<em>Thiorhodovibrio frisius</em> and <em>Trv. litoralis</em> spp. nov., Two Novel Members from a Clade of Fastidious Purple Sulfur Bacteria That Exhibit Unique Red-Shifted Light-Harvesting Capabilities
In the pursuit of cultivating anaerobic anoxygenic phototrophs with unusual absorbance spectra, a purple sulfur bacterium was isolated from the shoreline of Baltrum, a North Sea island of Germany. It was designated strain 970, due to a predominant light harvesting complex (LH) absorption maximum at...
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MDPI AG
2023-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/10/2394 |
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| author | Anika Methner Steven B. Kuzyk Jörn Petersen Sabine Bauer Henner Brinkmann Katja Sichau Gerhard Wanner Jacqueline Wolf Meina Neumann-Schaal Petra Henke Marcus Tank Cathrin Spröer Boyke Bunk Jörg Overmann |
| author_facet | Anika Methner Steven B. Kuzyk Jörn Petersen Sabine Bauer Henner Brinkmann Katja Sichau Gerhard Wanner Jacqueline Wolf Meina Neumann-Schaal Petra Henke Marcus Tank Cathrin Spröer Boyke Bunk Jörg Overmann |
| author_sort | Anika Methner |
| collection | DOAJ |
| description | In the pursuit of cultivating anaerobic anoxygenic phototrophs with unusual absorbance spectra, a purple sulfur bacterium was isolated from the shoreline of Baltrum, a North Sea island of Germany. It was designated strain 970, due to a predominant light harvesting complex (LH) absorption maximum at 963–966 nm, which represents the furthest infrared-shift documented for such complexes containing bacteriochlorophyll <i>a</i>. A polyphasic approach to bacterial systematics was performed, comparing genomic, biochemical, and physiological properties. Strain 970 is related to <i>Thiorhodovibrio winogradskyi</i> DSM 6702<sup>T</sup> by 26.5, 81.9, and 98.0% similarity via dDDH, ANI, and 16S rRNA gene comparisons, respectively. The photosynthetic properties of strain 970 were unlike other <i>Thiorhodovibrio</i> spp., which contained typical LH absorbing characteristics of 800–870 nm, as well as a newly discovered absorption band at 908 nm. Strain 970 also had a different photosynthetic operon composition. Upon genomic comparisons with the original <i>Thiorhodovibrio</i> strains DSM 6702<sup>T</sup> and strain 06511, the latter was found to be divergent, with 25.3, 79.1, and 97.5% similarity via dDDH, ANI, and 16S rRNA gene homology to <i>Trv. winogradskyi</i>, respectively. Strain 06511 (=DSM 116345<sup>T</sup>) is thereby described as <i>Thiorhodovibrio litoralis</i> sp. nov., and the unique strain 970 (=DSM 111777<sup>T</sup>) as <i>Thiorhodovibrio frisius</i> sp. nov. |
| first_indexed | 2024-03-10T21:02:39Z |
| format | Article |
| id | doaj.art-7e58da2d2f2b4b60860a01f5fc6fa8de |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T21:02:39Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-7e58da2d2f2b4b60860a01f5fc6fa8de2023-11-19T17:26:04ZengMDPI AGMicroorganisms2076-26072023-09-011110239410.3390/microorganisms11102394<em>Thiorhodovibrio frisius</em> and <em>Trv. litoralis</em> spp. nov., Two Novel Members from a Clade of Fastidious Purple Sulfur Bacteria That Exhibit Unique Red-Shifted Light-Harvesting CapabilitiesAnika Methner0Steven B. Kuzyk1Jörn Petersen2Sabine Bauer3Henner Brinkmann4Katja Sichau5Gerhard Wanner6Jacqueline Wolf7Meina Neumann-Schaal8Petra Henke9Marcus Tank10Cathrin Spröer11Boyke Bunk12Jörg Overmann13Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, Inhoffenstraße 7B, 38124 Braunschweig, GermanyLeibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, Inhoffenstraße 7B, 38124 Braunschweig, GermanyLeibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, Inhoffenstraße 7B, 38124 Braunschweig, GermanyFormer Institution: Paläomikrobiologie, Institut für Chemie und Biologie des Meeres, Universität Oldenburg, Postfach 2503, 26111 Oldenburg, GermanyLeibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, Inhoffenstraße 7B, 38124 Braunschweig, GermanyBereich Mikrobiologie, Department Biologie I, Ludwig-Maximilians-Universität München, Großhaderner Str. 2-4, 82152 Planegg-Martinsried, GermanyBereich Mikrobiologie, Department Biologie I, Ludwig-Maximilians-Universität München, Großhaderner Str. 2-4, 82152 Planegg-Martinsried, GermanyLeibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, Inhoffenstraße 7B, 38124 Braunschweig, GermanyLeibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, Inhoffenstraße 