New Look at RSV Infection: Tissue Clearing and 3D Imaging of the Entire Mouse Lung at Cellular Resolution
Background: Respiratory Syncytial Virus (RSV) is the major cause of severe acute respiratory tract illness in young children worldwide and a main pathogen for the elderly and immune-compromised people. In the absence of vaccines or effective treatments, a better characterization of the pathogenesis...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/13/2/201 |
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| author | Maxence Frétaud Delphyne Descamps Daphné Laubreton Marie-Anne Rameix-Welti Jean-François Eléouët Thibaut Larcher Marie Galloux Christelle Langevin |
| author_facet | Maxence Frétaud Delphyne Descamps Daphné Laubreton Marie-Anne Rameix-Welti Jean-François Eléouët Thibaut Larcher Marie Galloux Christelle Langevin |
| author_sort | Maxence Frétaud |
| collection | DOAJ |
| description | Background: Respiratory Syncytial Virus (RSV) is the major cause of severe acute respiratory tract illness in young children worldwide and a main pathogen for the elderly and immune-compromised people. In the absence of vaccines or effective treatments, a better characterization of the pathogenesis of RSV infection is required. To date, the pathophysiology of the disease and its diagnosis has mostly relied on chest X-ray and genome detection in nasopharyngeal swabs. The development of new imaging approaches is instrumental to further the description of RSV spread, virus–host interactions and related acute respiratory disease, at the level of the entire lung. Methods: By combining tissue clearing, 3D microscopy and image processing, we developed a novel visualization tool of RSV infection in undissected mouse lungs. Results: Whole tissue analysis allowed the identification of infected cell subtypes, based on both morphological traits and position within the cellular network. Furthermore, 3D imaging was also valuable to detect the cytoplasmic viral factories, also called inclusion bodies, a hallmark of RSV infection. Conclusions: Whole lung clearing and 3D deep imaging represents an unprecedented visualization method of infected lungs to allow insight into RSV pathophysiology and improve the 2D histology analyses. |
| first_indexed | 2024-03-09T03:22:34Z |
| format | Article |
| id | doaj.art-7e3cd78b4d734052aebb1f6b47b560c3 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T03:22:34Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-7e3cd78b4d734052aebb1f6b47b560c32023-12-03T15:07:38ZengMDPI AGViruses1999-49152021-01-0113220110.3390/v13020201New Look at RSV Infection: Tissue Clearing and 3D Imaging of the Entire Mouse Lung at Cellular ResolutionMaxence Frétaud0Delphyne Descamps1Daphné Laubreton2Marie-Anne Rameix-Welti3Jean-François Eléouët4Thibaut Larcher5Marie Galloux6Christelle Langevin7Université Paris-Saclay, INRAE, UVSQ, VIM, 78350 Jouy-en-Josas, FranceUniversité Paris-Saclay, INRAE, UVSQ, VIM, 78350 Jouy-en-Josas, FranceUniversité Paris-Saclay, INRAE, UVSQ, VIM, 78350 Jouy-en-Josas, FranceUniversité Paris-Saclay, INSERM, Université de Versailles St. Quentin, UMR 1173 (2I), 78000 Versailles, FranceUniversité Paris-Saclay, INRAE, UVSQ, VIM, 78350 Jouy-en-Josas, FranceINRAE, Oniris, UMR 703 APEX, 44307 Nantes, FranceUniversité Paris-Saclay, INRAE, UVSQ, VIM, 78350 Jouy-en-Josas, FranceUniversité Paris-Saclay, INRAE, UVSQ, VIM, 78350 Jouy-en-Josas, FranceBackground: Respiratory Syncytial Virus (RSV) is the major cause of severe acute respiratory tract illness in young children worldwide and a main pathogen for the elderly and immune-compromised people. In the absence of vaccines or effective treatments, a better characterization of the pathogenesis of RSV infection is required. To date, the pathophysiology of the disease and its diagnosis has mostly relied on chest X-ray and genome detection in nasopharyngeal swabs. The development of new imaging approaches is instrumental to further the description of RSV spread, virus–host interactions and related acute respiratory disease, at the level of the entire lung. Methods: By combining tissue clearing, 3D microscopy and image processing, we developed a novel visualization tool of RSV infection in undissected mouse lungs. Results: Whole tissue analysis allowed the identification of infected cell subtypes, based on both morphological traits and position within the cellular network. Furthermore, 3D imaging was also valuable to detect the cytoplasmic viral factories, also called inclusion bodies, a hallmark of RSV infection. Conclusions: Whole lung clearing and 3D deep imaging represents an unprecedented visualization method of infected lungs to allow insight into RSV pathophysiology and improve the 2D histology analyses.https://www.mdpi.com/1999-4915/13/2/201RSV infectiontissue clearing3D imaging of lungsinclusion bodiesviral pathophysiologyRSV tropism |
| spellingShingle | Maxence Frétaud Delphyne Descamps Daphné Laubreton Marie-Anne Rameix-Welti Jean-François Eléouët Thibaut Larcher Marie Galloux Christelle Langevin New Look at RSV Infection: Tissue Clearing and 3D Imaging of the Entire Mouse Lung at Cellular Resolution Viruses RSV infection tissue clearing 3D imaging of lungs inclusion bodies viral pathophysiology RSV tropism |
| title | New Look at RSV Infection: Tissue Clearing and 3D Imaging of the Entire Mouse Lung at Cellular Resolution |
| title_full | New Look at RSV Infection: Tissue Clearing and 3D Imaging of the Entire Mouse Lung at Cellular Resolution |
| title_fullStr | New Look at RSV Infection: Tissue Clearing and 3D Imaging of the Entire Mouse Lung at Cellular Resolution |
| title_full_unstemmed | New Look at RSV Infection: Tissue Clearing and 3D Imaging of the Entire Mouse Lung at Cellular Resolution |
| title_short | New Look at RSV Infection: Tissue Clearing and 3D Imaging of the Entire Mouse Lung at Cellular Resolution |
| title_sort | new look at rsv infection tissue clearing and 3d imaging of the entire mouse lung at cellular resolution |
| topic | RSV infection tissue clearing 3D imaging of lungs inclusion bodies viral pathophysiology RSV tropism |
| url | https://www.mdpi.com/1999-4915/13/2/201 |
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