Comparative analysis of physiological variations and genetic architecture for cold stress response in soybean germplasm
Soybean (Glycine max L.) is susceptible to low temperatures. Increasing lines of evidence indicate that abiotic stress-responsive genes are involved in plant low-temperature stress response. However, the involvement of photosynthesis, antioxidants and metabolites genes in low temperature response is...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-01-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1095335/full |
| _version_ | 1797959908637802496 |
|---|---|
| author | Muhammad Azhar Hussain Muhammad Azhar Hussain Senquan Li Hongtao Gao Hongtao Gao Chen Feng Pengyu Sun Xiangpeng Sui Yan Jing Yan Jing Keheng Xu Keheng Xu Yonggang Zhou Yonggang Zhou Wenping Zhang Wenping Zhang Haiyan Li Haiyan Li |
| author_facet | Muhammad Azhar Hussain Muhammad Azhar Hussain Senquan Li Hongtao Gao Hongtao Gao Chen Feng Pengyu Sun Xiangpeng Sui Yan Jing Yan Jing Keheng Xu Keheng Xu Yonggang Zhou Yonggang Zhou Wenping Zhang Wenping Zhang Haiyan Li Haiyan Li |
| author_sort | Muhammad Azhar Hussain |
| collection | DOAJ |
| description | Soybean (Glycine max L.) is susceptible to low temperatures. Increasing lines of evidence indicate that abiotic stress-responsive genes are involved in plant low-temperature stress response. However, the involvement of photosynthesis, antioxidants and metabolites genes in low temperature response is largely unexplored in Soybean. In the current study, a genetic panel of diverse soybean varieties was analyzed for photosynthesis, chlorophyll fluorescence and leaf injury parameters under cold stress and control conditions. This helps us to identify cold tolerant (V100) and cold sensitive (V45) varieties. The V100 variety outperformed for antioxidant enzymes activities and relative expression of photosynthesis (Glyma.08G204800.1, Glyma.12G232000.1), GmSOD (GmSOD01, GmSOD08), GmPOD (GmPOD29, GmPOD47), trehalose (GmTPS01, GmTPS13) and cold marker genes (DREB1E, DREB1D, SCOF1) than V45 under cold stress. Upon cold stress, the V100 variety showed reduced accumulation of H2O2 and MDA levels and subsequently showed lower leaf injury compared to V45. Together, our results uncovered new avenues for identifying cold tolerant soybean varieties from a large panel. Additionally, we identified the role of antioxidants, osmo-protectants and their posttranscriptional regulators miRNAs such as miR319, miR394, miR397, and miR398 in Soybean cold stress tolerance. |
| first_indexed | 2024-04-11T00:38:11Z |
| format | Article |
| id | doaj.art-7e653261af404477acf8aef8e295e72e |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T00:38:11Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-7e653261af404477acf8aef8e295e72e2023-01-06T13:31:31ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-01-011310.3389/fpls.2022.10953351095335Comparative analysis of physiological variations and genetic architecture for cold stress response in soybean germplasmMuhammad Azhar Hussain0Muhammad Azhar Hussain1Senquan Li2Hongtao Gao3Hongtao Gao4Chen Feng5Pengyu Sun6Xiangpeng Sui7Yan Jing8Yan Jing9Keheng Xu10Keheng Xu11Yonggang Zhou12Yonggang Zhou13Wenping Zhang14Wenping Zhang15Haiyan Li16Haiyan Li17Sanya Nanfan Research Institute of Hainan University, Hainan Yazhou Bay Seed Laboratory, Sanya, ChinaCollege of Tropical Crops, Hainan University, Haikou, ChinaCollege of Tropical Crops, Hainan University, Haikou, ChinaSanya Nanfan Research Institute of Hainan University, Hainan Yazhou Bay Seed Laboratory, Sanya, ChinaCollege of Tropical Crops, Hainan University, Haikou, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun, ChinaCollege of Tropical Crops, Hainan University, Haikou, ChinaCollege of Tropical Crops, Hainan University, Haikou, ChinaSanya Nanfan Research Institute of Hainan University, Hainan Yazhou Bay Seed Laboratory, Sanya, ChinaCollege of Tropical Crops, Hainan University, Haikou, ChinaSanya Nanfan Research Institute of Hainan University, Hainan Yazhou Bay Seed Laboratory, Sanya, ChinaCollege of Tropical Crops, Hainan University, Haikou, ChinaSanya Nanfan Research Institute of Hainan University, Hainan Yazhou Bay Seed Laboratory, Sanya, ChinaCollege of Tropical Crops, Hainan University, Haikou, ChinaSanya Nanfan Research Institute of Hainan University, Hainan Yazhou Bay Seed Laboratory, Sanya, ChinaCollege of Tropical Crops, Hainan University, Haikou, ChinaSanya Nanfan Research Institute of Hainan University, Hainan Yazhou Bay Seed Laboratory, Sanya, ChinaCollege of Tropical Crops, Hainan University, Haikou, ChinaSoybean (Glycine max L.) is susceptible to low temperatures. Increasing lines of evidence indicate that abiotic stress-responsive genes are involved in plant low-temperature stress response. However, the involvement of photosynthesis, antioxidants and