<b>Dynamics in global DNA methylation and endogenous polyamine levels during protocorm-like bodies induction of <i>Cattleya tigrina</i> A. Richard
Cattleya tigrina is endemic to the Atlantic forest biome and classified as vulnerable in the Red Book of Brazilian Flora. In vitro techniques comprise valuable tools for the conservation of endangered plant species. The aim of the present study was to evaluate the morphological features, global DNA...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Maringá
2017-11-01
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| Series: | Acta Scientiarum: Biological Sciences |
| Subjects: | |
| Online Access: | https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/36656 |
| _version_ | 1811326335479447552 |
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| author | Vivian Almeida Hugo Pacheco de Freitas Fraga Bruno Bachiega Navarro Miguel Pedro Guerra Rosete Pescador |
| author_facet | Vivian Almeida Hugo Pacheco de Freitas Fraga Bruno Bachiega Navarro Miguel Pedro Guerra Rosete Pescador |
| author_sort | Vivian Almeida |
| collection | DOAJ |
| description | Cattleya tigrina is endemic to the Atlantic forest biome and classified as vulnerable in the Red Book of Brazilian Flora. In vitro techniques comprise valuable tools for the conservation of endangered plant species. The aim of the present study was to evaluate the morphological features, global DNA methylation levels and free polyamines during protocorm-like bodies (PLBs) induction of C. tigrina. Along with that, an efficient protocol for in vitro propagation of this species is proposed. The first evidences of PLBs induction in C. tigrina occurred at seven days in culture, starting from the basal portion of the leaf abaxial surface. A hypomethylation marked the beginning of cell differentiation, followed by an increased global DNA methylation at 35 days in culture, coinciding with a subtle change in the structures morphogenetic development. During PLBs induction, putrescine exhibited higher levels as compared to spermidine and spermine, and apparently presents a major role during the PLBs induction in C. tigrina. Due to the apparent secondary PLBs formation, this protocol can represent a highly efficient method for in vitro propagation of this species.
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| first_indexed | 2024-04-13T14:48:05Z |
| format | Article |
| id | doaj.art-7e46747718cb4434bbc9a5707fa56e2f |
| institution | Directory Open Access Journal |
| issn | 1679-9283 1807-863X |
| language | English |
| last_indexed | 2024-04-13T14:48:05Z |
| publishDate | 2017-11-01 |
| publisher | Universidade Estadual de Maringá |
| record_format | Article |
| series | Acta Scientiarum: Biological Sciences |
| spelling | doaj.art-7e46747718cb4434bbc9a5707fa56e2f2022-12-22T02:42:41ZengUniversidade Estadual de MaringáActa Scientiarum: Biological Sciences1679-92831807-863X2017-11-0139410.4025/actascibiolsci.v39i4.3665617229<b>Dynamics in global DNA methylation and endogenous polyamine levels during protocorm-like bodies induction of <i>Cattleya tigrina</i> A. RichardVivian Almeida0Hugo Pacheco de Freitas Fraga1Bruno Bachiega Navarro2Miguel Pedro Guerra3Rosete Pescador4Universidade Federal de Santa CatarinaUniversidade Federal do ParanáUniversidade Federal de Santa CatarinaUniversidade Federal de Santa Catarina / National Research Council of ItalyUniversidade Federal de Santa CatarinaCattleya tigrina is endemic to the Atlantic forest biome and classified as vulnerable in the Red Book of Brazilian Flora. In vitro techniques comprise valuable tools for the conservation of endangered plant species. The aim of the present study was to evaluate the morphological features, global DNA methylation levels and free polyamines during protocorm-like bodies (PLBs) induction of C. tigrina. Along with that, an efficient protocol for in vitro propagation of this species is proposed. The first evidences of PLBs induction in C. tigrina occurred at seven days in culture, starting from the basal portion of the leaf abaxial surface. A hypomethylation marked the beginning of cell differentiation, followed by an increased global DNA methylation at 35 days in culture, coinciding with a subtle change in the structures morphogenetic development. During PLBs induction, putrescine exhibited higher levels as compared to spermidine and spermine, and apparently presents a major role during the PLBs induction in C. tigrina. Due to the apparent secondary PLBs formation, this protocol can represent a highly efficient method for in vitro propagation of this species. https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/36656orchidsmicropropagationin vitro cultureepigeneticsPLBs. |
| spellingShingle | Vivian Almeida Hugo Pacheco de Freitas Fraga Bruno Bachiega Navarro Miguel Pedro Guerra Rosete Pescador <b>Dynamics in global DNA methylation and endogenous polyamine levels during protocorm-like bodies induction of <i>Cattleya tigrina</i> A. Richard Acta Scientiarum: Biological Sciences orchids micropropagation in vitro culture epigenetics PLBs. |
| title | <b>Dynamics in global DNA methylation and endogenous polyamine levels during protocorm-like bodies induction of <i>Cattleya tigrina</i> A. Richard |
| title_full | <b>Dynamics in global DNA methylation and endogenous polyamine levels during protocorm-like bodies induction of <i>Cattleya tigrina</i> A. Richard |
| title_fullStr | <b>Dynamics in global DNA methylation and endogenous polyamine levels during protocorm-like bodies induction of <i>Cattleya tigrina</i> A. Richard |
| title_full_unstemmed | <b>Dynamics in global DNA methylation and endogenous polyamine levels during protocorm-like bodies induction of <i>Cattleya tigrina</i> A. Richard |
| title_short | <b>Dynamics in global DNA methylation and endogenous polyamine levels during protocorm-like bodies induction of <i>Cattleya tigrina</i> A. Richard |
| title_sort | b dynamics in global dna methylation and endogenous polyamine levels during protocorm like bodies induction of i cattleya tigrina i a richard |
| topic | orchids micropropagation in vitro culture epigenetics PLBs. |
| url | https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/36656 |
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