First Detection of the West Nile Virus Koutango Lineage in Sandflies in Niger
West Nile virus (WNV), belonging to the <i>Flaviviridae</i> family, causes a mosquito-borne disease and shows great genetic diversity, with at least eight different lineages. The Koutango lineage of WNV (WN-KOUTV), mostly associated with ticks and rodents in the wild, is exclusively pres...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/10/3/257 |
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| author | Gamou Fall Diawo Diallo Hadiza Soumaila El Hadji Ndiaye Adamou Lagare Bacary Djilocalisse Sadio Marie Henriette Dior Ndione Michael Wiley Moussa Dia Mamadou Diop Arame Ba Fati Sidikou Bienvenu Baruani Ngoy Oumar Faye Jean Testa Cheikh Loucoubar Amadou Alpha Sall Mawlouth Diallo Ousmane Faye |
| author_facet | Gamou Fall Diawo Diallo Hadiza Soumaila El Hadji Ndiaye Adamou Lagare Bacary Djilocalisse Sadio Marie Henriette Dior Ndione Michael Wiley Moussa Dia Mamadou Diop Arame Ba Fati Sidikou Bienvenu Baruani Ngoy Oumar Faye Jean Testa Cheikh Loucoubar Amadou Alpha Sall Mawlouth Diallo Ousmane Faye |
| author_sort | Gamou Fall |
| collection | DOAJ |
| description | West Nile virus (WNV), belonging to the <i>Flaviviridae</i> family, causes a mosquito-borne disease and shows great genetic diversity, with at least eight different lineages. The Koutango lineage of WNV (WN-KOUTV), mostly associated with ticks and rodents in the wild, is exclusively present in Africa and shows evidence of infection in humans and high virulence in mice. In 2016, in a context of Rift Valley fever (RVF) outbreak in Niger, mosquitoes, biting midges and sandflies were collected for arbovirus isolation using cell culture, immunofluorescence and RT-PCR assays. Whole genome sequencing and in vivo replication studies using mice were later conducted on positive samples. The WN-KOUTV strain was detected in a sandfly pool. The sequence analyses and replication studies confirmed that this strain belonged to the WN-KOUTV lineage and caused 100% mortality of mice. Further studies should be done to assess what genetic traits of WN-KOUTV influence this very high virulence in mice. In addition, given the risk of WN-KOUTV to infect humans, the possibility of multiple vectors as well as birds as reservoirs of WNV, to spread the virus beyond Africa, and the increasing threats of flavivirus infections in the world, it is important to understand the potential of WN-KOUTV to emerge. |
| first_indexed | 2024-03-09T00:33:32Z |
| format | Article |
| id | doaj.art-7e6077af364b451ab974e34c33995a49 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T00:33:32Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-7e6077af364b451ab974e34c33995a492023-12-11T18:18:27ZengMDPI AGPathogens2076-08172021-02-0110325710.3390/pathogens10030257First Detection of the West Nile Virus Koutango Lineage in Sandflies in NigerGamou Fall0Diawo Diallo1Hadiza Soumaila2El Hadji Ndiaye3Adamou Lagare4Bacary Djilocalisse Sadio5Marie Henriette Dior Ndione6Michael Wiley7Moussa Dia8Mamadou Diop9Arame Ba10Fati Sidikou11Bienvenu Baruani Ngoy12Oumar Faye13Jean Testa14Cheikh Loucoubar15Amadou Alpha Sall16Mawlouth Diallo17Ousmane Faye18Pole of Virology, WHO Collaborating Center For Arbovirus and Haemorrhagic Fever Virus, Institut Pasteur, Dakar BP 220, SenegalPole of Zoology, Medical Entomology Unit, Institut Pasteur, Dakar BP 220, SenegalProgramme National de Lutte contre le Paludisme, Ministère de la Santé Publique du Niger, Niamey BP 623, NigerPole of Zoology, Medical Entomology Unit, Institut Pasteur, Dakar BP 220, SenegalCentre de Recherche Médicale et Sanitaire, Niamey BP 10887, NigerPole of Virology, WHO Collaborating Center For Arbovirus and Haemorrhagic Fever Virus, Institut Pasteur, Dakar BP 220, SenegalPole of Virology, WHO Collaborating Center For Arbovirus