Phosphoglycerate dehydrogenase positively regulates the proliferation of chicken muscle cells
ABSTRACT: Phosphoglycerate dehydrogenase (PHGDH) is the rate-limiting enzyme in the serine synthesis pathway. However, the regulatory role of PHGDH in muscle development is unclear. We report that the expression of PHGDH increased significantly during proliferation of chicken skeletal muscle satelli...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-05-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579122001134 |
| _version_ | 1828342709475082240 |
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| author | Han Wang Moran Hu Zhaoxue Ding Xiaolong Zhou Songbai Yang Zhonghao Shen Feifei Yan Ayong Zhao |
| author_facet | Han Wang Moran Hu Zhaoxue Ding Xiaolong Zhou Songbai Yang Zhonghao Shen Feifei Yan Ayong Zhao |
| author_sort | Han Wang |
| collection | DOAJ |
| description | ABSTRACT: Phosphoglycerate dehydrogenase (PHGDH) is the rate-limiting enzyme in the serine synthesis pathway. However, the regulatory role of PHGDH in muscle development is unclear. We report that the expression of PHGDH increased significantly during proliferation of chicken skeletal muscle satellite cells. Knockdown of PHGDH by an siRNA suppressed myoblast proliferation, whereas overexpression of PHGDH enhanced muscle cell proliferation. Furthermore, PHGDH promoted the expression of Forkhead box protein M1 (FoxM1). Knockdown of FoxM1 by an siRNA attenuated the proliferation of chicken muscle cells, whereas its overexpression significantly promoted proliferation. Additionally, siRNA-PHGDH inhibited pcDNA3.1-FoxM1-induced FoxM1 expression in chicken muscle cells. Moreover, PHGDH inhibition overcame the stimulation by pcDNA3.1-FoxM1 of cell cycle-related gene expression. We propose that PHGDH accelerates chicken muscle cell proliferation by increasing FoxM1 expression. |
| first_indexed | 2024-04-13T23:33:30Z |
| format | Article |
| id | doaj.art-7e4810e830664fb49e91f5cffa64ccaf |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-13T23:33:30Z |
| publishDate | 2022-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-7e4810e830664fb49e91f5cffa64ccaf2022-12-22T02:24:49ZengElsevierPoultry Science0032-57912022-05-011015101805Phosphoglycerate dehydrogenase positively regulates the proliferation of chicken muscle cellsHan Wang0Moran Hu1Zhaoxue Ding2Xiaolong Zhou3Songbai Yang4Zhonghao Shen5Feifei Yan6Ayong Zhao7Key Laboratory of Applied Technology on Green-Eco Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Zhejiang 311300, ChinaKey Laboratory of Applied Technology on Green-Eco Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Zhejiang 311300, ChinaKey Laboratory of Applied Technology on Green-Eco Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Zhejiang 311300, ChinaKey Laboratory of Applied Technology on Green-Eco Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Zhejiang 311300, ChinaKey Laboratory of Applied Technology on Green-Eco Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Zhejiang 311300, ChinaKey Laboratory of Applied Technology on Green-Eco Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Zhejiang 311300, ChinaKey Laboratory of Applied Technology on Green-Eco Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Zhejiang 311300, ChinaCorresponding author:; Key Laboratory of Applied Technology on Green-Eco Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Zhejiang 311300, ChinaABSTRACT: Phosphoglycerate dehydrogenase (PHGDH) is the rate-limiting enzyme in the serine synthesis pathway. However, the regulatory role of PHGDH in muscle development is unclear. We report that the expression of PHGDH increased significantly during proliferation of chicken skeletal muscle satellite cells. Knockdown of PHGDH by an siRNA suppressed myoblast proliferation, whereas overexpression of PHGDH enhanced muscle cell proliferation. Furthermore, PHGDH promoted the expression of Forkhead box protein M1 (FoxM1). Knockdown of FoxM1 by an siRNA attenuated the proliferation of chicken muscle cells, whereas its overexpression significantly promoted proliferation. Additionally, siRNA-PHGDH inhibited pcDNA3.1-FoxM1-induced FoxM1 expression in chicken muscle cells. Moreover, PHGDH inhibition overcame the stimulation by pcDNA3.1-FoxM1 of cell cycle-related gene expression. We propose that PHGDH accelerates chicken muscle cell proliferation by increasing FoxM1 expression.http://www.sciencedirect.com/science/article/pii/S0032579122001134chickenskeletal musclecell proliferationPHGDHFoxM1 |
| spellingShingle | Han Wang Moran Hu Zhaoxue Ding Xiaolong Zhou Songbai Yang Zhonghao Shen Feifei Yan Ayong Zhao Phosphoglycerate dehydrogenase positively regulates the proliferation of chicken muscle cells Poultry Science chicken skeletal muscle cell proliferation PHGDH FoxM1 |
| title | Phosphoglycerate dehydrogenase positively regulates the proliferation of chicken muscle cells |
| title_full | Phosphoglycerate dehydrogenase positively regulates the proliferation of chicken muscle cells |
| title_fullStr | Phosphoglycerate dehydrogenase positively regulates the proliferation of chicken muscle cells |
| title_full_unstemmed | Phosphoglycerate dehydrogenase positively regulates the proliferation of chicken muscle cells |
| title_short | Phosphoglycerate dehydrogenase positively regulates the proliferation of chicken muscle cells |
| title_sort | phosphoglycerate dehydrogenase positively regulates the proliferation of chicken muscle cells |
| topic | chicken skeletal muscle cell proliferation PHGDH FoxM1 |
| url | http://www.sciencedirect.com/science/article/pii/S0032579122001134 |
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