
| first_indexed | 2024-12-12T14:13:15Z |
| format | Article |
| id | doaj.art-7e34dec8e0fd45f5a4510d5c442b2ccf |
| institution | Directory Open Access Journal |
| issn | 1678-9849 |
| language | English |
| last_indexed | 2024-12-12T14:13:15Z |
| publishDate | 1996-04-01 |
| publisher | Sociedade Brasileira de Medicina Tropical (SBMT) |
| record_format | Article |
| series | Revista da Sociedade Brasileira de Medicina Tropical |
| spelling | doaj.art-7e34dec8e0fd45f5a4510d5c442b2ccf2022-12-22T00:21:59ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical1678-98491996-04-0129215316310.1590/S0037-86821996000200008S0037-86821996000200008The FML (Fucose Mannose Ligand) of Leishmania donovani: a new tool in diagnosis, prognosis, transfusional control and vaccination against human kala-azarClaris B. Palatnik de Sousa0Elza M. Gomes1Edilma Paraguai de Souza2Wania R. dos Santos3Sirley R. de Macedo4Linnete V. de Medeiros5Kleber Luz6Universidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroThe Fucose-Mannose Ligand (FML) of Leishmania donovani is a complex glycoproteic fraction. Its potential use as a tool for diagnosis of human visceral leishmaniasis was tested with human sera from Natal, Rio Grande do Norte, Brazil. The FML-ELISA test, showed 100% sensitivity and 96% specificity, identifying patients with overt kala-azar (p < 0.001, when compared to normal sera), and subjects with subclinical infection. More than 20% apparently healthy subjects with positive reaction to FML developed overt kala-azar during the following 10 months. In the screening of human blood donnors, a prevalence of 5% of sororeactive subjects was detected, attaining 17% in a single day. The GP36 glycoprotein of FHL is specifically reconized by human kala-azar sera. The immunoprotective effect of FML on experimental L. donovanii infection was tested in swiss albino mice. The protection scheemes included three weekly doses of FML, supplemented or not with saponin by the subcutaneous or intraperitoneal routes and challenge with 2x 10(7) amastigotes of Leishmania donovani. An enhancement of 80.0 % in antibody response (p<0.001) and reduction of 85.5 % parasite liver burden (p<0.001) was detected in animals immunized with FML saponin, unrespectivety of the immunization route.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821996000200008&lng=en&tlng=enGlicoconjugadoLeishmania donovaniCalazarTransfusão de sangueDiagnóstico |
| spellingShingle | Claris B. Palatnik de Sousa Elza M. Gomes Edilma Paraguai de Souza Wania R. dos Santos Sirley R. de Macedo Linnete V. de Medeiros Kleber Luz The FML (Fucose Mannose Ligand) of Leishmania donovani: a new tool in diagnosis, prognosis, transfusional control and vaccination against human kala-azar Revista da Sociedade Brasileira de Medicina Tropical Glicoconjugado Leishmania donovani Calazar Transfusão de sangue Diagnóstico |
| title | The FML (Fucose Mannose Ligand) of Leishmania donovani: a new tool in diagnosis, prognosis, transfusional control and vaccination against human kala-azar |
| title_full | The FML (Fucose Mannose Ligand) of Leishmania donovani: a new tool in diagnosis, prognosis, transfusional control and vaccination against human kala-azar |
| title_fullStr | The FML (Fucose Mannose Ligand) of Leishmania donovani: a new tool in diagnosis, prognosis, transfusional control and vaccination against human kala-azar |
| title_full_unstemmed | The FML (Fucose Mannose Ligand) of Leishmania donovani: a new tool in diagnosis, prognosis, transfusional control and vaccination against human kala-azar |
| title_short | The FML (Fucose Mannose Ligand) of Leishmania donovani: a new tool in diagnosis, prognosis, transfusional control and vaccination against human kala-azar |
| title_sort | fml fucose mannose ligand of leishmania donovani a new tool in diagnosis prognosis transfusional control and vaccination against human kala azar |
| topic | Glicoconjugado Leishmania donovani Calazar Transfusão de sangue Diagnóstico |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821996000200008&lng=en&tlng=en |
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