Interspaced Repeat Sequences Confer the Regulatory Functions of AtXTH10, Important for Root Growth in Arabidopsis
An interspaced repeat sequence (IRS) is a unique sequence similar to prokaryotic CRISPR in structure. In this study, 1343 IRSs were identified in the Arabidopsis genome. Functional annotation of the IRS-related genes showed that they were associated with various growth and development processes. Mor...
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| Format: | Article |
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MDPI AG
2019-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/8/5/130 |
| _version_ | 1819002757748621312 |
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| author | Jun Cao Yueqing Lv Xiang Li |
| author_facet | Jun Cao Yueqing Lv Xiang Li |
| author_sort | Jun Cao |
| collection | DOAJ |
| description | An interspaced repeat sequence (IRS) is a unique sequence similar to prokaryotic CRISPR in structure. In this study, 1343 IRSs were identified in the Arabidopsis genome. Functional annotation of the IRS-related genes showed that they were associated with various growth and development processes. More than 30% of the IRSs were located in promoter regions. Deletion of some IRSs affected promoter activity, suggesting their roles in the regulation of gene expression. Next, the function of the <i>AtXTH10</i> gene was further analyzed, and the expression of this gene was regulated by IRSs in its promoter region. Transgenic and mutant plants analysis indicated that the <i>AtXTH10</i> gene was associated with root development by affecting cell wall structure. Moreover, the expression profiles of some key genes involved in root development signaling pathways were also affected by <i>AtXTH10</i>. These results suggest that IRSs could be involved in regulating the expression of genes with important roles in plant development. |
| first_indexed | 2024-12-20T23:10:11Z |
| format | Article |
| id | doaj.art-7e5247ea9c164be88ca204232a1a7417 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-12-20T23:10:11Z |
| publishDate | 2019-05-01 |
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| series | Plants |
| spelling | doaj.art-7e5247ea9c164be88ca204232a1a74172022-12-21T19:23:45ZengMDPI AGPlants2223-77472019-05-018513010.3390/plants8050130plants8050130Interspaced Repeat Sequences Confer the Regulatory Functions of AtXTH10, Important for Root Growth in ArabidopsisJun Cao0Yueqing Lv1Xiang Li2Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, ChinaInstitute of Life Sciences, Jiangsu University, Zhenjiang 212013, ChinaInstitute of Life Sciences, Jiangsu University, Zhenjiang 212013, ChinaAn interspaced repeat sequence (IRS) is a unique sequence similar to prokaryotic CRISPR in structure. In this study, 1343 IRSs were identified in the Arabidopsis genome. Functional annotation of the IRS-related genes showed that they were associated with various growth and development processes. More than 30% of the IRSs were located in promoter regions. Deletion of some IRSs affected promoter activity, suggesting their roles in the regulation of gene expression. Next, the function of the <i>AtXTH10</i> gene was further analyzed, and the expression of this gene was regulated by IRSs in its promoter region. Transgenic and mutant plants analysis indicated that the <i>AtXTH10</i> gene was associated with root development by affecting cell wall structure. Moreover, the expression profiles of some key genes involved in root development signaling pathways were also affected by <i>AtXTH10</i>. These results suggest that IRSs could be involved in regulating the expression of genes with important roles in plant development.https://www.mdpi.com/2223-7747/8/5/130IRSspromotergene expression regulationxyloglucan endotransglucosylase/hydrolaseroot development |
| spellingShingle | Jun Cao Yueqing Lv Xiang Li Interspaced Repeat Sequences Confer the Regulatory Functions of AtXTH10, Important for Root Growth in Arabidopsis Plants IRSs promoter gene expression regulation xyloglucan endotransglucosylase/hydrolase root development |
| title | Interspaced Repeat Sequences Confer the Regulatory Functions of AtXTH10, Important for Root Growth in Arabidopsis |
| title_full | Interspaced Repeat Sequences Confer the Regulatory Functions of AtXTH10, Important for Root Growth in Arabidopsis |
| title_fullStr | Interspaced Repeat Sequences Confer the Regulatory Functions of AtXTH10, Important for Root Growth in Arabidopsis |
| title_full_unstemmed | Interspaced Repeat Sequences Confer the Regulatory Functions of AtXTH10, Important for Root Growth in Arabidopsis |
| title_short | Interspaced Repeat Sequences Confer the Regulatory Functions of AtXTH10, Important for Root Growth in Arabidopsis |
| title_sort | interspaced repeat sequences confer the regulatory functions of atxth10 important for root growth in arabidopsis |
| topic | IRSs promoter gene expression regulation xyloglucan endotransglucosylase/hydrolase root development |
| url | https://www.mdpi.com/2223-7747/8/5/130 |
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