Detection of metallo-β-lactamases-encoding genes among clinical isolates of Pseudomonas aeruginosa in a tertiary care hospital, Kathmandu, Nepal
Abstract Objectives This study was carried out to determine the prevalence of metallo-β-lactamases (MBLs) producing Pseudomonas aeruginosa in imipenem-nonsusceptible isolates and to detect MBL-encoding genes among MBLs-positive isolates. Results Metallo-β-lactamases production was detected in 68.6%...
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BMC
2017-12-01
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| Series: | BMC Research Notes |
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| Online Access: | http://link.springer.com/article/10.1186/s13104-017-3068-9 |
| _version_ | 1819160307090587648 |
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| author | Mahesh Acharya Prabhu R. Joshi Kamal Thapa Rajan Aryal Trishna Kakshapati Supriya Sharma |
| author_facet | Mahesh Acharya Prabhu R. Joshi Kamal Thapa Rajan Aryal Trishna Kakshapati Supriya Sharma |
| author_sort | Mahesh Acharya |
| collection | DOAJ |
| description | Abstract Objectives This study was carried out to determine the prevalence of metallo-β-lactamases (MBLs) producing Pseudomonas aeruginosa in imipenem-nonsusceptible isolates and to detect MBL-encoding genes among MBLs-positive isolates. Results Metallo-β-lactamases production was detected in 68.6% isolates of P. aeruginosa with reduced susceptibility to imipenem. The bla VIM-2 gene was detected in 75% isolates and bla IMP-1 was detected in 25% isolates. All MBLs-positive isolates were multidrug resistant with a high level of resistance to imipenem (MIC 16 to ≥ 32 µg/ml), meropenem (MIC 16 to ≥ 32 µg/ml), and ceftazidime (MIC 64 to ≥ 512 µg/ml). All MBL-positive isolates were susceptible (MIC ≤ 2 µg/ml) to colistin. We found high prevalence of MBL-producing P. aeruginosa. To our knowledge this is the first report of detection of bla VIM-2 and bla IMP-1 in P. aeruginosa from Nepal. This indicates the need for awareness to prevent the spreading of these resistant isolates in hospital setting. |
| first_indexed | 2024-12-22T16:54:22Z |
| format | Article |
| id | doaj.art-7e4d5ea4b7c3490ab22b8db146ebbb7f |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-22T16:54:22Z |
| publishDate | 2017-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-7e4d5ea4b7c3490ab22b8db146ebbb7f2022-12-21T18:19:30ZengBMCBMC Research Notes1756-05002017-12-011011510.1186/s13104-017-3068-9Detection of metallo-β-lactamases-encoding genes among clinical isolates of Pseudomonas aeruginosa in a tertiary care hospital, Kathmandu, NepalMahesh Acharya0Prabhu R. Joshi1Kamal Thapa2Rajan Aryal3Trishna Kakshapati4Supriya Sharma5Central Department of Microbiology, Tribhuvan UniversityCentral Department of Microbiology, Tribhuvan UniversityKathmandu College of Science and Technology, Tribhuvan UniversityKantipur College of Medical Sciences, Tribhuvan UniversityAnnapurna Neurological Institute and Allied SciencesCentral Department of Microbiology, Tribhuvan UniversityAbstract Objectives This study was carried out to determine the prevalence of metallo-β-lactamases (MBLs) producing Pseudomonas aeruginosa in imipenem-nonsusceptible isolates and to detect MBL-encoding genes among MBLs-positive isolates. Results Metallo-β-lactamases production was detected in 68.6% isolates of P. aeruginosa with reduced susceptibility to imipenem. The bla VIM-2 gene was detected in 75% isolates and bla IMP-1 was detected in 25% isolates. All MBLs-positive isolates were multidrug resistant with a high level of resistance to imipenem (MIC 16 to ≥ 32 µg/ml), meropenem (MIC 16 to ≥ 32 µg/ml), and ceftazidime (MIC 64 to ≥ 512 µg/ml). All MBL-positive isolates were susceptible (MIC ≤ 2 µg/ml) to colistin. We found high prevalence of MBL-producing P. aeruginosa. To our knowledge this is the first report of detection of bla VIM-2 and bla IMP-1 in P. aeruginosa from Nepal. This indicates the need for awareness to prevent the spreading of these resistant isolates in hospital setting.http://link.springer.com/article/10.1186/s13104-017-3068-9Metallo-β-lactamasesImipenem-nonsusceptiblebla VIM-2bla IMP-1 |
| spellingShingle | Mahesh Acharya Prabhu R. Joshi Kamal Thapa Rajan Aryal Trishna Kakshapati Supriya Sharma Detection of metallo-β-lactamases-encoding genes among clinical isolates of Pseudomonas aeruginosa in a tertiary care hospital, Kathmandu, Nepal BMC Research Notes Metallo-β-lactamases Imipenem-nonsusceptible bla VIM-2 bla IMP-1 |
| title | Detection of metallo-β-lactamases-encoding genes among clinical isolates of Pseudomonas aeruginosa in a tertiary care hospital, Kathmandu, Nepal |
| title_full | Detection of metallo-β-lactamases-encoding genes among clinical isolates of Pseudomonas aeruginosa in a tertiary care hospital, Kathmandu, Nepal |
| title_fullStr | Detection of metallo-β-lactamases-encoding genes among clinical isolates of Pseudomonas aeruginosa in a tertiary care hospital, Kathmandu, Nepal |
| title_full_unstemmed | Detection of metallo-β-lactamases-encoding genes among clinical isolates of Pseudomonas aeruginosa in a tertiary care hospital, Kathmandu, Nepal |
| title_short | Detection of metallo-β-lactamases-encoding genes among clinical isolates of Pseudomonas aeruginosa in a tertiary care hospital, Kathmandu, Nepal |
| title_sort | detection of metallo β lactamases encoding genes among clinical isolates of pseudomonas aeruginosa in a tertiary care hospital kathmandu nepal |
| topic | Metallo-β-lactamases Imipenem-nonsusceptible bla VIM-2 bla IMP-1 |
| url | http://link.springer.com/article/10.1186/s13104-017-3068-9 |
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