Evaluation of the Effects of Nicotinic Acid and Folic Acid on Morphology, Membrane Integrity and Quality of Sperm Chromatin Concentration in Normozoospermic Men during Cryopreservation.
Background and Aim: Sperm cryopreservation is an important assisted reproductive technique. However, due to increased production of reactive oxygen species and oxidative stress, it can impair sperm function and reduce quality of fertility. The aim of this study was to investigate the effects of nico...
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Kurdistan University of Medical Sciences
2022-05-01
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| Series: | مجله علمی دانشگاه علوم پزشکی کردستان |
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| Online Access: | http://sjku.muk.ac.ir/article-1-5046-en.html |
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| author | Raziah Hashemi Farhad Golshan iranpour Gholam reza dashti Shahla Ishaqi Afsane Jaberi Asl Abol fazl Dashti |
| author_facet | Raziah Hashemi Farhad Golshan iranpour Gholam reza dashti Shahla Ishaqi Afsane Jaberi Asl Abol fazl Dashti |
| author_sort | Raziah Hashemi |
| collection | DOAJ |
| description | Background and Aim: Sperm cryopreservation is an important assisted reproductive technique. However, due to increased production of reactive oxygen species and oxidative stress, it can impair sperm function and reduce quality of fertility. The aim of this study was to investigate the effects of nicotinic acid and folic acid on maintenance of sperm function during cryopreservation by evaluation of sperm parameters and chromatin concentration.
Material and Methods: Thirty samples were collected from normozoospermic men and examined for chromatin quality, viability, membrane integrity, morphology and motility. The samples were frozen and placed in 4 groups: control, folic acid (50 nM), nicotinic acid (10 mM) and a combination of both. After cryopreservation, the four groups were compared with one another in regard to the sperm parameters. Also the sperm parameters were compared before and after freezing in every group. We assessed chromatin quality by TB staining, viability by eosin-nigrosin staining, membrane integrity by hypo osmotic swelling test, and morphology and movement by CASA software.
Results: Before cryopreservation chromatin quality, membrane integrity, normal morphology, sperm motility were lower and immotile sperms were higher in all groups compared to those after cryopreservation (p <0.001). The highest chromatin quality was detected in the folic acid, folic acid + nicotinic acid groups and the lowest chromatin quality was observed in the control group (p</05). The rates of sperm viability and normal morphology were lowest in the control group and highest in the folic acid and other groups (p <0.05). percentage of membrane integrity was highest in the folic acid group followed by nicotinic acid + folic acid, nicotinic acid and control groups, respectively (p <0.05). Folic acid played an important role in maintaining sperm parameters.
Conclusion: Nicotinic acid and folic acid have a positive effect on maintaining sperm function during cryopreservation |
| first_indexed | 2024-04-13T18:29:36Z |
| format | Article |
| id | doaj.art-7e383afb93944981a1e6ec20d8fca189 |
| institution | Directory Open Access Journal |
| issn | 1560-652X 2345-4040 |
| language | fas |
| last_indexed | 2024-04-13T18:29:36Z |
| publishDate | 2022-05-01 |
| publisher | Kurdistan University of Medical Sciences |
| record_format | Article |
| series | مجله علمی دانشگاه علوم پزشکی کردستان |
