Effects of the kinetic pattern of dietary glucose release on nitrogen utilization, the portal amino acid profile, and nutrient transporter expression in intestinal enterocytes in piglets
Abstract Background Promoting the synchronization of glucose and amino acid release in the digestive tract of pigs could effectively improve dietary nitrogen utilization. The rational allocation of dietary starch sources and the exploration of appropriate dietary glucose release kinetics may promote...
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| Format: | Article |
| Language: | English |
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BMC
2024-03-01
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| Series: | Journal of Animal Science and Biotechnology |
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| Online Access: | https://doi.org/10.1186/s40104-024-01000-z |
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| author | Zexi Li Yunfei Li Yufei Zhao Guifu Wang Rujie Liu Yue Li Qamar Aftab Zewei Sun Qingzhen Zhong |
| author_facet | Zexi Li Yunfei Li Yufei Zhao Guifu Wang Rujie Liu Yue Li Qamar Aftab Zewei Sun Qingzhen Zhong |
| author_sort | Zexi Li |
| collection | DOAJ |
| description | Abstract Background Promoting the synchronization of glucose and amino acid release in the digestive tract of pigs could effectively improve dietary nitrogen utilization. The rational allocation of dietary starch sources and the exploration of appropriate dietary glucose release kinetics may promote the dynamic balance of dietary glucose and amino acid supplies. However, research on the effects of diets with different glucose release kinetic profiles on amino acid absorption and portal amino acid appearance in piglets is limited. This study aimed to investigate the effects of the kinetic pattern of dietary glucose release on nitrogen utilization, the portal amino acid profile, and nutrient transporter expression in intestinal enterocytes in piglets. Methods Sixty-four barrows (15.00 ± 1.12 kg) were randomly allotted to 4 groups and fed diets formulated with starch from corn, corn/barley, corn/sorghum, or corn/cassava combinations (diets were coded A, B, C, or D respectively). Protein retention, the concentrations of portal amino acid and glucose, and the relative expression of amino acid and glucose transporter mRNAs were investigated. In vitro digestion was used to compare the dietary glucose release profiles. Results Four piglet diets with different glucose release kinetics were constructed by adjusting starch sources. The in vivo appearance dynamics of portal glucose were consistent with those of in vitro dietary glucose release kinetics. Total nitrogen excretion was reduced in the piglets in group B, while apparent nitrogen digestibility and nitrogen retention increased (P < 0.05). Regardless of the time (2 h or 4 h after morning feeding), the portal total free amino acids content and contents of some individual amino acids (Thr, Glu, Gly, Ala, and Ile) of the piglets in group B were significantly higher than those in groups A, C, and D (P < 0.05). Cluster analysis showed that different glucose release kinetic patterns resulted in different portal amino acid patterns in piglets, which decreased gradually with the extension of feeding time. The portal His/Phe, Pro/Glu, Leu/Val, Lys/Met, Tyr/Ile and Ala/Gly appeared higher similarity among the diet treatments. In the anterior jejunum, the glucose transporter SGLT1 was significantly positively correlated with the amino acid transporters B 0 AT1, EAAC1, and CAT1. Conclusions Rational allocation of starch resources could regulate dietary glucose release kinetics. In the present study, group B (corn/barley) diet exhibited a better glucose release kinetic pattern than the other groups, which could affect the portal amino acid contents and patterns by regulating the expression of amino acid transporters in the small intestine, thereby promoting nitrogen deposition in the body, and improving the utilization efficiency of dietary nitrogen. |
| first_indexed | 2024-04-24T19:53:55Z |
| format | Article |
| id | doaj.art-7e51e7c6c5474e65b9d9066fe7836c44 |
| institution | Directory Open Access Journal |
| issn | 2049-1891 |
| language | English |
| last_indexed | 2025-03-21T12:04:57Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
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| series | Journal of Animal Science and Biotechnology |
| spelling | doaj.art-7e51e7c6c5474e65b9d9066fe7836c442024-06-30T11:28:50ZengBMCJournal of Animal Science and Biotechnology2049-18912024-03-0115111610.1186/s40104-024-01000-zEffects of the kinetic pattern of dietary glucose release on nitrogen utilization, the portal amino acid profile, and nutrient transporter expression in intestinal enterocytes in pigletsZexi Li0Yunfei Li1Yufei Zhao2Guifu Wang3Rujie Liu4Yue Li5Qamar Aftab6Zewei Sun7Qingzhen Zhong8Jilin Province Key Laboratory of Animal Nutrition and Feed Science, College of Animal Science and Technology, Key Lab of Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural UniversityJilin Province Key Laboratory of Animal Nutrition and Feed Science, College of Animal Science and Technology, Key Lab of Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural UniversityJilin Province Key Laboratory of Animal Nutrition and Feed Science, College of Animal Science and Technology, Key Lab of Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural UniversityJilin Province Key Laboratory of Animal Nutrition and Feed Science, College of Animal Science and Technology, Key Lab of Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural UniversityJilin Province Key Laboratory of Animal Nutrition and Feed Science, College of Animal Science and Technology, Key Lab of Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural UniversityDongfeng County Sika Deer Industry Development Service CenterJilin Province Key Laboratory of Animal Nutrition and Feed Science, College of Animal Science and Technology, Key Lab of Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural UniversityJilin Province Key Laboratory of Animal Nutrition and Feed Science, College of Animal Science and Technology, Key Lab of Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural UniversityJilin Province Key Laboratory of Animal Nutrition and Feed Science, College of Animal Science and Technology, Key Lab of Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural UniversityAbstract Background Promoting the synchronization of glucose and amino acid release in the digestive tract of pigs could effectively improve dietary nitrogen utilization. The rational allocation of dietary starch sources and the exploration of appropriate dietary glucose release kinetics may promote the dynamic balance of dietary glucose and amino acid supplies. However, research on the effects of diets with different glucose release kinetic profiles on amino acid absorption and portal amino acid appearance in piglets is limited. This study aimed to investigate the effects of the kinetic pattern of dietary glucose release on nitrogen utilization, the portal amino acid profile, and nutrient transporter expression in intestinal enterocytes in piglets. Methods Sixty-four barrows (15.00 ± 1.12 kg) were randomly allotted to 4 groups and fed diets formulated with starch from corn, corn/barley, corn/sorghum, or corn/cassava combinations (diets were coded A, B, C, or D respectively). Protein retention, the concentrations of portal amino acid and glucose, and the relative expression of amino acid and glucose transporter mRNAs were investigated. In vitro digestion was used to compare the dietary glucose release profiles. Results Four piglet diets with different glucose release kinetics were constructed by adjusting starch sources. The in vivo appearance dynamics of portal glucose were consistent with those of in vitro dietary glucose release kinetics. Total nitrogen excretion was reduced in the piglets in group B, while apparent nitrogen digestibility and nitrogen retention increased (P < 0.05). Regardless of the time (2 h or 4 h after morning feeding), the portal total free amino acids content and contents of some individual amino acids (Thr, Glu, Gly, Ala, and Ile) of the piglets in group B were significantly higher than those in groups A, C, and D (P < 0.05). Cluster analysis showed that different glucose release kinetic patterns resulted in different portal amino acid patterns in piglets, which decreased gradually with the extension of feeding time. The portal His/Phe, Pro/Glu, Leu/Val, Lys/Met, Tyr/Ile and Ala/Gly appeared higher similarity among the diet treatments. In the anterior jejunum, the glucose transporter SGLT1 was significantly positively correlated with the amino acid transporters B 0 AT1, EAAC1, and CAT1. Conclusions Rational allocation of starch resources could regulate dietary glucose release kinetics. In the present study, group B (corn/barley) diet exhibited a better glucose release kinetic pattern than the other groups, which could affect the portal amino acid contents and patterns by regulating the expression of amino acid transporters in the small intestine, thereby promoting nitrogen deposition in the body, and improving the utilization efficiency of dietary nitrogen.https://doi.org/10.1186/s40104-024-01000-zGlucose release kineticsNitrogen utilizationNutrient transporterPiglet |
| spellingShingle | Zexi Li Yunfei Li Yufei Zhao Guifu Wang Rujie Liu Yue Li Qamar Aftab Zewei Sun Qingzhen Zhong Effects of the kinetic pattern of dietary glucose release on nitrogen utilization, the portal amino acid profile, and nutrient transporter expression in intestinal enterocytes in piglets Journal of Animal Science and Biotechnology Glucose release kinetics Nitrogen utilization Nutrient transporter Piglet |
| title | Effects of the kinetic pattern of dietary glucose release on nitrogen utilization, the portal amino acid profile, and nutrient transporter expression in intestinal enterocytes in piglets |
| title_full | Effects of the kinetic pattern of dietary glucose release on nitrogen utilization, the portal amino acid profile, and nutrient transporter expression in intestinal enterocytes in piglets |
| title_fullStr | Effects of the kinetic pattern of dietary glucose release on nitrogen utilization, the portal amino acid profile, and nutrient transporter expression in intestinal enterocytes in piglets |
| title_full_unstemmed | Effects of the kinetic pattern of dietary glucose release on nitrogen utilization, the portal amino acid profile, and nutrient transporter expression in intestinal enterocytes in piglets |
| title_short | Effects of the kinetic pattern of dietary glucose release on nitrogen utilization, the portal amino acid profile, and nutrient transporter expression in intestinal enterocytes in piglets |
| title_sort | effects of the kinetic pattern of dietary glucose release on nitrogen utilization the portal amino acid profile and nutrient transporter expression in intestinal enterocytes in piglets |
| topic | Glucose release kinetics Nitrogen utilization Nutrient transporter Piglet |
| url | https://doi.org/10.1186/s40104-024-01000-z |
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