metabolites genes in low temperature response is largely unexplored in Soybean. In the current study, a genetic panel of diverse soybean varieties was analyzed for photosynthesis, chlorophyll fluorescence and leaf injury parameters under cold stress and control conditions. This helps us to identify cold tolerant (V100) and cold sensitive (V45) varieties. The V100 variety outperformed for antioxidant enzymes activities and relative expression of photosynthesis (Glyma.08G204800.1, Glyma.12G232000.1), GmSOD (GmSOD01, GmSOD08), GmPOD (GmPOD29, GmPOD47), trehalose (GmTPS01, GmTPS13) and cold marker genes (DREB1E, DREB1D, SCOF1) than V45 under cold stress. Upon cold stress, the V100 variety showed reduced accumulation of H2O2 and MDA levels and subsequently showed lower leaf injury compared to V45. Together, our results uncovered new avenues for identifying cold tolerant soybean varieties from a large panel. Additionally, we identified the role of antioxidants, osmo-protectants and their posttranscriptional regulators miRNAs such as miR319, miR394, miR397, and miR398 in Soybean cold stress tolerance.https://www.frontiersin.org/articles/10.3389/fpls.2022.1095335/fullcold stressRNA-seqmiRNAphotosynthesisantioxidantssoybean |
| spellingShingle | Muhammad Azhar Hussain Muhammad Azhar Hussain Senquan Li Hongtao Gao Hongtao Gao Chen Feng Pengyu Sun Xiangpeng Sui Yan Jing Yan Jing Keheng Xu Keheng Xu Yonggang Zhou Yonggang Zhou Wenping Zhang Wenping Zhang Haiyan Li Haiyan Li Comparative analysis of physiological variations and genetic architecture for cold stress response in soybean germplasm Frontiers in Plant Science cold stress RNA-seq miRNA photosynthesis antioxidants soybean |
| title | Comparative analysis of physiological variations and genetic architecture for cold stress response in soybean germplasm |
| title_full | Comparative analysis of physiological variations and genetic architecture for cold stress response in soybean germplasm |
| title_fullStr | Comparative analysis of physiological variations and genetic architecture for cold stress response in soybean germplasm |
| title_full_unstemmed | Comparative analysis of physiological variations and genetic architecture for cold stress response in soybean germplasm |
| title_short | Comparative analysis of physiological variations and genetic architecture for cold stress response in soybean germplasm |
| title_sort | comparative analysis of physiological variations and genetic architecture for cold stress response in soybean germplasm |
| topic | cold stress RNA-seq miRNA photosynthesis antioxidants soybean |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1095335/full |
| work_keys_str_mv | AT muhammadazharhussain comparativeanalysisofphysiologicalvariationsandgeneticarchitectureforcoldstressresponseinsoybeangermplasm AT muhammadazharhussain comparativeanalysisofphysiologicalvariationsandgeneticarchitectureforcoldstressresponseinsoybeangermplasm AT senquanli comparativeanalysisofphysiologicalvariationsandgeneticarchitectureforcoldstressresponseinsoybeangermplasm AT hongtaogao comparativeanalysisofphysiologicalvariationsandgeneticarchitectureforcoldstressresponseinsoybeangermplasm AT hongtaogao comparativeanalysisofphysiologicalvariationsandgeneticarchitectureforcoldstressresponseinsoybeangermplasm AT chenfeng comparativeanalysisofphysiologicalvariationsandgeneticarchitectureforcoldstressresponseinsoybeangermplasm AT pengyusun comparativeanalysisofphysiologicalvariationsandgeneticarchitectureforcoldstressresponseinsoybeangermplasm AT xiangpengsui comparativeanalysisofphysiologicalvariationsandgeneticarchitectureforcoldstressresponseinsoybeangermplasm AT yanjing comparativeanalysisofphysiologicalvariationsandgeneticarchitectureforcoldstressresponseinsoybeangermplasm AT yanjing comparativeanalysisofphysiologicalvariationsandgeneticarchitectureforcoldstressresponseinsoybeangermplasm AT kehengxu comparativeanalysisofphysiologicalvariationsandgeneticarchitectureforcoldstressresponseinsoybeangermplasm AT kehengxu comparativeanalysisofphysiologicalvariationsandgeneticarchitectureforcoldstressresponseinsoybeangermplasm AT yonggangzhou comparativeanalysisofphysiologicalvariationsandgeneticarchitectureforcoldstressresponseinsoybeangermplasm AT yonggangzhou comparativeanalysisofphysiologicalvariationsandgeneticarchitectureforcoldstressresponseinsoybeangermplasm AT wenpingzhang comparativeanalysisofphysiologicalvariationsandgeneticarchitectureforcoldstressresponseinsoybeangermplasm AT wenpingzhang comparativeanalysisofphysiologicalvariationsandgeneticarchitectureforcoldstressresponseinsoybeangermplasm AT haiyanli comparativeanalysisofphysiologicalvariationsandgeneticarchitectureforcoldstressresponseinsoybeangermplasm AT haiyanli comparativeanalysisofphysiologicalvariationsandgeneticarchitectureforcoldstressresponseinsoybeangermplasm |