Investigation of the Effect of Pumpkin (Cucurbita pepo L.) Seed Oil on Pentylenetetrazole-induced Neuronal Damage in HT-22 Cell Line
Recent studies have shown the positive effects of Cucurbita pepo L. (pumpkin) seed oil (PSO) in different disease models. However, the effect of PSO on neurological diseases has not been clarified yet. Therefore, this study aims to elucidate the effects of BBS on pentylenetetrazole (PTZ)-induced neu...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Turkish Science and Technology Publishing (TURSTEP)
2023-12-01
|
| Series: | Turkish Journal of Agriculture: Food Science and Technology |
| Subjects: | |
| Online Access: | https://www.agrifoodscience.com/index.php/TURJAF/article/view/6410 |
| _version_ | 1797364653538410496 |
|---|---|
| author | Ahmet Taskiran Tuğba Yıldız Asdemir |
| author_facet | Ahmet Taskiran Tuğba Yıldız Asdemir |
| author_sort | Ahmet Taskiran |
| collection | DOAJ |
| description | Recent studies have shown the positive effects of Cucurbita pepo L. (pumpkin) seed oil (PSO) in different disease models. However, the effect of PSO on neurological diseases has not been clarified yet. Therefore, this study aims to elucidate the effects of BBS on pentylenetetrazole (PTZ)-induced neuronal damage and the possible roles of oxidative and nitrosative stress in this effect in vitro. The HT-22 hippocampal neuronal cell line was used in the study. Cell survival after PTZ-induced neuronal damage was evaluated with the XTT test in the groups. While the effects of BBS on total antioxidant status (TAS) and total oxidant status (TOS) after PTZ were measured with colorimetric commercial kits, its effects on neuronal nitric oxide synthase (nNOS) and nitric oxide (NO) levels were also determined by ELISA kits. In light of the data obtained, it was found that pre-treatment with PSO prevented the decrease in cell survival after exposure to PTZ. In addition, it has been found that PSO normalizes the increase in TOS, nNOS, and NO in neuronal cells after PTZ. As a result, it was determined that the treatment of neuronal cells with PSO prevented neuronal damage caused by PTZ and showed neuroprotective properties. It is thought that PSO may achieve these effects through oxidative and nitrosative systems. Enrichment of a daily diet with PSO might be beneficial in reducing the risks of neurological diseases. |
| first_indexed | 2024-03-08T16:38:20Z |
| format | Article |
| id | doaj.art-7e5afc029cea452ea4f590161d587f89 |
| institution | Directory Open Access Journal |
| issn | 2148-127X |
| language | English |
| last_indexed | 2024-03-08T16:38:20Z |
| publishDate | 2023-12-01 |
| publisher | Turkish Science and Technology Publishing (TURSTEP) |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj.art-7e5afc029cea452ea4f590161d587f892024-01-05T12:06:43ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2023-12-0111s12499250410.24925/turjaf.v11is1.2499-2504.64105111Investigation of the Effect of Pumpkin (Cucurbita pepo L.) Seed Oil on Pentylenetetrazole-induced Neuronal Damage in HT-22 Cell LineAhmet Taskiran0https://orcid.org/0000-0002-5810-8415Tuğba Yıldız Asdemir1https://orcid.org/0000-0002-5810-8415Departments of Physiology, School of Medicine, Cumhuriyet University, Sivas, TurkeyDepartments of Physiology, School of Medicine, Cumhuriyet University, Sivas, TurkeyRecent studies have shown the positive effects of Cucurbita pepo L. (pumpkin) seed oil (PSO) in different disease models. However, the effect of PSO on neurological diseases has not been clarified yet. Therefore, this study aims to elucidate the effects of BBS on pentylenetetrazole (PTZ)-induced neuronal damage and the possible roles of oxidative and nitrosative stress in this effect in vitro. The HT-22 hippocampal neuronal cell line was used in the study. Cell survival after PTZ-induced neuronal damage was evaluated with the XTT test in the groups. While the effects of BBS on total antioxidant status (TAS) and total oxidant status (TOS) after PTZ were measured with colorimetric commercial kits, its effects on neuronal nitric oxide synthase (nNOS) and nitric oxide (NO) levels were also determined by ELISA kits. In light of the data obtained, it was found that pre-treatment with PSO prevented the decrease in cell survival after exposure to PTZ. In addition, it has been found that PSO normalizes the increase in TOS, nNOS, and NO in neuronal cells after PTZ. As a result, it was determined that the treatment of neuronal cells with PSO prevented neuronal damage caused by PTZ and showed neuroprotective properties. It is thought that PSO may achieve these effects through oxidative and nitrosative systems. Enrichment of a daily diet with PSO might be beneficial in reducing the risks of neurological diseases.https://www.agrifoodscience.com/index.php/TURJAF/article/view/6410pumpkin seed oilpentylenetetrazoleoxidative stressnitrosative stressht-22 cells |
| spellingShingle | Ahmet Taskiran Tuğba Yıldız Asdemir Investigation of the Effect of Pumpkin (Cucurbita pepo L.) Seed Oil on Pentylenetetrazole-induced Neuronal Damage in HT-22 Cell Line Turkish Journal of Agriculture: Food Science and Technology pumpkin seed oil pentylenetetrazole oxidative stress nitrosative stress ht-22 cells |
| title | Investigation of the Effect of Pumpkin (Cucurbita pepo L.) Seed Oil on Pentylenetetrazole-induced Neuronal Damage in HT-22 Cell Line |
| title_full | Investigation of the Effect of Pumpkin (Cucurbita pepo L.) Seed Oil on Pentylenetetrazole-induced Neuronal Damage in HT-22 Cell Line |
| title_fullStr | Investigation of the Effect of Pumpkin (Cucurbita pepo L.) Seed Oil on Pentylenetetrazole-induced Neuronal Damage in HT-22 Cell Line |
| title_full_unstemmed | Investigation of the Effect of Pumpkin (Cucurbita pepo L.) Seed Oil on Pentylenetetrazole-induced Neuronal Damage in HT-22 Cell Line |
| title_short | Investigation of the Effect of Pumpkin (Cucurbita pepo L.) Seed Oil on Pentylenetetrazole-induced Neuronal Damage in HT-22 Cell Line |
| title_sort | investigation of the effect of pumpkin cucurbita pepo l seed oil on pentylenetetrazole induced neuronal damage in ht 22 cell line |
| topic | pumpkin seed oil pentylenetetrazole oxidative stress nitrosative stress ht-22 cells |
| url | https://www.agrifoodscience.com/index.php/TURJAF/article/view/6410 |
| work_keys_str_mv | AT ahmettaskiran investigationoftheeffectofpumpkincucurbitapepolseedoilonpentylenetetrazoleinducedneuronaldamageinht22cellline AT tugbayıldızasdemir investigationoftheeffectofpumpkincucurbitapepolseedoilonpentylenetetrazoleinducedneuronaldamageinht22cellline |