Histomorphometric and immunohistochemical analysis of infectious agents, T-cell subpopulations and inflammatory adhesion molecules in placentas from HIV-seropositive pregnant women
<p>Abstract</p> <p>Background</p> <p>The aim of this study was to compare histomorphometric changes and the results of immunohistochemical tests for VCAM, ICAM-1, CD4 and CD8 in normal placentas from HIV-seropositive pregnant women.</p> <p>Methods</p>...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-10-01
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| Series: | Diagnostic Pathology |
| Subjects: | |
| Online Access: | http://www.diagnosticpathology.org/content/6/1/101 |
| _version_ | 1818039587431251968 |
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| author | Cruz Cristina R Silva Larissa LG Pimentel Camilla R Santos Mônica G Mecatti Maíra C Saito Augusto O Abujamra Karla Souza Camila DA Victor Moreschi N Martins Ana PC Baurakiades Emanuele de Noronha Lucia |
| author_facet | Cruz Cristina R Silva Larissa LG Pimentel Camilla R Santos Mônica G Mecatti Maíra C Saito Augusto O Abujamra Karla Souza Camila DA Victor Moreschi N Martins Ana PC Baurakiades Emanuele de Noronha Lucia |
| author_sort | Cruz Cristina R |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The aim of this study was to compare histomorphometric changes and the results of immunohistochemical tests for VCAM, ICAM-1, CD4 and CD8 in normal placentas from HIV-seropositive pregnant women.</p> <p>Methods</p> <p>Samples of normal placentas were divided into 2 groups: healthy HIV-seronegative pregnant women (control group = C = 60) and HIV-seropositive women (experimental group = E = 57). Conventional histological sections were submitted to morphometric analysis and evaluated in terms of the immunohistochemical expression of ICAM-1, VCAM, CD4 and CD8.</p> <p>Results</p> <p>The villi in group E were smaller than those in group C. The median for the CD8+ T cell count was higher in group E than in group C (p = 0.03). Immunohistochemical expression of ICAM-1 was observed in 57% of the cases in group E, compared with 21% of those in group C (p = 0.001). There was no difference in VCAM expression or CD4+ cell counts between groups and no correlation between the data for antiretroviral therapy and morphometric or immunohistochemical data.</p> <p>Conclusions</p> <p>The morphometric data showed that placentas of HIV-seropositive pregnant women tend to have smaller villi than those of seronegative women. In addition, immunohistochemical testing for infectious agents helped to identify cases that were positive for microorganisms (6/112) that routine pathological examination had failed to detect. The anti-p24 antibody had a limited ability to detect HIV viral protein in this study (2/57). Correlation of immunohistochemical expression of CD8+ T cells and ICAM-1 with the presence of HIV in the placenta revealed that those expressions can act as biomarkers of inflammatory changes. There was no correlation between the data for antiretroviral therapy and morphometric or immunohistochemical data.</p> |
| first_indexed | 2024-12-10T08:01:00Z |
| format | Article |
| id | doaj.art-7e38fefdf26d48a9979300f5c4121b65 |
| institution | Directory Open Access Journal |
| issn | 1746-1596 |
| language | English |
| last_indexed | 2024-12-10T08:01:00Z |
| publishDate | 2011-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Diagnostic Pathology |
| spelling | doaj.art-7e38fefdf26d48a9979300f5c4121b652022-12-22T01:56:48ZengBMCDiagnostic Pathology1746-15962011-10-016110110.1186/1746-1596-6-101Histomorphometric and immunohistochemical analysis of infectious agents, T-cell subpopulations and inflammatory adhesion molecules in placentas from HIV-seropositive pregnant womenCruz Cristina RSilva Larissa LGPimentel Camilla RSantos Mônica GMecatti Maíra CSaito Augusto OAbujamra KarlaSouza Camila DAVictor Moreschi NMartins Ana PCBaurakiades Emanuelede Noronha Lucia<p>Abstract</p> <p>Background</p> <p>The aim of this study was to compare histomorphometric changes and the results of immunohistochemical tests for VCAM, ICAM-1, CD4 and CD8 in normal placentas from HIV-seropositive pregnant women.</p> <p>Methods</p> <p>Samples of normal placentas were divided into 2 groups: healthy HIV-seronegative pregnant women (control group = C = 60) and HIV-seropositive women (experimental group = E = 57). Conventional histological sections were submitted to morphometric analysis and evaluated in terms of the immunohistochemical expression of ICAM-1, VCAM, CD4 and CD8.</p> <p>Results</p> <p>The villi in group E were smaller than those in group C. The median for the CD8+ T cell count was higher in group E than in group C (p = 0.03). Immunohistochemical expression of ICAM-1 was observed in 57% of the cases in group E, compared with 21% of those in group C (p = 0.001). There was no difference in VCAM expression or CD4+ cell counts between groups and no correlation between the data for antiretroviral therapy and morphometric or immunohistochemical data.</p> <p>Conclusions</p> <p>The morphometric data showed that placentas of HIV-seropositive pregnant women tend to have smaller villi than those of seronegative women. In addition, immunohistochemical testing for infectious agents helped to identify cases that were positive for microorganisms (6/112) that routine pathological examination had failed to detect. The anti-p24 antibody had a limited ability to detect HIV viral protein in this study (2/57). Correlation of immunohistochemical expression of CD8+ T cells and ICAM-1 with the presence of HIV in the placenta revealed that those expressions can act as biomarkers of inflammatory changes. There was no correlation between the data for antiretroviral therapy and morphometric or immunohistochemical data.</p>http://www.diagnosticpathology.org/content/6/1/101HIVp24CD4CD8ICAMVCAMplacenta |
| spellingShingle | Cruz Cristina R Silva Larissa LG Pimentel Camilla R Santos Mônica G Mecatti Maíra C Saito Augusto O Abujamra Karla Souza Camila DA Victor Moreschi N Martins Ana PC Baurakiades Emanuele de Noronha Lucia Histomorphometric and immunohistochemical analysis of infectious agents, T-cell subpopulations and inflammatory adhesion molecules in placentas from HIV-seropositive pregnant women Diagnostic Pathology HIV p24 CD4 CD8 ICAM VCAM placenta |
| title | Histomorphometric and immunohistochemical analysis of infectious agents, T-cell subpopulations and inflammatory adhesion molecules in placentas from HIV-seropositive pregnant women |
| title_full | Histomorphometric and immunohistochemical analysis of infectious agents, T-cell subpopulations and inflammatory adhesion molecules in placentas from HIV-seropositive pregnant women |
| title_fullStr | Histomorphometric and immunohistochemical analysis of infectious agents, T-cell subpopulations and inflammatory adhesion molecules in placentas from HIV-seropositive pregnant women |
| title_full_unstemmed | Histomorphometric and immunohistochemical analysis of infectious agents, T-cell subpopulations and inflammatory adhesion molecules in placentas from HIV-seropositive pregnant women |
| title_short | Histomorphometric and immunohistochemical analysis of infectious agents, T-cell subpopulations and inflammatory adhesion molecules in placentas from HIV-seropositive pregnant women |
| title_sort | histomorphometric and immunohistochemical analysis of infectious agents t cell subpopulations and inflammatory adhesion molecules in placentas from hiv seropositive pregnant women |
| topic | HIV p24 CD4 CD8 ICAM VCAM placenta |
| url | http://www.diagnosticpathology.org/content/6/1/101 |
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