Advances in Label-Free Detections for Nanofluidic Analytical Devices
Nanofluidics, a discipline of science and engineering of fluids confined to structures at the 1–1000 nm scale, has experienced significant growth over the past decade. Nanofluidics have offered fascinating platforms for chemical and biological analyses by exploiting the unique characteristics of liq...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/11/10/885 |
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| author | Thu Hac Huong Le Hisashi Shimizu Kyojiro Morikawa |
| author_facet | Thu Hac Huong Le Hisashi Shimizu Kyojiro Morikawa |
| author_sort | Thu Hac Huong Le |
| collection | DOAJ |
| description | Nanofluidics, a discipline of science and engineering of fluids confined to structures at the 1–1000 nm scale, has experienced significant growth over the past decade. Nanofluidics have offered fascinating platforms for chemical and biological analyses by exploiting the unique characteristics of liquids and molecules confined in nanospaces; however, the difficulty to detect molecules in extremely small spaces hampers the practical applications of nanofluidic devices. Laser-induced fluorescence microscopy with single-molecule sensitivity has been so far a major detection method in nanofluidics, but issues arising from labeling and photobleaching limit its application. Recently, numerous label-free detection methods have been developed to identify and determine the number of molecules, as well as provide chemical, conformational, and kinetic information of molecules. This review focuses on label-free detection techniques designed for nanofluidics; these techniques are divided into two groups: optical and electrical/electrochemical detection methods. In this review, we discuss on the developed nanofluidic device architectures, elucidate the mechanisms by which the utilization of nanofluidics in manipulating molecules and controlling light–matter interactions enhances the capabilities of biological and chemical analyses, and highlight new research directions in the field of detections in nanofluidics. |
| first_indexed | 2024-03-10T16:05:44Z |
| format | Article |
| id | doaj.art-7e3ec31a214c40cb83e8e1e6bce1e131 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T16:05:44Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-7e3ec31a214c40cb83e8e1e6bce1e1312023-11-20T14:52:17ZengMDPI AGMicromachines2072-666X2020-09-01111088510.3390/mi11100885Advances in Label-Free Detections for Nanofluidic Analytical DevicesThu Hac Huong Le0Hisashi Shimizu1Kyojiro Morikawa2Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656, JapanCollaborative Research Organization for Micro and Nano Multifunctional Devices (NMfD), The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656, JapanCollaborative Research Organization for Micro and Nano Multifunctional Devices (NMfD), The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656, JapanNanofluidics, a discipline of science and engineering of fluids confined to structures at the 1–1000 nm scale, has experienced significant growth over the past decade. Nanofluidics have offered fascinating platforms for chemical and biological analyses by exploiting the unique characteristics of liquids and molecules confined in nanospaces; however, the difficulty to detect molecules in extremely small spaces hampers the practical applications of nanofluidic devices. Laser-induced fluorescence microscopy with single-molecule sensitivity has been so far a major detection method in nanofluidics, but issues arising from labeling and photobleaching limit its application. Recently, numerous label-free detection methods have been developed to identify and determine the number of molecules, as well as provide chemical, conformational, and kinetic information of molecules. This review focuses on label-free detection techniques designed for nanofluidics; these techniques are divided into two groups: optical and electrical/electrochemical detection methods. In this review, we discuss on the developed nanofluidic device architectures, elucidate the mechanisms by which the utilization of nanofluidics in manipulating molecules and controlling light–matter interactions enhances the capabilities of biological and chemical analyses, and highlight new research directions in the field of detections in nanofluidics.https://www.mdpi.com/2072-666X/11/10/885nanofluidicsnanofluidic analytical devicelabel-free detectionmicroTASlab-on-a-chip |
| spellingShingle | Thu Hac Huong Le Hisashi Shimizu Kyojiro Morikawa Advances in Label-Free Detections for Nanofluidic Analytical Devices Micromachines nanofluidics nanofluidic analytical device label-free detection microTAS lab-on-a-chip |
| title | Advances in Label-Free Detections for Nanofluidic Analytical Devices |
| title_full | Advances in Label-Free Detections for Nanofluidic Analytical Devices |
| title_fullStr | Advances in Label-Free Detections for Nanofluidic Analytical Devices |
| title_full_unstemmed | Advances in Label-Free Detections for Nanofluidic Analytical Devices |
| title_short | Advances in Label-Free Detections for Nanofluidic Analytical Devices |
| title_sort | advances in label free detections for nanofluidic analytical devices |
| topic | nanofluidics nanofluidic analytical device label-free detection microTAS lab-on-a-chip |
| url | https://www.mdpi.com/2072-666X/11/10/885 |
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