Evaluation of lung flute in sputum samples for molecular analysis of lung cancer
Abstract Background Molecular analysis of sputum provides a promising approach for lung cancer diagnosis, yet is limited by the difficulty in collecting the specimens from individuals who can't spontaneously expectorate sputum. Lung Flute is a small self‐powered audio device that can induce spu...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-12-01
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| Series: | Clinical and Translational Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/2001-1326-2-15 |
| _version_ | 1828860874900635648 |
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| author | Nigar Anjuman Ning Li Maria Guarnera Sanford A Stass Feng Jiang |
| author_facet | Nigar Anjuman Ning Li Maria Guarnera Sanford A Stass Feng Jiang |
| author_sort | Nigar Anjuman |
| collection | DOAJ |
| description | Abstract Background Molecular analysis of sputum provides a promising approach for lung cancer diagnosis, yet is limited by the difficulty in collecting the specimens from individuals who can't spontaneously expectorate sputum. Lung Flute is a small self‐powered audio device that can induce sputum by generating sound waves and vibrating in the airways of the lungs. Here we propose to evaluate the usefulness of Lung Flute for sputum sampling to assist diagnosis of lung cancer. Methods Forty‐three stage I lung cancer patients and 47 cancer‐free individuals who couldn't spontaneously cough sputum were instructed to use Lung Flute for sputum sampling. Expressions of two microRNAs, miRs‐31 and 210, were determined in the specimens by qRT‐PCR. The results were compared with sputum cytology. Results Sputum was easily collected from 39 of 43 (90.7%) lung cancer patients and 42 of 47 (89.4%) controls with volume ranges from 1 to 5 ml (median, 2.6 ml). The specimens had less than 4% oral squamous cells, indicating that sputum was obtained from low respiratory tract. Expressions of miRs‐31 and 210 in sputum were considerably higher in cancer patients than cancer‐free individuals (8.990 vs. 4.514; 0.6847 vs. 0.3317; all P <0.001). Combined use of the two miRNAs produced a significantly higher sensitivity (61.5% vs. 35.9%, P = 0.002) and a slightly lower specificity (90.5% vs. 95.2%, p = 0.03) compared with cytology for lung cancer diagnosis. Conclusion Lung Flute could potentially be useful in convenient and efficient collection of sputum for molecular diagnosis of lung cancer. |
| first_indexed | 2024-12-13T02:49:06Z |
| format | Article |
| id | doaj.art-7e4671784c004af28c0c71521d1b6bfc |
| institution | Directory Open Access Journal |
| issn | 2001-1326 |
| language | English |
| last_indexed | 2024-12-13T02:49:06Z |
| publishDate | 2013-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Translational Medicine |
| spelling | doaj.art-7e4671784c004af28c0c71521d1b6bfc2022-12-22T00:02:06ZengWileyClinical and Translational Medicine2001-13262013-12-0121n/an/a10.1186/2001-1326-2-15Evaluation of lung flute in sputum samples for molecular analysis of lung cancerNigar Anjuman0Ning Li1Maria Guarnera2Sanford A Stass3Feng Jiang4Departments of PathologyUniversity of Maryland School of Medicine10 South Pine Street, MSTF 7th floor21201‐1192BaltimoreMDUSADepartments of PathologyUniversity of Maryland School of Medicine10 South Pine Street, MSTF 7th floor21201‐1192BaltimoreMDUSADepartments of PathologyUniversity of Maryland School of Medicine10 South Pine Street, MSTF 7th floor21201‐1192BaltimoreMDUSADepartments of PathologyUniversity of Maryland School of Medicine10 South Pine Street, MSTF 7th floor21201‐1192BaltimoreMDUSADepartments of PathologyUniversity of Maryland School of Medicine10 South Pine Street, MSTF 7th floor21201‐1192BaltimoreMDUSAAbstract Background Molecular analysis of sputum provides a promising approach for lung cancer diagnosis, yet is limited by the difficulty in collecting the specimens from individuals who can't spontaneously expectorate sputum. Lung Flute is a small self‐powered audio device that can induce sputum by generating sound waves and vibrating in the airways of the lungs. Here we propose to evaluate the usefulness of Lung Flute for sputum sampling to assist diagnosis of lung cancer. Methods Forty‐three stage I lung cancer patients and 47 cancer‐free individuals who couldn't spontaneously cough sputum were instructed to use Lung Flute for sputum sampling. Expressions of two microRNAs, miRs‐31 and 210, were determined in the specimens by qRT‐PCR. The results were compared with sputum cytology. Results Sputum was easily collected from 39 of 43 (90.7%) lung cancer patients and 42 of 47 (89.4%) controls with volume ranges from 1 to 5 ml (median, 2.6 ml). The specimens had less than 4% oral squamous cells, indicating that sputum was obtained from low respiratory tract. Expressions of miRs‐31 and 210 in sputum were considerably higher in cancer patients than cancer‐free individuals (8.990 vs. 4.514; 0.6847 vs. 0.3317; all P <0.001). Combined use of the two miRNAs produced a significantly higher sensitivity (61.5% vs. 35.9%, P = 0.002) and a slightly lower specificity (90.5% vs. 95.2%, p = 0.03) compared with cytology for lung cancer diagnosis. Conclusion Lung Flute could potentially be useful in convenient and efficient collection of sputum for molecular diagnosis of lung cancer.https://doi.org/10.1186/2001-1326-2-15Lung fluteSputummicroRNAsLung cancerDiagnosis |
| spellingShingle | Nigar Anjuman Ning Li Maria Guarnera Sanford A Stass Feng Jiang Evaluation of lung flute in sputum samples for molecular analysis of lung cancer Clinical and Translational Medicine Lung flute Sputum microRNAs Lung cancer Diagnosis |
| title | Evaluation of lung flute in sputum samples for molecular analysis of lung cancer |
| title_full | Evaluation of lung flute in sputum samples for molecular analysis of lung cancer |
| title_fullStr | Evaluation of lung flute in sputum samples for molecular analysis of lung cancer |
| title_full_unstemmed | Evaluation of lung flute in sputum samples for molecular analysis of lung cancer |
| title_short | Evaluation of lung flute in sputum samples for molecular analysis of lung cancer |
| title_sort | evaluation of lung flute in sputum samples for molecular analysis of lung cancer |
| topic | Lung flute Sputum microRNAs Lung cancer Diagnosis |
| url | https://doi.org/10.1186/2001-1326-2-15 |
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