| spelling | doaj.art-7e383afb93944981a1e6ec20d8fca1892022-12-22T02:35:08ZfasKurdistan University of Medical Sciencesمجله علمی دانشگاه علوم پزشکی کردستان1560-652X2345-40402022-05-012721527Evaluation of the Effects of Nicotinic Acid and Folic Acid on Morphology, Membrane Integrity and Quality of Sperm Chromatin Concentration in Normozoospermic Men during Cryopreservation.Raziah Hashemi0Farhad Golshan iranpour1Gholam reza dashti2Shahla Ishaqi3Afsane Jaberi Asl4Abol fazl Dashti5 Medical doctor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Associated Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Associate Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Laboratory Technician, Shahid behesti hospital, Isfahan University of Medical Sciences, Isfahan, Iran MSc, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Doctor of Veterinary Medicine, School of Veterinary Medicine, Azad University of Shahrekord, Shahrekord, Iran Background and Aim: Sperm cryopreservation is an important assisted reproductive technique. However, due to increased production of reactive oxygen species and oxidative stress, it can impair sperm function and reduce quality of fertility. The aim of this study was to investigate the effects of nicotinic acid and folic acid on maintenance of sperm function during cryopreservation by evaluation of sperm parameters and chromatin concentration. Material and Methods: Thirty samples were collected from normozoospermic men and examined for chromatin quality, viability, membrane integrity, morphology and motility. The samples were frozen and placed in 4 groups: control, folic acid (50 nM), nicotinic acid (10 mM) and a combination of both. After cryopreservation, the four groups were compared with one another in regard to the sperm parameters. Also the sperm parameters were compared before and after freezing in every group. We assessed chromatin quality by TB staining, viability by eosin-nigrosin staining, membrane integrity by hypo osmotic swelling test, and morphology and movement by CASA software. Results: Before cryopreservation chromatin quality, membrane integrity, normal morphology, sperm motility were lower and immotile sperms were higher in all groups compared to those after cryopreservation (p <0.001). The highest chromatin quality was detected in the folic acid, folic acid + nicotinic acid groups and the lowest chromatin quality was observed in the control group (p</05). The rates of sperm viability and normal morphology were lowest in the control group and highest in the folic acid and other groups (p <0.05). percentage of membrane integrity was highest in the folic acid group followed by nicotinic acid + folic acid, nicotinic acid and control groups, respectively (p <0.05). Folic acid played an important role in maintaining sperm parameters. Conclusion: Nicotinic acid and folic acid have a positive effect on maintaining sperm function during cryopreservationhttp://sjku.muk.ac.ir/article-1-5046-en.htmlnicotinic acidfolic acidspermchromatincryopreservation |
| spellingShingle | Raziah Hashemi Farhad Golshan iranpour Gholam reza dashti Shahla Ishaqi Afsane Jaberi Asl Abol fazl Dashti Evaluation of the Effects of Nicotinic Acid and Folic Acid on Morphology, Membrane Integrity and Quality of Sperm Chromatin Concentration in Normozoospermic Men during Cryopreservation. مجله علمی دانشگاه علوم پزشکی کردستان nicotinic acid folic acid sperm chromatin cryopreservation |
| title | Evaluation of the Effects of Nicotinic Acid and Folic Acid on Morphology, Membrane Integrity and Quality of Sperm Chromatin Concentration in Normozoospermic Men during Cryopreservation. |
| title_full | Evaluation of the Effects of Nicotinic Acid and Folic Acid on Morphology, Membrane Integrity and Quality of Sperm Chromatin Concentration in Normozoospermic Men during Cryopreservation. |
| title_fullStr | Evaluation of the Effects of Nicotinic Acid and Folic Acid on Morphology, Membrane Integrity and Quality of Sperm Chromatin Concentration in Normozoospermic Men during Cryopreservation. |
| title_full_unstemmed | Evaluation of the Effects of Nicotinic Acid and Folic Acid on Morphology, Membrane Integrity and Quality of Sperm Chromatin Concentration in Normozoospermic Men during Cryopreservation. |
| title_short | Evaluation of the Effects of Nicotinic Acid and Folic Acid on Morphology, Membrane Integrity and Quality of Sperm Chromatin Concentration in Normozoospermic Men during Cryopreservation. |
| title_sort | evaluation of the effects of nicotinic acid and folic acid on morphology membrane integrity and quality of sperm chromatin concentration in normozoospermic men during cryopreservation |
| topic | nicotinic acid folic acid sperm chromatin cryopreservation |
| url | http://sjku.muk.ac.ir/article-1-5046-en.html |
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