Whole genome sequencing analyses revealed that Salmonella enterica serovar Dublin strains from Brazil belonged to two predominant clades
Abstract Salmonella Dublin is a cattle-associated serovar sporadically causing disease in humans. S. Dublin strains isolated in Brazil and in other countries were analyzed to determine their phylogenetic relationships, the presence of genes, plasmids, genomic regions related to virulence and antimic...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-14492-4 |
| _version_ | 1811230776618909696 |
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| author | Fábio Campioni Felipe Pinheiro Vilela Guojie Cao George Kastanis Dália dos Prazeres Rodrigues Renata Garcia Costa Monique Ribeiro Tiba-Casas Lanlan Yin Marc Allard Juliana Pfrimer Falcão |
| author_facet | Fábio Campioni Felipe Pinheiro Vilela Guojie Cao George Kastanis Dália dos Prazeres Rodrigues Renata Garcia Costa Monique Ribeiro Tiba-Casas Lanlan Yin Marc Allard Juliana Pfrimer Falcão |
| author_sort | Fábio Campioni |
| collection | DOAJ |
| description | Abstract Salmonella Dublin is a cattle-associated serovar sporadically causing disease in humans. S. Dublin strains isolated in Brazil and in other countries were analyzed to determine their phylogenetic relationships, the presence of genes, plasmids, genomic regions related to virulence and antimicrobial resistance genes repertoire, using WGS analyses. Illumina was used to sequence the genome of 112 S. Dublin strains isolated in Brazil from humans (n = 82) and animals (n = 30) between 1983 and 2016. Furthermore, 87 strains from other countries were analyzed. WGSNP analysis revealed three different clades, in which the strains from Brazil belonged to two clades, A and C. Most of the genes and genomic regions searched varied among the strains studied. The siderophore genes iroB and iroC were exclusively found in strains from Brazil and pegD gene, related to fimbrial adherence determinants, were positive in 124 strains from clades A and B but absent in all the strains from clade C (n = 71). Eleven plasmid replicons were found in the strains from Brazil, and nine were exclusively found in strains from other countries. The antimicrobial resistance genes mdsA and mdsB, that encode an efflux pump, were found in all the strains studied. The strains from Brazil carried other resistance genes, such as tet(A) (n = 11), tet(B) (n = 4) and tet(C) (n = 4), blaTEM-1 (n = 4), catA1 (n = 1), aadA1 (n = 1), and sul1 (n = 1). In conclusion, S. Dublin strains isolated in Brazil presented some few unique genes not found in strains from other countries and were allocated into two distinct clades with strains of human and animal origin epidemiologically related. This fact stresses the zoonotic potential of S. Dublin circulating in Brazil for more than 30 years. |
| first_indexed | 2024-04-12T10:33:45Z |
| format | Article |
| id | doaj.art-7e624b9add2c41258ae0eb74424788f4 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T10:33:45Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-7e624b9add2c41258ae0eb74424788f42022-12-22T03:36:46ZengNature PortfolioScientific Reports2045-23222022-06-011211610.1038/s41598-022-14492-4Whole genome sequencing analyses revealed that Salmonella enterica serovar Dublin strains from Brazil belonged to two predominant cladesFábio Campioni0Felipe Pinheiro Vilela1Guojie Cao2George Kastanis3Dália dos Prazeres Rodrigues4Renata Garcia Costa5Monique Ribeiro Tiba-Casas6Lanlan Yin7Marc Allard8Juliana Pfrimer Falcão9Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto - USPDepartamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto - USPDivision of Microbiology, Office of Regulatory Science, Center for Food Safety and Applied Nutrition, U.S. Food and Drug AdministrationDivision of Microbiology, Office of Regulatory Science, Center for Food Safety and Applied Nutrition, U.S. Food and Drug AdministrationLaboratório de Enterobactérias, FIOCRUZ/Fundação Instituto Oswaldo CruzLaboratório de Enterobactérias, FIOCRUZ/Fundação Instituto Oswaldo CruzCentro de Bacteriologia, Instituto Adolfo LutzOffice of Analytics and Outreach, Center for Food Safety and Applied Nutrition, U.S. Food and Drug AdministrationDivision of Microbiology, Office of Regulatory Science, Center for Food Safety and Applied Nutrition, U.S. Food and Drug AdministrationDepartamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto - USPAbstract Salmonella Dublin is a cattle-associated serovar sporadically causing disease in humans. S. Dublin strains isolated in Brazil and in other countries were analyzed to determine their phylogenetic relationships, the presence of genes, plasmids, genomic regions related to virulence and antimicrobial resistance genes repertoire, using WGS analyses. Illumina was used to sequence the genome of 112 S. Dublin strains isolated in Brazil from humans (n = 82) and animals (n = 30) between 1983 and 2016. Furthermore, 87 strains from other countries were analyzed. WGSNP analysis revealed three different clades, in which the strains from Brazil belonged to two clades, A and C. Most of the genes and genomic regions searched varied among the strains studied. The siderophore genes iroB and iroC were exclusively found in strains from Brazil and pegD gene, related to fimbrial adherence determinants, were positive in 124 strains from clades A and B but absent in all the strains from clade C (n = 71). Eleven plasmid replicons were found in the strains from Brazil, and nine were exclusively found in strains from other countries. The antimicrobial resistance genes mdsA and mdsB, that encode an efflux pump, were found in all the strains studied. The strains from Brazil carried other resistance genes, such as tet(A) (n = 11), tet(B) (n = 4) and tet(C) (n = 4), blaTEM-1 (n = 4), catA1 (n = 1), aadA1 (n = 1), and sul1 (n = 1). In conclusion, S. Dublin strains isolated in Brazil presented some few unique genes not found in strains from other countries and were allocated into two distinct clades with strains of human and animal origin epidemiologically related. This fact stresses the zoonotic potential of S. Dublin circulating in Brazil for more than 30 years.https://doi.org/10.1038/s41598-022-14492-4 |
| spellingShingle | Fábio Campioni Felipe Pinheiro Vilela Guojie Cao George Kastanis Dália dos Prazeres Rodrigues Renata Garcia Costa Monique Ribeiro Tiba-Casas Lanlan Yin Marc Allard Juliana Pfrimer Falcão Whole genome sequencing analyses revealed that Salmonella enterica serovar Dublin strains from Brazil belonged to two predominant clades Scientific Reports |
| title | Whole genome sequencing analyses revealed that Salmonella enterica serovar Dublin strains from Brazil belonged to two predominant clades |
| title_full | Whole genome sequencing analyses revealed that Salmonella enterica serovar Dublin strains from Brazil belonged to two predominant clades |
| title_fullStr | Whole genome sequencing analyses revealed that Salmonella enterica serovar Dublin strains from Brazil belonged to two predominant clades |
| title_full_unstemmed | Whole genome sequencing analyses revealed that Salmonella enterica serovar Dublin strains from Brazil belonged to two predominant clades |
| title_short | Whole genome sequencing analyses revealed that Salmonella enterica serovar Dublin strains from Brazil belonged to two predominant clades |
| title_sort | whole genome sequencing analyses revealed that salmonella enterica serovar dublin strains from brazil belonged to two predominant clades |
| url | https://doi.org/10.1038/s41598-022-14492-4 |
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