Transcriptomic Analysis of Soil-Grown Arabidopsis thaliana Roots and Shoots in Response to a Drought Stress
Drought stress has a negative impact on crop yield. Thus, understanding the molecular mechanisms responsible for plant drought stress tolerance is essential for improving this beneficial trait in crops. In the current study, a transcriptional analysis was conducted of gene regulatory networks in roo...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2016-02-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00180/full |
| _version_ | 1818349341207691264 |
|---|---|
| author | Sultana eRasheed Sultana eRasheed Khurram eBashir Akihiro eMatsui Maho eTanaka Motoaki eSeki Motoaki eSeki Motoaki eSeki |
| author_facet | Sultana eRasheed Sultana eRasheed Khurram eBashir Akihiro eMatsui Maho eTanaka Motoaki eSeki Motoaki eSeki Motoaki eSeki |
| author_sort | Sultana eRasheed |
| collection | DOAJ |
| description | Drought stress has a negative impact on crop yield. Thus, understanding the molecular mechanisms responsible for plant drought stress tolerance is essential for improving this beneficial trait in crops. In the current study, a transcriptional analysis was conducted of gene regulatory networks in roots of soil-grown Arabidopsis plants in response to a drought stress treatment. A microarray analysis of drought-stressed roots and shoots was performed at 0, 1, 3, 5, 7 and 9 days. Results indicated that the expression of many drought stress-responsive genes and abscisic acid biosynthesis-related genes was differentially regulated in roots and shoots from days 3 to 9. The expression of cellular and metabolic process-related genes was up-regulated at an earlier time-point in roots than in shoots. In this regard, the expression of genes involved in oxidative signaling, chromatin structure, and cell wall modification also increased significantly in roots compared to shoots. Moreover, the increased expression of genes involved in the transport of amino acids and other solutes; including malate, iron, and sulfur, was observed in roots during the early time points following the initiation of the drought stress. These data suggest that plants may utilize these signaling channels and metabolic adjustments as adaptive responses in the early stages of a drought stress. Collectively, the results of the present study increases our understanding of the differences pertaining to the molecular mechanisms occurring in roots versus shoots in response to a drought stress. Furthermore, these findings also aid in the selection of novel genes and promoters that can be used to potentially produce crop plants with increased drought tolerance. |
| first_indexed | 2024-12-13T18:04:24Z |
| format | Article |
| id | doaj.art-7e53e9b2fdd44ef9ad076a3ec0d7a4af |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-13T18:04:24Z |
| publishDate | 2016-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-7e53e9b2fdd44ef9ad076a3ec0d7a4af2022-12-21T23:36:07ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-02-01710.3389/fpls.2016.00180183475Transcriptomic Analysis of Soil-Grown Arabidopsis thaliana Roots and Shoots in Response to a Drought StressSultana eRasheed0Sultana eRasheed1Khurram eBashir2Akihiro eMatsui3Maho eTanaka4Motoaki eSeki5Motoaki eSeki6Motoaki eSeki7RIKEN Center for Sustainable Resource ScienceYokohama City UniversityRIKEN Center for Sustainable Resource ScienceRIKEN Center for Sustainable Resource ScienceRIKEN Center for Sustainable Resource ScienceRIKEN Center for Sustainable Resource ScienceYokohama City UniversityCRESTDrought stress has a negative impact on crop yield. Thus, understanding the molecular mechanisms responsible for plant drought stress tolerance is essential for improving this beneficial trait in crops. In the current study, a transcriptional analysis was conducted of gene regulatory networks in roots of soil-grown Arabidopsis plants in response to a drought stress treatment. A microarray analysis of drought-stressed roots and shoots was performed at 0, 1, 3, 5, 7 and 9 days. Results indicated that the expression of many drought stress-responsive genes and abscisic acid biosynthesis-related genes was differentially regulated in roots and shoots from days 3 to 9. The expression of cellular and metabolic process-related genes was up-regulated at an earlier time-point in roots than in shoots. In this regard, the expression of genes involved in oxidative signaling, chromatin structure, and cell wall modification also increased significantly in roots compared to shoots. Moreover, the increased expression of genes involved in the transport of amino acids and other solutes; including malate, iron, and sulfur, was observed in roots during the early time points following the initiation of the drought stress. These data suggest that plants may utilize these signaling channels and metabolic adjustments as adaptive responses in the early stages of a drought stress. Collectively, the results of the present study increases our understanding of the differences pertaining to the molecular mechanisms occurring in roots versus shoots in response to a drought stress. Furthermore, these findings also aid in the selection of novel genes and promoters that can be used to potentially produce crop plants with increased drought tolerance.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00180/fullAbscisic AcidTranscription FactorsMicroarraydroughtArabidopsis thalianaabiotic stresses |
| spellingShingle | Sultana eRasheed Sultana eRasheed Khurram eBashir Akihiro eMatsui Maho eTanaka Motoaki eSeki Motoaki eSeki Motoaki eSeki Transcriptomic Analysis of Soil-Grown Arabidopsis thaliana Roots and Shoots in Response to a Drought Stress Frontiers in Plant Science Abscisic Acid Transcription Factors Microarray drought Arabidopsis thaliana abiotic stresses |
| title | Transcriptomic Analysis of Soil-Grown Arabidopsis thaliana Roots and Shoots in Response to a Drought Stress |
| title_full | Transcriptomic Analysis of Soil-Grown Arabidopsis thaliana Roots and Shoots in Response to a Drought Stress |
| title_fullStr | Transcriptomic Analysis of Soil-Grown Arabidopsis thaliana Roots and Shoots in Response to a Drought Stress |
| title_full_unstemmed | Transcriptomic Analysis of Soil-Grown Arabidopsis thaliana Roots and Shoots in Response to a Drought Stress |
| title_short | Transcriptomic Analysis of Soil-Grown Arabidopsis thaliana Roots and Shoots in Response to a Drought Stress |
| title_sort | transcriptomic analysis of soil grown arabidopsis thaliana roots and shoots in response to a drought stress |
| topic | Abscisic Acid Transcription Factors Microarray drought Arabidopsis thaliana abiotic stresses |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00180/full |
| work_keys_str_mv | AT sultanaerasheed transcriptomicanalysisofsoilgrownarabidopsisthalianarootsandshootsinresponsetoadroughtstress AT sultanaerasheed transcriptomicanalysisofsoilgrownarabidopsisthalianarootsandshootsinresponsetoadroughtstress AT khurramebashir transcriptomicanalysisofsoilgrownarabidopsisthalianarootsandshootsinresponsetoadroughtstress AT akihiroematsui transcriptomicanalysisofsoilgrownarabidopsisthalianarootsandshootsinresponsetoadroughtstress AT mahoetanaka transcriptomicanalysisofsoilgrownarabidopsisthalianarootsandshootsinresponsetoadroughtstress AT motoakieseki transcriptomicanalysisofsoilgrownarabidopsisthalianarootsandshootsinresponsetoadroughtstress AT motoakieseki transcriptomicanalysisofsoilgrownarabidopsisthalianarootsandshootsinresponsetoadroughtstress AT motoakieseki transcriptomicanalysisofsoilgrownarabidopsisthalianarootsandshootsinresponsetoadroughtstress |