Biofilm-forming antimicrobial-resistant pathogenic Escherichia coli: A one health challenge in Northeast India
This study aimed to investigate the prevalence of Shiga toxin-producing Escherichia coli (STEC), Enteropathogenic E. coli (EPEC), and Enterotoxigenic E. coli (ETEC) in common food animals (cattle, goats, and pigs) reared by tribal communities and smallholder farmers in Northeast India. The isolates...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023072675 |
| _version_ | 1797669778980077568 |
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| author | A. Arun Prince Milton K. Srinivas Vanita Lyngdoh Aleimo G. Momin Naphisabet Lapang G. Bhuvana Priya Sandeep Ghatak R.K. Sanjukta Arnab Sen Samir Das |
| author_facet | A. Arun Prince Milton K. Srinivas Vanita Lyngdoh Aleimo G. Momin Naphisabet Lapang G. Bhuvana Priya Sandeep Ghatak R.K. Sanjukta Arnab Sen Samir Das |
| author_sort | A. Arun Prince Milton |
| collection | DOAJ |
| description | This study aimed to investigate the prevalence of Shiga toxin-producing Escherichia coli (STEC), Enteropathogenic E. coli (EPEC), and Enterotoxigenic E. coli (ETEC) in common food animals (cattle, goats, and pigs) reared by tribal communities and smallholder farmers in Northeast India. The isolates were characterized for the presence of virulence genes, extended-spectrum beta-lactamases (ESBL) production, antimicrobial resistance, and biofilm production, and the results were statistically interpreted. In pathotyping 141 E. coli isolates, 10 (7.09%, 95% CI: 3.45%–12.66%) were identified as STEC, 2 (1.42%, 95% CI: 0.17%–5.03%) as atypical-EPEC, and 1 (0.71%, 95% CI: 0.02%–3.89%) as typical-EPEC. None of the isolates were classified as ETEC. Additionally, using the phenotypic combination disc method (ceftazidime with and without clavulanic acid), six isolates (46.1%, 95% CI: 19.22%–74.87%) were determined to be ESBL producers. Among the STEC/EPEC strains, eleven (84.6%, 95% CI: 54.55%–98.08%) and one (7.7%, 95% CI: 0.19%–36.03%) strains were capable of producing strong or moderate biofilms, respectively. PFGE analysis revealed indistinguishable patterns for certain isolates, suggesting clonal relationships. These findings highlight the potential role of food animals reared by tribal communities and smallholder farmers as reservoirs of virulent biofilm-forming E. coli pathotypes, with implications for food contamination and zoonotic infections. Therefore, monitoring these pathogens in food animals is crucial for optimizing public health through one health strategy. |
| first_indexed | 2024-03-11T20:48:31Z |
| format | Article |
| id | doaj.art-7e48e7ace85a49e1a18315fa62c05bed |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-11T20:48:31Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-7e48e7ace85a49e1a18315fa62c05bed2023-10-01T06:02:25ZengElsevierHeliyon2405-84402023-09-0199e20059Biofilm-forming antimicrobial-resistant pathogenic Escherichia coli: A one health challenge in Northeast IndiaA. Arun Prince Milton0K. Srinivas1Vanita Lyngdoh2Aleimo G. Momin3Naphisabet Lapang4G. Bhuvana Priya5Sandeep Ghatak6R.K. Sanjukta7Arnab Sen8Samir Das9Division of Animal and Fisheries Sciences, ICAR Research Complex for Northeastern Hill Region, Umiam, Meghalaya, India; Corresponding author.Division of Animal and Fisheries Sciences, ICAR Research Complex for Northeastern Hill Region, Umiam, Meghalaya, IndiaDivision of Animal and Fisheries Sciences, ICAR Research Complex for Northeastern Hill Region, Umiam, Meghalaya, IndiaDivision of Animal and Fisheries Sciences, ICAR Research Complex for Northeastern Hill Region, Umiam, Meghalaya, IndiaDivision