New Interactors of the Truncated EBNA-LP Protein Identified by Mass Spectrometry in P3HR1 Burkitt’s Lymphoma Cells
The Epstein-Barr virus nuclear antigen leader protein (EBNA-LP) acts as a co-activator of EBNA-2, a transcriptional activator essential for Epstein-Barr virus (EBV)-induced B-cell transformation. Burkitt’s lymphoma (BL) cells harboring a mutant EBV strain that lacks both the EBNA-2 gene and 3′ exons...
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MDPI AG
2018-01-01
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| Series: | Cancers |
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| Online Access: | http://www.mdpi.com/2072-6694/10/1/12 |
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| author | Sonia Chelouah Emilie Cochet Sophie Couvé Sandy Balkaran Aude Robert Evelyne May Vasily Ogryzko Joëlle Wiels |
| author_facet | Sonia Chelouah Emilie Cochet Sophie Couvé Sandy Balkaran Aude Robert Evelyne May Vasily Ogryzko Joëlle Wiels |
| author_sort | Sonia Chelouah |
| collection | DOAJ |
| description | The Epstein-Barr virus nuclear antigen leader protein (EBNA-LP) acts as a co-activator of EBNA-2, a transcriptional activator essential for Epstein-Barr virus (EBV)-induced B-cell transformation. Burkitt’s lymphoma (BL) cells harboring a mutant EBV strain that lacks both the EBNA-2 gene and 3′ exons of EBNA-LP express Y1Y2-truncated isoforms of EBNA-LP (tEBNA-LP) and better resist apoptosis than if infected with the wild-type virus. In such BL cells, tEBNA-LP interacts with the protein phosphatase 2A (PP2A) catalytic subunit (PP2A C), and this interaction likely plays a role in resistance to apoptosis. Here, 28 cellular and four viral proteins have been identified by mass spectrometry as further possible interactors of tEBNA-LP. Three interactions were confirmed by immunoprecipitation and Western blotting, namely with the A structural subunit of PP2A (PP2A A), the structure-specific recognition protein 1 (SSRP1, a component of the facilitate chromatin transcription (FACT) complex), and a new form of the transcription factor EC (TFEC). Thus, tEBNA-LP appears to be involved not only in cell resistance to apoptosis through its interaction with two PP2A subunits, but also in other processes where its ability to co-activate transcriptional regulators could be important. |
| first_indexed | 2024-03-12T10:36:41Z |
| format | Article |
| id | doaj.art-7e3b9524108041b18ef46001f909d7de |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-12T10:36:41Z |
| publishDate | 2018-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-7e3b9524108041b18ef46001f909d7de2023-09-02T08:36:32ZengMDPI AGCancers2072-66942018-01-011011210.3390/cancers10010012cancers10010012New Interactors of the Truncated EBNA-LP Protein Identified by Mass Spectrometry in P3HR1 Burkitt’s Lymphoma CellsSonia Chelouah0Emilie Cochet1Sophie Couvé2Sandy Balkaran3Aude Robert4Evelyne May5Vasily Ogryzko6Joëlle Wiels7UMR 8126 CNRS, Univ. Paris-Sud, Université Paris-Saclay, Institut Gustave Roussy, F-94805 Villejuif, FranceProteomic Platform, Institut Gustave Roussy, Villejuif F-94805, FranceLaboratoire de Génétique Oncologique, Ecole Pratique des Hautes Etudes, F-75014 Paris, FranceUMR 8126 CNRS, Univ. Paris-Sud, Université Paris-Saclay, Institut Gustave Roussy, F-94805 Villejuif, FranceUMR 8126 CNRS, Univ. Paris-Sud, Université Paris-Saclay, Institut Gustave Roussy, F-94805 Villejuif, FranceUMR 8126 CNRS, Univ. Paris-Sud, Université Paris-Saclay, Institut Gustave Roussy, F-94805 Villejuif, FranceUMR 8126 CNRS, Univ. Paris-Sud, Université Paris-Saclay, Institut Gustave Roussy, F-94805 Villejuif, FranceUMR 8126 CNRS, Univ. Paris-Sud, Université Paris-Saclay, Institut Gustave Roussy, F-94805 Villejuif, FranceThe Epstein-Barr virus nuclear antigen leader protein (EBNA-LP) acts as a co-activator of EBNA-2, a transcriptional activator essential for Epstein-Barr virus (EBV)-induced B-cell transformation. Burkitt’s lymphoma (BL) cells harboring a mutant EBV strain that lacks both the EBNA-2 gene and 3′ exons of EBNA-LP express Y1Y2-truncated isoforms of EBNA-LP (tEBNA-LP) and better resist apoptosis than if infected with the wild-type virus. In such BL cells, tEBNA-LP interacts with the protein phosphatase 2A (PP2A) catalytic subunit (PP2A C), and this interaction likely plays a role in resistance to apoptosis. Here, 28 cellular and four viral proteins have been identified by mass spectrometry as further possible interactors of tEBNA-LP. Three interactions were confirmed by immunoprecipitation and Western blotting, namely with the A structural subunit of PP2A (PP2A A), the structure-specific recognition protein 1 (SSRP1, a component of the facilitate chromatin transcription (FACT) complex), and a new form of the transcription factor EC (TFEC). Thus, tEBNA-LP appears to be involved not only in cell resistance to apoptosis through its interaction with two PP2A subunits, but also in other processes where its ability to co-activate transcriptional regulators could be important.http://www.mdpi.com/2072-6694/10/1/12EBVWp-restricted latencymass spectrometryEBNA-LPPP2ASSRP1TFEC |
| spellingShingle | Sonia Chelouah Emilie Cochet Sophie Couvé Sandy Balkaran Aude Robert Evelyne May Vasily Ogryzko Joëlle Wiels New Interactors of the Truncated EBNA-LP Protein Identified by Mass Spectrometry in P3HR1 Burkitt’s Lymphoma Cells Cancers EBV Wp-restricted latency mass spectrometry EBNA-LP PP2A SSRP1 TFEC |
| title | New Interactors of the Truncated EBNA-LP Protein Identified by Mass Spectrometry in P3HR1 Burkitt’s Lymphoma Cells |
| title_full | New Interactors of the Truncated EBNA-LP Protein Identified by Mass Spectrometry in P3HR1 Burkitt’s Lymphoma Cells |
| title_fullStr | New Interactors of the Truncated EBNA-LP Protein Identified by Mass Spectrometry in P3HR1 Burkitt’s Lymphoma Cells |
| title_full_unstemmed | New Interactors of the Truncated EBNA-LP Protein Identified by Mass Spectrometry in P3HR1 Burkitt’s Lymphoma Cells |
| title_short | New Interactors of the Truncated EBNA-LP Protein Identified by Mass Spectrometry in P3HR1 Burkitt’s Lymphoma Cells |
| title_sort | new interactors of the truncated ebna lp protein identified by mass spectrometry in p3hr1 burkitt s lymphoma cells |
| topic | EBV Wp-restricted latency mass spectrometry EBNA-LP PP2A SSRP1 TFEC |
| url | http://www.mdpi.com/2072-6694/10/1/12 |
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