Fatty Acids from <i>Hermetia illucens</i> Larvae Fat Inhibit the Proliferation and Growth of Actual Phytopathogens
The rapid increase of plant diseases caused by bacterial phytopathogens calls for an urgent search for new antibacterials. Antimicrobial compounds of natural origin stand up as frontiers in the attempts of the antibiotic overuse replacement. With this in mind, the <i>Hermetia illucens (H. illu...
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MDPI AG
2020-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/9/1423 |
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| author | Elena Marusich Heakal Mohamed Yuriy Afanasev Sergey Leonov |
| author_facet | Elena Marusich Heakal Mohamed Yuriy Afanasev Sergey Leonov |
| author_sort | Elena Marusich |
| collection | DOAJ |
| description | The rapid increase of plant diseases caused by bacterial phytopathogens calls for an urgent search for new antibacterials. Antimicrobial compounds of natural origin stand up as frontiers in the attempts of the antibiotic overuse replacement. With this in mind, the <i>Hermetia illucens (H. illucens)</i> larvae have recently gained attention as a promising approach to fulfill this need. This study aimed to isolate the active constituents of <i>H. illucens</i> larvae fat and to estimate its antimicrobial capacity. We discovered the best composition of extracting solution retaining the pronounced antimicrobial activity of the extract. Using gas chromatography-mass spectrometry (GC-MS), we identified the unique natural array of fatty acids as the major constituents of the acidified water-methanol extract (AWME) as having new antimicrobial potency. In standard turbidimetric assay, the minimum inhibitory concentration (MIC) of the AWME was 0.78 mg/mL after 24 h of incubation for all five tested phytopathogenic bacteria strains: <i>Pantoea agglomerans, Xanthomonas campestris</i>, <i>Pectobacterium carotovorum subsp. carotovorum, Pectobacterium atrosepticum,</i> and <i>Dickeya solani</i>. The minimum bactericidal concentration (MBC) ranged from 0.78 to 1.56 mg/mL against all tested strains after 24 h of incubation. The inhibition zone size of AWME (INZ) at 50 mg/mL concentration was in the range 12.2 ± 0.56 to 19.0 ± 0.28 mm, while zone size for the positive control (penicillin-streptomycin) (5000 IU/mL–5000 µg/mL) was in the scale of 20.63 ± 0.53 to 24.0 ± 0.35 mm as revealed by standard disk diffusion assay. For the first time, our findings indicated the substantial antibacterial potential of AWME of <i>H. illucens</i> larvae fat against these actual phytopathogens, thus paving the way for further research to determine the mechanism of action in crop protection. |
| first_indexed | 2024-03-10T16:16:57Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T16:16:57Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-7e5b64af55184dd1a1ac3937a540c6972023-11-20T13:59:05ZengMDPI AGMicroorganisms2076-26072020-09-0189142310.3390/microorganisms8091423Fatty Acids from <i>Hermetia illucens</i> Larvae Fat Inhibit the Proliferation and Growth of Actual PhytopathogensElena Marusich0Heakal Mohamed1Yuriy Afanasev2Sergey Leonov3School of Biological and Medical Physics, Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Moscow Region, RussiaSchool of Biological and Medical Physics, Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Moscow Region, RussiaSchool of Biological and Medical Physics, Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Moscow Region, RussiaSchool of Biological and Medical Physics, Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Moscow Region, RussiaThe rapid increase of plant diseases caused by bacterial phytopathogens calls for an urgent search for new antibacterials. Antimicrobial compounds of natural origin stand up as frontiers in the attempts of the antibiotic overuse replacement. With this in mind, the <i>Hermetia illucens (H. illucens)</i> larvae have recently gained attention as a promising approach to fulfill this need. This study aimed to isolate the active constituents of <i>H. illucens</i> larvae fat and to estimate its antimicrobial capacity. We discovered the best composition of extracting solution retaining the pronounced antimicrobial activity of the extract. Using gas chromatography-mass spectrometry (GC-MS), we identified the unique natural array of fatty acids as the major constituents of the acidified water-methanol extract (AWME) as having new antimicrobial potency. In standard turbidimetric assay, the minimum inhibitory concentration (MIC) of the AWME was 0.78 mg/mL after 24 h of incubation for all five tested phytopathogenic bacteria strains: <i>Pantoea agglomerans, Xanthomonas campestris</i>, <i>Pectobacterium carotovorum subsp. carotovorum, Pectobacterium atrosepticum,</i> and <i>Dickeya solani</i>. The minimum bactericidal concentration (MBC) ranged from 0.78 to 1.56 mg/mL against all tested strains after 24 h of incubation. The inhibition zone size of AWME (INZ) at 50 mg/mL concentration was in the range 12.2 ± 0.56 to 19.0 ± 0.28 mm, while zone size for the positive control (penicillin-streptomycin) (5000 IU/mL–5000 µg/mL) was in the scale of 20.63 ± 0.53 to 24.0 ± 0.35 mm as revealed by standard disk diffusion assay. For the first time, our findings indicated the substantial antibacterial potential of AWME of <i>H. illucens</i> larvae fat against these actual phytopathogens, thus paving the way for further research to determine the mechanism of action in crop protection.https://www.mdpi.com/2076-2607/8/9/1423black soldier flyfatty acidslarvae extractphytopathogenic bacteriaantibacterial activity |
| spellingShingle | Elena Marusich Heakal Mohamed Yuriy Afanasev Sergey Leonov Fatty Acids from <i>Hermetia illucens</i> Larvae Fat Inhibit the Proliferation and Growth of Actual Phytopathogens Microorganisms black soldier fly fatty acids larvae extract phytopathogenic bacteria antibacterial activity |
| title | Fatty Acids from <i>Hermetia illucens</i> Larvae Fat Inhibit the Proliferation and Growth of Actual Phytopathogens |
| title_full | Fatty Acids from <i>Hermetia illucens</i> Larvae Fat Inhibit the Proliferation and Growth of Actual Phytopathogens |
| title_fullStr | Fatty Acids from <i>Hermetia illucens</i> Larvae Fat Inhibit the Proliferation and Growth of Actual Phytopathogens |
| title_full_unstemmed | Fatty Acids from <i>Hermetia illucens</i> Larvae Fat Inhibit the Proliferation and Growth of Actual Phytopathogens |
| title_short | Fatty Acids from <i>Hermetia illucens</i> Larvae Fat Inhibit the Proliferation and Growth of Actual Phytopathogens |
| title_sort | fatty acids from i hermetia illucens i larvae fat inhibit the proliferation and growth of actual phytopathogens |
| topic | black soldier fly fatty acids larvae extract phytopathogenic bacteria antibacterial activity |
| url | https://www.mdpi.com/2076-2607/8/9/1423 |
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