Construction and use of <it>Plasmodium falciparum </it>phage display libraries to identify host parasite interactions
<p>Abstract</p> <p>Background</p> <p>The development of <it>Plasmodium falciparum </it>within human erythrocytes induces a wide array of changes in the ultrastructure, function and antigenic properties of the host cell. Numerous proteins encoded by the paras...
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| Format: | Article |
| Language: | English |
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BMC
2003-12-01
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| Series: | Malaria Journal |
| Online Access: | http://www.malariajournal.com/content/2/1/47 |
| _version_ | 1818756512420462592 |
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| author | Coetzer Theresa L Lanzillotti Roberto Lauterbach Sonja B |
| author_facet | Coetzer Theresa L Lanzillotti Roberto Lauterbach Sonja B |
| author_sort | Coetzer Theresa L |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The development of <it>Plasmodium falciparum </it>within human erythrocytes induces a wide array of changes in the ultrastructure, function and antigenic properties of the host cell. Numerous proteins encoded by the parasite have been shown to interact with the erythrocyte membrane. The identification of new interactions between human erythrocyte and <it>P. falciparum </it>proteins has formed a key area of malaria research. To circumvent the difficulties provided by conventional protein techniques, a novel application of the phage display technology was utilised.</p> <p>Methods</p> <p><it>P. falciparum </it>phage display libraries were created and biopanned against purified erythrocyte membrane proteins. The identification of interacting and in-frame amino acid sequences was achieved by sequencing parasite cDNA inserts and performing bioinformatic analyses in the PlasmoDB database.</p> <p>Results</p> <p>Following four rounds of biopanning, sequencing and bioinformatic investigations, seven <it>P. falciparum </it>proteins with significant binding specificity toward human erythrocyte spectrin and protein 4.1 were identified. The specificity of these <it>P. falciparum </it>proteins were demonstrated by the marked enrichment of the respective in-frame binding sequences from a fourth round phage display library.</p> <p>Conclusion</p> <p>The construction and biopanning of <it>P. falciparum </it>phage display expression libraries provide a novel approach for the identification of new interactions between the parasite and the erythrocyte membrane.</p> |
| first_indexed | 2024-12-18T05:56:13Z |
| format | Article |
| id | doaj.art-7e58b98249104b20a813e2fab6f01f68 |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-12-18T05:56:13Z |
| publishDate | 2003-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-7e58b98249104b20a813e2fab6f01f682022-12-21T21:18:47ZengBMCMalaria Journal1475-28752003-12-01214710.1186/1475-2875-2-47Construction and use of <it>Plasmodium falciparum </it>phage display libraries to identify host parasite interactionsCoetzer Theresa LLanzillotti RobertoLauterbach Sonja B<p>Abstract</p> <p>Background</p> <p>The development of <it>Plasmodium falciparum </it>within human erythrocytes induces a wide array of changes in the ultrastructure, function and antigenic properties of the host cell. Numerous proteins encoded by the parasite have been shown to interact with the erythrocyte membrane. The identification of new interactions between human erythrocyte and <it>P. falciparum </it>proteins has formed a key area of malaria research. To circumvent the difficulties provided by conventional protein techniques, a novel application of the phage display technology was utilised.</p> <p>Methods</p> <p><it>P. falciparum </it>phage display libraries were created and biopanned against purified erythrocyte membrane proteins. The identification of interacting and in-frame amino acid sequences was achieved by sequencing parasite cDNA inserts and performing bioinformatic analyses in the PlasmoDB database.</p> <p>Results</p> <p>Following four rounds of biopanning, sequencing and bioinformatic investigations, seven <it>P. falciparum </it>proteins with significant binding specificity toward human erythrocyte spectrin and protein 4.1 were identified. The specificity of these <it>P. falciparum </it>proteins were demonstrated by the marked enrichment of the respective in-frame binding sequences from a fourth round phage display library.</p> <p>Conclusion</p> <p>The construction and biopanning of <it>P. falciparum </it>phage display expression libraries provide a novel approach for the identification of new interactions between the parasite and the erythrocyte membrane.</p>http://www.malariajournal.com/content/2/1/47 |
| spellingShingle | Coetzer Theresa L Lanzillotti Roberto Lauterbach Sonja B Construction and use of <it>Plasmodium falciparum </it>phage display libraries to identify host parasite interactions Malaria Journal |
| title | Construction and use of <it>Plasmodium falciparum </it>phage display libraries to identify host parasite interactions |
| title_full | Construction and use of <it>Plasmodium falciparum </it>phage display libraries to identify host parasite interactions |
| title_fullStr | Construction and use of <it>Plasmodium falciparum </it>phage display libraries to identify host parasite interactions |
| title_full_unstemmed | Construction and use of <it>Plasmodium falciparum </it>phage display libraries to identify host parasite interactions |
| title_short | Construction and use of <it>Plasmodium falciparum </it>phage display libraries to identify host parasite interactions |
| title_sort | construction and use of it plasmodium falciparum it phage display libraries to identify host parasite interactions |
| url | http://www.malariajournal.com/content/2/1/47 |
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