A Comparative Histopathological Investigation of the Effects of ZnO/ Chitosan Nanocomposites and ZnO Nanoparticles on Thioacetamide-induced Nephrophaty in Rats
Background and purpose: This study compared the effects of intraperitoneal injections of ZnO/chitosan nanocomposite and ZnO nanoparticles on histopathology of kidney in thioacetamide-intoxicated rats. Materials and methods: Twenty-eight male rats were randomly allocated into a healthy control group...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2019-09-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
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| Online Access: | http://jmums.mazums.ac.ir/article-1-12682-en.html |
| _version_ | 1797943925026062336 |
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| author | Mohammad Reza Hajinezhad Abbas Jamshidian Alireza Samzadeh kermani |
| author_facet | Mohammad Reza Hajinezhad Abbas Jamshidian Alireza Samzadeh kermani |
| author_sort | Mohammad Reza Hajinezhad |
| collection | DOAJ |
| description | Background and purpose: This study compared the effects of intraperitoneal injections of ZnO/chitosan nanocomposite and ZnO nanoparticles on histopathology of kidney in thioacetamide-intoxicated rats.
Materials and methods: Twenty-eight male rats were randomly allocated into a healthy control group (negative control), positive control group (thioacetamide-intoxicated rats), thioacetamide-intoxicated + ZnO nanoparticles, and thioacetamide- iontoxicated + ZnO/chitosan nanocomposite. Nephropathy was induced by intraperitoneal injection of thioacetamide (50 mg/kg IP) three times (24h interval). The treatment groups were treated with ZnO nanocomposites (5mg/kg) and ZnO nanoparticles (5mg/kg) intraperitoneally for four weeks. Then, serum BUN and creatinine levels were measured. Afterwards, the rats were euthanized and kidney samples were separated for histological examinations.
Results: There were significant increases in serum BUN and creatinine levels in thioacetamide-intoxicated rats compared to those in the control group (P<0.05). Compared to the control group, ZnO nanoparticles –treated group was found with significantly higher serum BUN and creatinine levels (P<0.05). There was no significant difference between the rats treated with ZnO/chitosan nanocomposites and the positive control group in BUN and creatinine levels (P>0.05). In histological analysis, tubular necrosis and glomerular fibrosis were observed in treatment groups.
Conclusion: This study showed that ZnO nanoparticles can increase nephrotoxicity induced by thioacetamide, but, ZnO/chitosan nanocomposite had no effect on thioacetamide induced nephrotoxicity. |
| first_indexed | 2024-04-10T20:30:48Z |
| format | Article |
| id | doaj.art-7e3949e24dc843b4ba192fb2726accfb |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-04-10T20:30:48Z |
| publishDate | 2019-09-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-7e3949e24dc843b4ba192fb2726accfb2023-01-25T06:18:58ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792019-09-0129176189194A Comparative Histopathological Investigation of the Effects of ZnO/ Chitosan Nanocomposites and ZnO Nanoparticles on Thioacetamide-induced Nephrophaty in RatsMohammad Reza Hajinezhad0Abbas Jamshidian1Alireza Samzadeh kermani2 Associate Professor, Department of Basic Science, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran Assistant Professor, Department of Pthobiology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran Associate Professor, Department of Chemistry, Faculty of Basic Sciences, University of Zabol, Zabol, Iran Background and purpose: This study compared the effects of intraperitoneal injections of ZnO/chitosan nanocomposite and ZnO nanoparticles on histopathology of kidney in thioacetamide-intoxicated rats. Materials and methods: Twenty-eight male rats were randomly allocated into a healthy control group (negative control), positive control group (thioacetamide-intoxicated rats), thioacetamide-intoxicated + ZnO nanoparticles, and thioacetamide- iontoxicated + ZnO/chitosan nanocomposite. Nephropathy was induced by intraperitoneal injection of thioacetamide (50 mg/kg IP) three times (24h interval). The treatment groups were treated with ZnO nanocomposites (5mg/kg) and ZnO nanoparticles (5mg/kg) intraperitoneally for four weeks. Then, serum BUN and creatinine levels were measured. Afterwards, the rats were euthanized and kidney samples were separated for histological examinations. Results: There were significant increases in serum BUN and creatinine levels in thioacetamide-intoxicated rats compared to those in the control group (P<0.05). Compared to the control group, ZnO nanoparticles –treated group was found with significantly higher serum BUN and creatinine levels (P<0.05). There was no significant difference between the rats treated with ZnO/chitosan nanocomposites and the positive control group in BUN and creatinine levels (P>0.05). In histological analysis, tubular necrosis and glomerular fibrosis were observed in treatment groups. Conclusion: This study showed that ZnO nanoparticles can increase nephrotoxicity induced by thioacetamide, but, ZnO/chitosan nanocomposite had no effect on thioacetamide induced nephrotoxicity.http://jmums.mazums.ac.ir/article-1-12682-en.htmlliverzno/chitosan nanoparticleszno nanocompositeskidneyrat |
| spellingShingle | Mohammad Reza Hajinezhad Abbas Jamshidian Alireza Samzadeh kermani A Comparative Histopathological Investigation of the Effects of ZnO/ Chitosan Nanocomposites and ZnO Nanoparticles on Thioacetamide-induced Nephrophaty in Rats Journal of Mazandaran University of Medical Sciences liver zno/chitosan nanoparticles zno nanocomposites kidney rat |
| title | A Comparative Histopathological Investigation of the Effects of ZnO/ Chitosan Nanocomposites and ZnO Nanoparticles on Thioacetamide-induced Nephrophaty in Rats |
| title_full | A Comparative Histopathological Investigation of the Effects of ZnO/ Chitosan Nanocomposites and ZnO Nanoparticles on Thioacetamide-induced Nephrophaty in Rats |
| title_fullStr | A Comparative Histopathological Investigation of the Effects of ZnO/ Chitosan Nanocomposites and ZnO Nanoparticles on Thioacetamide-induced Nephrophaty in Rats |
| title_full_unstemmed | A Comparative Histopathological Investigation of the Effects of ZnO/ Chitosan Nanocomposites and ZnO Nanoparticles on Thioacetamide-induced Nephrophaty in Rats |
| title_short | A Comparative Histopathological Investigation of the Effects of ZnO/ Chitosan Nanocomposites and ZnO Nanoparticles on Thioacetamide-induced Nephrophaty in Rats |
| title_sort | comparative histopathological investigation of the effects of zno chitosan nanocomposites and zno nanoparticles on thioacetamide induced nephrophaty in rats |
| topic | liver zno/chitosan nanoparticles zno nanocomposites kidney rat |
| url | http://jmums.mazums.ac.ir/article-1-12682-en.html |
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