Raman Spectroscopy as a Robust New Tool for Rapid and Accurate Evaluation of Drought Tolerance Levels in Both Genetically Diverse and Near-Isogenic Maize Lines
Improving drought tolerance of crops has become crucial due to the current scenario of rapid climate change. In particular, development of new maize germplasm with increased drought tolerance is viewed as a major breeding goal to ensure sustainable food and feed production. Therefore, accurate rapid...
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.621711/full |
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| author | Narangerel Altangerel Narangerel Altangerel Pei-Cheng Huang Michael V. Kolomiets Marlan O. Scully Marlan O. Scully Marlan O. Scully Philip R. Hemmer |
| author_facet | Narangerel Altangerel Narangerel Altangerel Pei-Cheng Huang Michael V. Kolomiets Marlan O. Scully Marlan O. Scully Marlan O. Scully Philip R. Hemmer |
| author_sort | Narangerel Altangerel |
| collection | DOAJ |
| description | Improving drought tolerance of crops has become crucial due to the current scenario of rapid climate change. In particular, development of new maize germplasm with increased drought tolerance is viewed as a major breeding goal to ensure sustainable food and feed production. Therefore, accurate rapid phenotyping techniques for selection of superior maize genotypes are required. The objectives of this study were to determine whether Raman microscopy technique can be applied for accurate assessment of drought-tolerance levels in both genetically diverse and near-isogenic maize lines that differ in their levels of drought-tolerance. Carotenoid degradation is known to be a direct stress response initiated by reactive oxygen species during osmotic stress such as drought. Using Raman mapping, we observed real-time changes in the rate of carotenoid degradation in chloroplasts that was dependent on the strength of osmotic stress. In addition, we showed that the rate of carotenoid degradation as measured by Raman spectroscopy correlates directly with drought tolerance levels of diverse maize genotypes. We conclude that Raman technique is a robust, biochemically selective and non-invasive phenotyping technique that accurately distinguishes drought tolerance levels in both genetically diverse and near-isogenic maize genotypes. We conclude that this technique can be further developed to render it suitable for field-based early assessment of breeding materials with superior drought-tolerance traits. |
| first_indexed | 2024-12-19T09:14:56Z |
| format | Article |
| id | doaj.art-7e5b789d0fa746789076d9a189f43054 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-19T09:14:56Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-7e5b789d0fa746789076d9a189f430542022-12-21T20:28:07ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-07-011210.3389/fpls.2021.621711621711Raman Spectroscopy as a Robust New Tool for Rapid and Accurate Evaluation of Drought Tolerance Levels in Both Genetically Diverse and Near-Isogenic Maize LinesNarangerel Altangerel0Narangerel Altangerel1Pei-Cheng Huang2Michael V. Kolomiets3Marlan O. Scully4Marlan O. Scully5Marlan O. Scully6Philip R. Hemmer7Institute of Quantum Science and Engineering, Texas A&M University, College Station, TX, United StatesPhysics Department, Baylor University, Waco, TX, United StatesPlant Pathology and Microbiology Department, Texas A&M University, College Station, TX, United StatesPlant Pathology and Microbiology Department, Texas A&M University, College Station, TX, United StatesInstitute of Quantum Science and Engineering, Texas A&M University, College Station, TX, United StatesPhysics Department, Baylor University, Waco, TX, United StatesMechanical and Aerospace Engineering, Princeton University, Princeton, NJ, United StatesElectrical and Computer Engineering Department, Texas A&M University, College Station, TX, United StatesImproving drought tolerance of crops has become crucial due to the current scenario of rapid climate change. In particular, development of new maize germplasm with increased drought tolerance is viewed as a major breeding goal to ensure sustainable food and feed production. Therefore, accurate rapid phenotyping techniques for selection of superior maize genotypes are required. The objectives of this study were to determine whether Raman microscopy technique can be applied for accurate assessment of drought-tolerance levels in both genetically diverse and near-isogenic maize lines that differ in their levels of drought-tolerance. Carotenoid degradation is known to be a direct stress response initiated by reactive oxygen species during osmotic stress such as drought. Using Raman mapping, we observed real-time changes in the rate of carotenoid degradation in chloroplasts that was dependent on the strength of osmotic stress. In addition, we showed that the rate of carotenoid degradation as measured by Raman spectroscopy correlates directly with drought tolerance levels of diverse maize genotypes. We conclude that Raman technique is a robust, biochemically selective and non-invasive phenotyping technique that accurately distinguishes drought tolerance levels in both genetically diverse and near-isogenic maize genotypes. We conclude that this technique can be further developed to render it suitable for field-based early assessment of breeding materials with superior drought-tolerance traits.https://www.frontiersin.org/articles/10.3389/fpls.2021.621711/fulllipoxygenaseRaman spectroscopycarotenoid degradationosmotic stressdrought stressmaize inbred lines |
| spellingShingle | Narangerel Altangerel Narangerel Altangerel Pei-Cheng Huang Michael V. Kolomiets Marlan O. Scully Marlan O. Scully Marlan O. Scully Philip R. Hemmer Raman Spectroscopy as a Robust New Tool for Rapid and Accurate Evaluation of Drought Tolerance Levels in Both Genetically Diverse and Near-Isogenic Maize Lines Frontiers in Plant Science lipoxygenase Raman spectroscopy carotenoid degradation osmotic stress drought stress maize inbred lines |
| title | Raman Spectroscopy as a Robust New Tool for Rapid and Accurate Evaluation of Drought Tolerance Levels in Both Genetically Diverse and Near-Isogenic Maize Lines |
| title_full | Raman Spectroscopy as a Robust New Tool for Rapid and Accurate Evaluation of Drought Tolerance Levels in Both Genetically Diverse and Near-Isogenic Maize Lines |
| title_fullStr | Raman Spectroscopy as a Robust New Tool for Rapid and Accurate Evaluation of Drought Tolerance Levels in Both Genetically Diverse and Near-Isogenic Maize Lines |
| title_full_unstemmed | Raman Spectroscopy as a Robust New Tool for Rapid and Accurate Evaluation of Drought Tolerance Levels in Both Genetically Diverse and Near-Isogenic Maize Lines |
| title_short | Raman Spectroscopy as a Robust New Tool for Rapid and Accurate Evaluation of Drought Tolerance Levels in Both Genetically Diverse and Near-Isogenic Maize Lines |
| title_sort | raman spectroscopy as a robust new tool for rapid and accurate evaluation of drought tolerance levels in both genetically diverse and near isogenic maize lines |
| topic | lipoxygenase Raman spectroscopy carotenoid degradation osmotic stress drought stress maize inbred lines |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.621711/full |
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