and Haemorrhagic Fever Virus, Institut Pasteur, Dakar BP 220, SenegalUnited States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702-5011, USAPole of Virology, WHO Collaborating Center For Arbovirus and Haemorrhagic Fever Virus, Institut Pasteur, Dakar BP 220, SenegalBiostatistic, Biomathematics and Modelling Group, Institut Pasteur, Dakar BP 220, SenegalPole of Virology, WHO Collaborating Center For Arbovirus and Haemorrhagic Fever Virus, Institut Pasteur, Dakar BP 220, SenegalCentre de Recherche Médicale et Sanitaire, Niamey BP 10887, NigerWHO Country Office, Niamey B.P. 10739, NigerPole of Virology, WHO Collaborating Center For Arbovirus and Haemorrhagic Fever Virus, Institut Pasteur, Dakar BP 220, SenegalCentre de Recherche Médicale et Sanitaire, Niamey BP 10887, NigerBiostatistic, Biomathematics and Modelling Group, Institut Pasteur, Dakar BP 220, SenegalPole of Virology, WHO Collaborating Center For Arbovirus and Haemorrhagic Fever Virus, Institut Pasteur, Dakar BP 220, SenegalPole of Zoology, Medical Entomology Unit, Institut Pasteur, Dakar BP 220, SenegalPole of Virology, WHO Collaborating Center For Arbovirus and Haemorrhagic Fever Virus, Institut Pasteur, Dakar BP 220, SenegalWest Nile virus (WNV), belonging to the <i>Flaviviridae</i> family, causes a mosquito-borne disease and shows great genetic diversity, with at least eight different lineages. The Koutango lineage of WNV (WN-KOUTV), mostly associated with ticks and rodents in the wild, is exclusively present in Africa and shows evidence of infection in humans and high virulence in mice. In 2016, in a context of Rift Valley fever (RVF) outbreak in Niger, mosquitoes, biting midges and sandflies were collected for arbovirus isolation using cell culture, immunofluorescence and RT-PCR assays. Whole genome sequencing and in vivo replication studies using mice were later conducted on positive samples. The WN-KOUTV strain was detected in a sandfly pool. The sequence analyses and replication studies confirmed that this strain belonged to the WN-KOUTV lineage and caused 100% mortality of mice. Further studies should be done to assess what genetic traits of WN-KOUTV influence this very high virulence in mice. In addition, given the risk of WN-KOUTV to infect humans, the possibility of multiple vectors as well as birds as reservoirs of WNV, to spread the virus beyond Africa, and the increasing threats of flavivirus infections in the world, it is important to understand the potential of WN-KOUTV to emerge.https://www.mdpi.com/2076-0817/10/3/257West Nile virusKoutango lineagehigh virulencesandfliesNiger |
| spellingShingle | Gamou Fall Diawo Diallo Hadiza Soumaila El Hadji Ndiaye Adamou Lagare Bacary Djilocalisse Sadio Marie Henriette Dior Ndione Michael Wiley Moussa Dia Mamadou Diop Arame Ba Fati Sidikou Bienvenu Baruani Ngoy Oumar Faye Jean Testa Cheikh Loucoubar Amadou Alpha Sall Mawlouth Diallo Ousmane Faye First Detection of the West Nile Virus Koutango Lineage in Sandflies in Niger Pathogens West Nile virus Koutango lineage high virulence sandflies Niger |
| title | First Detection of the West Nile Virus Koutango Lineage in Sandflies in Niger |
| title_full | First Detection of the West Nile Virus Koutango Lineage in Sandflies in Niger |
| title_fullStr | First Detection of the West Nile Virus Koutango Lineage in Sandflies in Niger |
| title_full_unstemmed | First Detection of the West Nile Virus Koutango Lineage in Sandflies in Niger |
| title_short | First Detection of the West Nile Virus Koutango Lineage in Sandflies in Niger |
| title_sort | first detection of the west nile virus koutango lineage in sandflies in niger |
| topic | West Nile virus Koutango lineage high virulence sandflies Niger |
| url | https://www.mdpi.com/2076-0817/10/3/257 |
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