Evaluation of inorganic and organic bone components after application of an apatite-coated Al2O3 implants as scaffolds for bone repair
The aim of this work was to study the influence of uncoated and apatite-coated Al2O3 implants on bone regeneration after 30 days of surgery in New Zealand white rabbits. Twelve samples of Al2O3 implants were prepared and half of them (n = 6) were apatite-coated by the modified biomimetic method. Thr...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2014-01-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300004&tlng=en |
| _version_ | 1798026169305530368 |
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| author | Antonio Luiz Martins Maia Filho Fabrício Pires de Moura do Amaral Airton Abrahão Martin Luís Eduardo Silva Soares |
| author_facet | Antonio Luiz Martins Maia Filho Fabrício Pires de Moura do Amaral Airton Abrahão Martin Luís Eduardo Silva Soares |
| author_sort | Antonio Luiz Martins Maia Filho |
| collection | DOAJ |
| description | The aim of this work was to study the influence of uncoated and apatite-coated Al2O3 implants on bone regeneration after 30 days of surgery in New Zealand white rabbits. Twelve samples of Al2O3 implants were prepared and half of them (n = 6) were apatite-coated by the modified biomimetic method. Three experimental groups were tested as Group C - control, surgery procedure without cerami c implant, Group Ce - uncoated Al 2O3 implants (n = 6) and Group CeHA - apatite-coated Al2O3 implants (n = 6). The mineralization of the Al2O3 implants was analyzed in bone fragments using FT-Raman spectroscopy. Raman peaks at 959 cm-1 and 2940 cm-1 evaluated the inorganic and organic bone content, respectively. In vivo citotoxicity was analyzed using micronucleus test. Inorganic and organic content were higher in CeHA samples than in Ce and C (CeHA > Ce > C). FT-Raman spectroscopy showed that the higher the deposition of the organic matrix, more mineralization occurred. The micronucleus test showed that the uncoated and apatite-coated Al2O3 implants were non-cytotoxic and safe to in vivo applications. |
| first_indexed | 2024-04-11T18:31:03Z |
| format | Article |
| id | doaj.art-7e457681a31c4f93b97d0232a3136b06 |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-04-11T18:31:03Z |
| publishDate | 2014-01-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-7e457681a31c4f93b97d0232a3136b062022-12-22T04:09:27ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242014-01-0157333433910.1590/S1516-89132014005000002Evaluation of inorganic and organic bone components after application of an apatite-coated Al2O3 implants as scaffolds for bone repairAntonio Luiz Martins Maia Filho0Fabrício Pires de Moura do Amaral1Airton Abrahão Martin2Luís Eduardo Silva Soares3Faculdade Santo AgostinhoFaculdade Integral DiferencialUniversidade do Vale do ParaíbaUniversidade do Vale do ParaíbaThe aim of this work was to study the influence of uncoated and apatite-coated Al2O3 implants on bone regeneration after 30 days of surgery in New Zealand white rabbits. Twelve samples of Al2O3 implants were prepared and half of them (n = 6) were apatite-coated by the modified biomimetic method. Three experimental groups were tested as Group C - control, surgery procedure without cerami c implant, Group Ce - uncoated Al 2O3 implants (n = 6) and Group CeHA - apatite-coated Al2O3 implants (n = 6). The mineralization of the Al2O3 implants was analyzed in bone fragments using FT-Raman spectroscopy. Raman peaks at 959 cm-1 and 2940 cm-1 evaluated the inorganic and organic bone content, respectively. In vivo citotoxicity was analyzed using micronucleus test. Inorganic and organic content were higher in CeHA samples than in Ce and C (CeHA > Ce > C). FT-Raman spectroscopy showed that the higher the deposition of the organic matrix, more mineralization occurred. The micronucleus test showed that the uncoated and apatite-coated Al2O3 implants were non-cytotoxic and safe to in vivo applications.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300004&tlng=enBone regenerationAlumina foamsApatite coatingFT-Raman spectroscopyMicronucleus test |
| spellingShingle | Antonio Luiz Martins Maia Filho Fabrício Pires de Moura do Amaral Airton Abrahão Martin Luís Eduardo Silva Soares Evaluation of inorganic and organic bone components after application of an apatite-coated Al2O3 implants as scaffolds for bone repair Brazilian Archives of Biology and Technology Bone regeneration Alumina foams Apatite coating FT-Raman spectroscopy Micronucleus test |
| title | Evaluation of inorganic and organic bone components after application of an apatite-coated Al2O3 implants as scaffolds for bone repair |
| title_full | Evaluation of inorganic and organic bone components after application of an apatite-coated Al2O3 implants as scaffolds for bone repair |
| title_fullStr | Evaluation of inorganic and organic bone components after application of an apatite-coated Al2O3 implants as scaffolds for bone repair |
| title_full_unstemmed | Evaluation of inorganic and organic bone components after application of an apatite-coated Al2O3 implants as scaffolds for bone repair |
| title_short | Evaluation of inorganic and organic bone components after application of an apatite-coated Al2O3 implants as scaffolds for bone repair |
| title_sort | evaluation of inorganic and organic bone components after application of an apatite coated al2o3 implants as scaffolds for bone repair |
| topic | Bone regeneration Alumina foams Apatite coating FT-Raman spectroscopy Micronucleus test |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300004&tlng=en |
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