of Animal and Fisheries Sciences, ICAR Research Complex for Northeastern Hill Region, Umiam, Meghalaya, IndiaCollege of Agriculture, Central Agricultural University (Imphal), Kyrdemkulai, Meghalaya, IndiaDivision of Animal and Fisheries Sciences, ICAR Research Complex for Northeastern Hill Region, Umiam, Meghalaya, IndiaDivision of Animal and Fisheries Sciences, ICAR Research Complex for Northeastern Hill Region, Umiam, Meghalaya, IndiaDivision of Animal and Fisheries Sciences, ICAR Research Complex for Northeastern Hill Region, Umiam, Meghalaya, IndiaDivision of Animal and Fisheries Sciences, ICAR Research Complex for Northeastern Hill Region, Umiam, Meghalaya, India; Corresponding author.This study aimed to investigate the prevalence of Shiga toxin-producing Escherichia coli (STEC), Enteropathogenic E. coli (EPEC), and Enterotoxigenic E. coli (ETEC) in common food animals (cattle, goats, and pigs) reared by tribal communities and smallholder farmers in Northeast India. The isolates were characterized for the presence of virulence genes, extended-spectrum beta-lactamases (ESBL) production, antimicrobial resistance, and biofilm production, and the results were statistically interpreted. In pathotyping 141 E. coli isolates, 10 (7.09%, 95% CI: 3.45%–12.66%) were identified as STEC, 2 (1.42%, 95% CI: 0.17%–5.03%) as atypical-EPEC, and 1 (0.71%, 95% CI: 0.02%–3.89%) as typical-EPEC. None of the isolates were classified as ETEC. Additionally, using the phenotypic combination disc method (ceftazidime with and without clavulanic acid), six isolates (46.1%, 95% CI: 19.22%–74.87%) were determined to be ESBL producers. Among the STEC/EPEC strains, eleven (84.6%, 95% CI: 54.55%–98.08%) and one (7.7%, 95% CI: 0.19%–36.03%) strains were capable of producing strong or moderate biofilms, respectively. PFGE analysis revealed indistinguishable patterns for certain isolates, suggesting clonal relationships. These findings highlight the potential role of food animals reared by tribal communities and smallholder farmers as reservoirs of virulent biofilm-forming E. coli pathotypes, with implications for food contamination and zoonotic infections. Therefore, monitoring these pathogens in food animals is crucial for optimizing public health through one health strategy.http://www.sciencedirect.com/science/article/pii/S2405844023072675E. coliSTECEPECBiofilmESBLPFGE |
| spellingShingle | A. Arun Prince Milton K. Srinivas Vanita Lyngdoh Aleimo G. Momin Naphisabet Lapang G. Bhuvana Priya Sandeep Ghatak R.K. Sanjukta Arnab Sen Samir Das Biofilm-forming antimicrobial-resistant pathogenic Escherichia coli: A one health challenge in Northeast India Heliyon E. coli STEC EPEC Biofilm ESBL PFGE |
| title | Biofilm-forming antimicrobial-resistant pathogenic Escherichia coli: A one health challenge in Northeast India |
| title_full | Biofilm-forming antimicrobial-resistant pathogenic Escherichia coli: A one health challenge in Northeast India |
| title_fullStr | Biofilm-forming antimicrobial-resistant pathogenic Escherichia coli: A one health challenge in Northeast India |
| title_full_unstemmed | Biofilm-forming antimicrobial-resistant pathogenic Escherichia coli: A one health challenge in Northeast India |
| title_short | Biofilm-forming antimicrobial-resistant pathogenic Escherichia coli: A one health challenge in Northeast India |
| title_sort | biofilm forming antimicrobial resistant pathogenic escherichia coli a one health challenge in northeast india |
| topic | E. coli STEC EPEC Biofilm ESBL PFGE |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023072675 |
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