A Transcriptomic Analysis of Gonads from the Low-Temperature-Induced Masculinization of <i>Takifugu rubripes</i>
The phenotypic sex of fish is usually plastic. Low-temperature treatment induces the masculinization of <i>Takifugu rubripes</i>, resulting in pseudo-males (PM) with the physiological sex of a male (M) and genetic sex of a female (F). For a comparison of gonadal transcriptomes, we collec...
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MDPI AG
2021-11-01
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| Online Access: | https://www.mdpi.com/2076-2615/11/12/3419 |
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| author | He Zhou Yuqing Sun Xin Li Ziyu Zhou Kexin Ma Wenxuan Guo Yuting Liang Xingyi Xie Jingxian Zhang Qian Wang Yang Liu |
| author_facet | He Zhou Yuqing Sun Xin Li Ziyu Zhou Kexin Ma Wenxuan Guo Yuting Liang Xingyi Xie Jingxian Zhang Qian Wang Yang Liu |
| author_sort | He Zhou |
| collection | DOAJ |
| description | The phenotypic sex of fish is usually plastic. Low-temperature treatment induces the masculinization of <i>Takifugu rubripes</i>, resulting in pseudo-males (PM) with the physiological sex of a male (M) and genetic sex of a female (F). For a comparison of gonadal transcriptomes, we collected gonads from three groups of <i>T. rubripes</i> (F, M, and PM) for high-throughput transcriptome sequencing. The results provided 467,640,218 raw reads (70.15 Gb) and a total of 436,151,088 clean reads (65.43 Gb), with an average length of 150 bp. Only 79 differentially expressed genes (DEGs) were identified between F and PM, whereas 12,041 and 11,528 DEGs were identified between F and M, and PM and M, respectively. According to the functional annotation of DEGs, 13 DEGs related to gonadal development were screened (<i>LOC101066759</i>, <i>dgat1</i>, <i>limk1</i>, <i>fbxl3</i>, <i>col6a3</i>, <i>fgfr3</i>, <i>dusp22b</i>, <i>svil</i>, <i>abhd17b</i>, <i>srgap3</i>, <i>tmem88b</i>, <i>bud4</i>, and <i>mustn10</i>) which might participate in formating PM. A quantitative PCR of the DEGs confirmed the reliability of the RNA-seq. Our results provide an important contribution to the genome sequence resources for <i>T. rubripes</i> and insight into the molecular mechanism of masculinization in a cultured fish subject to low-temperature treatment. |
| first_indexed | 2024-03-10T04:39:41Z |
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| language | English |
| last_indexed | 2024-03-10T04:39:41Z |
| publishDate | 2021-11-01 |
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| series | Animals |
| spelling | doaj.art-7e57e411acf74cb68a2b478293e56f922023-11-23T03:26:39ZengMDPI AGAnimals2076-26152021-11-011112341910.3390/ani11123419A Transcriptomic Analysis of Gonads from the Low-Temperature-Induced Masculinization of <i>Takifugu rubripes</i>He Zhou0Yuqing Sun1Xin Li2Ziyu Zhou3Kexin Ma4Wenxuan Guo5Yuting Liang6Xingyi Xie7Jingxian Zhang8Qian Wang9Yang Liu10Key Laboratory of Mariculture, Agriculture Ministry, PRC, Dalian Ocean University, Dalian 116023, ChinaKey Laboratory of Mariculture, Agriculture Ministry, PRC, Dalian Ocean University, Dalian 116023, ChinaKey Laboratory of Mariculture, Agriculture Ministry, PRC, Dalian Ocean University, Dalian 116023, ChinaKey Laboratory of Mariculture, Agriculture Ministry, PRC, Dalian Ocean University, Dalian 116023, ChinaKey Laboratory of Mariculture, Agriculture Ministry, PRC, Dalian Ocean University, Dalian 116023, ChinaKey Laboratory of Mariculture, Agriculture Ministry, PRC, Dalian Ocean University, Dalian 116023, ChinaKey Laboratory of Mariculture, Agriculture Ministry, PRC, Dalian Ocean University, Dalian 116023, ChinaKey Laboratory of Mariculture, Agriculture Ministry, PRC, Dalian Ocean University, Dalian 116023, ChinaKey Laboratory of Mariculture, Agriculture Ministry, PRC, Dalian Ocean University, Dalian 116023, ChinaKey Lab of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaKey Laboratory of Mariculture, Agriculture Ministry, PRC, Dalian Ocean University, Dalian 116023, ChinaThe phenotypic sex of fish is usually plastic. Low-temperature treatment induces the masculinization of <i>Takifugu rubripes</i>, resulting in pseudo-males (PM) with the physiological sex of a male (M) and genetic sex of a female (F). For a comparison of gonadal transcriptomes, we collected gonads from three groups of <i>T. rubripes</i> (F, M, and PM) for high-throughput transcriptome sequencing. The results provided 467,640,218 raw reads (70.15 Gb) and a total of 436,151,088 clean reads (65.43 Gb), with an average length of 150 bp. Only 79 differentially expressed genes (DEGs) were identified between F and PM, whereas 12,041 and 11,528 DEGs were identified between F and M, and PM and M, respectively. According to the functional annotation of DEGs, 13 DEGs related to gonadal development were screened (<i>LOC101066759</i>, <i>dgat1</i>, <i>limk1</i>, <i>fbxl3</i>, <i>col6a3</i>, <i>fgfr3</i>, <i>dusp22b</i>, <i>svil</i>, <i>abhd17b</i>, <i>srgap3</i>, <i>tmem88b</i>, <i>bud4</i>, and <i>mustn10</i>) which might participate in formating PM. A quantitative PCR of the DEGs confirmed the reliability of the RNA-seq. Our results provide an important contribution to the genome sequence resources for <i>T. rubripes</i> and insight into the molecular mechanism of masculinization in a cultured fish subject to low-temperature treatment.https://www.mdpi.com/2076-2615/11/12/3419transcriptomiclow-temperaturepseudo-maleqPCR<i>Takifugu rubripes</i> |
| spellingShingle | He Zhou Yuqing Sun Xin Li Ziyu Zhou Kexin Ma Wenxuan Guo Yuting Liang Xingyi Xie Jingxian Zhang Qian Wang Yang Liu A Transcriptomic Analysis of Gonads from the Low-Temperature-Induced Masculinization of <i>Takifugu rubripes</i> Animals transcriptomic low-temperature pseudo-male qPCR <i>Takifugu rubripes</i> |
| title | A Transcriptomic Analysis of Gonads from the Low-Temperature-Induced Masculinization of <i>Takifugu rubripes</i> |
| title_full | A Transcriptomic Analysis of Gonads from the Low-Temperature-Induced Masculinization of <i>Takifugu rubripes</i> |
| title_fullStr | A Transcriptomic Analysis of Gonads from the Low-Temperature-Induced Masculinization of <i>Takifugu rubripes</i> |
| title_full_unstemmed | A Transcriptomic Analysis of Gonads from the Low-Temperature-Induced Masculinization of <i>Takifugu rubripes</i> |
| title_short | A Transcriptomic Analysis of Gonads from the Low-Temperature-Induced Masculinization of <i>Takifugu rubripes</i> |
| title_sort | transcriptomic analysis of gonads from the low temperature induced masculinization of i takifugu rubripes i |
| topic | transcriptomic low-temperature pseudo-male qPCR <i>Takifugu rubripes</i> |
| url | https://www.mdpi.com/2076-2615/11/12/3419 |
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