Choosing source of microorganisms and processing technology for next generation beet bioinoculant
Abstract The increase of human population and associated increasing demand for agricultural products lead to soil over-exploitation. Biofertilizers based on lyophilized plant material containing living plant growth-promoting microorganisms (PGPM) could be an alternative to conventional fertilizers t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-82436-5 |
| _version_ | 1818692374982819840 |
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| author | Sonia Szymańska Marcin Sikora Katarzyna Hrynkiewicz Jarosław Tyburski Andrzej Tretyn Marcin Gołębiewski |
| author_facet | Sonia Szymańska Marcin Sikora Katarzyna Hrynkiewicz Jarosław Tyburski Andrzej Tretyn Marcin Gołębiewski |
| author_sort | Sonia Szymańska |
| collection | DOAJ |
| description | Abstract The increase of human population and associated increasing demand for agricultural products lead to soil over-exploitation. Biofertilizers based on lyophilized plant material containing living plant growth-promoting microorganisms (PGPM) could be an alternative to conventional fertilizers that fits into sustainable agricultural technologies ideas. We aimed to: (1) assess the diversity of endophytic bacteria in sugar and sea beet roots and (2) determine the influence of osmoprotectants (trehalose and ectoine) addition during lyophilization on bacterial density, viability and salt tolerance. Microbiome diversity was assessed based on 16S rRNA amplicons sequencing, bacterial density and salt tolerance was evaluated in cultures, while bacterial viability was calculated by using fluorescence microscopy and flow cytometry. Here we show that plant genotype shapes its endophytic microbiome diversity and determines rhizosphere soil properties. Sea beet endophytic microbiome, consisting of genera characteristic for extreme environments, is more diverse and salt resistant than its crop relative. Supplementing osmoprotectants during root tissue lyophilization exerts a positive effect on bacterial community salt stress tolerance, viability and density. Trehalose improves the above-mentioned parameters more effectively than ectoine, moreover its use is economically advantageous, thus it may be used to formulate improved biofertilizers. |
| first_indexed | 2024-12-17T12:56:47Z |
| format | Article |
| id | doaj.art-7e5b1fa628064308920c5350a298cc60 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-17T12:56:47Z |
| publishDate | 2021-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-7e5b1fa628064308920c5350a298cc602022-12-21T21:47:28ZengNature PortfolioScientific Reports2045-23222021-02-0111111310.1038/s41598-021-82436-5Choosing source of microorganisms and processing technology for next generation beet bioinoculantSonia Szymańska0Marcin Sikora1Katarzyna Hrynkiewicz2Jarosław Tyburski3Andrzej Tretyn4Marcin Gołębiewski5Department of Microbiology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University (NCU)Center for Modern Interdisciplinary Technologies, Nicolaus Copernicus University (NCU)Department of Microbiology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University (NCU)Center for Modern Interdisciplinary Technologies, Nicolaus Copernicus University (NCU)Center for Modern Interdisciplinary Technologies, Nicolaus Copernicus University (NCU)Center for Modern Interdisciplinary Technologies, Nicolaus Copernicus University (NCU)Abstract The increase of human population and associated increasing demand for agricultural products lead to soil over-exploitation. Biofertilizers based on lyophilized plant material containing living plant growth-promoting microorganisms (PGPM) could be an alternative to conventional fertilizers that fits into sustainable agricultural technologies ideas. We aimed to: (1) assess the diversity of endophytic bacteria in sugar and sea beet roots and (2) determine the influence of osmoprotectants (trehalose and ectoine) addition during lyophilization on bacterial density, viability and salt tolerance. Microbiome diversity was assessed based on 16S rRNA amplicons sequencing, bacterial density and salt tolerance was evaluated in cultures, while bacterial viability was calculated by using fluorescence microscopy and flow cytometry. Here we show that plant genotype shapes its endophytic microbiome diversity and determines rhizosphere soil properties. Sea beet endophytic microbiome, consisting of genera characteristic for extreme environments, is more diverse and salt resistant than its crop relative. Supplementing osmoprotectants during root tissue lyophilization exerts a positive effect on bacterial community salt stress tolerance, viability and density. Trehalose improves the above-mentioned parameters more effectively than ectoine, moreover its use is economically advantageous, thus it may be used to formulate improved biofertilizers.https://doi.org/10.1038/s41598-021-82436-5 |
| spellingShingle | Sonia Szymańska Marcin Sikora Katarzyna Hrynkiewicz Jarosław Tyburski Andrzej Tretyn Marcin Gołębiewski Choosing source of microorganisms and processing technology for next generation beet bioinoculant Scientific Reports |
| title | Choosing source of microorganisms and processing technology for next generation beet bioinoculant |
| title_full | Choosing source of microorganisms and processing technology for next generation beet bioinoculant |
| title_fullStr | Choosing source of microorganisms and processing technology for next generation beet bioinoculant |
| title_full_unstemmed | Choosing source of microorganisms and processing technology for next generation beet bioinoculant |
| title_short | Choosing source of microorganisms and processing technology for next generation beet bioinoculant |
| title_sort | choosing source of microorganisms and processing technology for next generation beet bioinoculant |
| url | https://doi.org/10.1038/s41598-021-82436-5 |
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