7B, 38124 Braunschweig, GermanyLeibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, Inhoffenstraße 7B, 38124 Braunschweig, GermanyLeibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, Inhoffenstraße 7B, 38124 Braunschweig, GermanyLeibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, Inhoffenstraße 7B, 38124 Braunschweig, GermanyLeibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, Inhoffenstraße 7B, 38124 Braunschweig, GermanyLeibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, Inhoffenstraße 7B, 38124 Braunschweig, GermanyIn the pursuit of cultivating anaerobic anoxygenic phototrophs with unusual absorbance spectra, a purple sulfur bacterium was isolated from the shoreline of Baltrum, a North Sea island of Germany. It was designated strain 970, due to a predominant light harvesting complex (LH) absorption maximum at 963–966 nm, which represents the furthest infrared-shift documented for such complexes containing bacteriochlorophyll <i>a</i>. A polyphasic approach to bacterial systematics was performed, comparing genomic, biochemical, and physiological properties. Strain 970 is related to <i>Thiorhodovibrio winogradskyi</i> DSM 6702<sup>T</sup> by 26.5, 81.9, and 98.0% similarity via dDDH, ANI, and 16S rRNA gene comparisons, respectively. The photosynthetic properties of strain 970 were unlike other <i>Thiorhodovibrio</i> spp., which contained typical LH absorbing characteristics of 800–870 nm, as well as a newly discovered absorption band at 908 nm. Strain 970 also had a different photosynthetic operon composition. Upon genomic comparisons with the original <i>Thiorhodovibrio</i> strains DSM 6702<sup>T</sup> and strain 06511, the latter was found to be divergent, with 25.3, 79.1, and 97.5% similarity via dDDH, ANI, and 16S rRNA gene homology to <i>Trv. winogradskyi</i>, respectively. Strain 06511 (=DSM 116345<sup>T</sup>) is thereby described as <i>Thiorhodovibrio litoralis</i> sp. nov., and the unique strain 970 (=DSM 111777<sup>T</sup>) as <i>Thiorhodovibrio frisius</i> sp. nov.https://www.mdpi.com/2076-2607/11/10/2394anaerobic anoxygenic photosynthesis<i>Chromatiaceae</i>infrared light absorptionmicrobial matsphotosynthetic gene clusterpurple sulfur bacteria |
| spellingShingle | Anika Methner Steven B. Kuzyk Jörn Petersen Sabine Bauer Henner Brinkmann Katja Sichau Gerhard Wanner Jacqueline Wolf Meina Neumann-Schaal Petra Henke Marcus Tank Cathrin Spröer Boyke Bunk Jörg Overmann <em>Thiorhodovibrio frisius</em> and <em>Trv. litoralis</em> spp. nov., Two Novel Members from a Clade of Fastidious Purple Sulfur Bacteria That Exhibit Unique Red-Shifted Light-Harvesting Capabilities Microorganisms anaerobic anoxygenic photosynthesis <i>Chromatiaceae</i> infrared light absorption microbial mats photosynthetic gene cluster purple sulfur bacteria |
| title | <em>Thiorhodovibrio frisius</em> and <em>Trv. litoralis</em> spp. nov., Two Novel Members from a Clade of Fastidious Purple Sulfur Bacteria That Exhibit Unique Red-Shifted Light-Harvesting Capabilities |
| title_full | <em>Thiorhodovibrio frisius</em> and <em>Trv. litoralis</em> spp. nov., Two Novel Members from a Clade of Fastidious Purple Sulfur Bacteria That Exhibit Unique Red-Shifted Light-Harvesting Capabilities |
| title_fullStr | <em>Thiorhodovibrio frisius</em> and <em>Trv. litoralis</em> spp. nov., Two Novel Members from a Clade of Fastidious Purple Sulfur Bacteria That Exhibit Unique Red-Shifted Light-Harvesting Capabilities |
| title_full_unstemmed | <em>Thiorhodovibrio frisius</em> and <em>Trv. litoralis</em> spp. nov., Two Novel Members from a Clade of Fastidious Purple Sulfur Bacteria That Exhibit Unique Red-Shifted Light-Harvesting Capabilities |
| title_short | <em>Thiorhodovibrio frisius</em> and <em>Trv. litoralis</em> spp. nov., Two Novel Members from a Clade of Fastidious Purple Sulfur Bacteria That Exhibit Unique Red-Shifted Light-Harvesting Capabilities |
| title_sort | em thiorhodovibrio frisius em and em trv litoralis em spp nov two novel members from a clade of fastidious purple sulfur bacteria that exhibit unique red shifted light harvesting capabilities |
| topic | anaerobic anoxygenic photosynthesis <i>Chromatiaceae</i> infrared light absorption microbial mats photosynthetic gene cluster purple sulfur bacteria |
| url | https://www.mdpi.com/2076-2607/11/10/2394 |
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