Characterization and Antimicrobial-Resistance Profile of Escherichia coli O157 and O157: H7 Isolated from Modified Atmosphere Packaged Meat Samples
Shiga-like toxin producing Escherichia coli is still an important public issue which causes extremely dangerous health problems. This study was planned in order to examine the inhibitory effect of Modified Atmosphere Packaging application on E. coli O157 and O157: H7. The purposes of the present stu...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Turkish Science and Technology Publishing (TURSTEP)
2017-10-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
| Subjects: | |
| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/1228 |
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| author | Özgür Çadırcı Ali Gücükoğlu Göknur Terzi Güzel Tolga Uyanık Abdulaziz Abdulahi Mustafa Alişarlı |
| author_facet | Özgür Çadırcı Ali Gücükoğlu Göknur Terzi Güzel Tolga Uyanık Abdulaziz Abdulahi Mustafa Alişarlı |
| author_sort | Özgür Çadırcı |
| collection | DOAJ |
| description | Shiga-like toxin producing Escherichia coli is still an important public issue which causes extremely dangerous health problems. This study was planned in order to examine the inhibitory effect of Modified Atmosphere Packaging application on E. coli O157 and O157: H7. The purposes of the present study were to detect E. coli O157 and O157: H7 strains from ground and cubed beef. A total of 100 MAP cattle meat products (50 minced meat, 50 meat cubes) were collected from the markets and butchers in Samsun province between May and October 2013. According to results, 1(1/50-2%) E. coli O157 and 1(1/50-2%) E. coli O157: H7 strains isolated from 50 ground beef samples, while 1 (1/50-2%) E. coli O157 strain was identified from 50 cubed beef samples. It was determined that E. coli O157 isolate obtained from the MAP ground beef carried stx1, stx2 genes; E. coli O157: H7 isolate carried stx1, stx2, eaeA and hylA genes while E. coli O157 isolate obtained from the MAP cubed meat only carried the stx2 gene. In antibiogram test, both E. coli O157 isolates were resistant to streptomycin and one E. coli O157: H7 isolate was resistant to streptomycin, cephalothin and tetracycline. As a consequence; in order to protect public health, products should be kept in proper hygienic and technical conditions during sale and storage and use of uncontrolled antibiotics should be avoided. |
| first_indexed | 2024-04-10T14:30:39Z |
| format | Article |
| id | doaj.art-7e6719a082644acb99be91dceaaab29d |
| institution | Directory Open Access Journal |
| issn | 2148-127X |
| language | English |
| last_indexed | 2024-04-10T14:30:39Z |
| publishDate | 2017-10-01 |
| publisher | Turkish Science and Technology Publishing (TURSTEP) |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj.art-7e6719a082644acb99be91dceaaab29d2023-02-15T16:08:50ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2017-10-015101142114710.24925/turjaf.v5i10.1142-1147.1228620Characterization and Antimicrobial-Resistance Profile of Escherichia coli O157 and O157: H7 Isolated from Modified Atmosphere Packaged Meat SamplesÖzgür Çadırcı0Ali Gücükoğlu1Göknur Terzi Güzel2Tolga Uyanık3Abdulaziz Abdulahi4Mustafa Alişarlı5Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, University of Ondokuz Mayıs, 55200 SamsunDepartment of Food Hygiene and Technology, Faculty of Veterinary Medicine, University of Ondokuz Mayıs, 55200 SamsunDepartment of Food Hygiene and Technology, Faculty of Veterinary Medicine, University of Ondokuz Mayıs, 55200 SamsunDepartment of Food Hygiene and Technology, Faculty of Veterinary Medicine, University of Ondokuz Mayıs, 55200 SamsunDepartment of Food Hygiene and Technology, Faculty of Veterinary Medicine, University of Ondokuz Mayıs, 55200 SamsunOndokuz Mayıs Üniversitesi, Veteriner Fakültesi, Besin Hijyeni ve Teknolojisi BölümüShiga-like toxin producing Escherichia coli is still an important public issue which causes extremely dangerous health problems. This study was planned in order to examine the inhibitory effect of Modified Atmosphere Packaging application on E. coli O157 and O157: H7. The purposes of the present study were to detect E. coli O157 and O157: H7 strains from ground and cubed beef. A total of 100 MAP cattle meat products (50 minced meat, 50 meat cubes) were collected from the markets and butchers in Samsun province between May and October 2013. According to results, 1(1/50-2%) E. coli O157 and 1(1/50-2%) E. coli O157: H7 strains isolated from 50 ground beef samples, while 1 (1/50-2%) E. coli O157 strain was identified from 50 cubed beef samples. It was determined that E. coli O157 isolate obtained from the MAP ground beef carried stx1, stx2 genes; E. coli O157: H7 isolate carried stx1, stx2, eaeA and hylA genes while E. coli O157 isolate obtained from the MAP cubed meat only carried the stx2 gene. In antibiogram test, both E. coli O157 isolates were resistant to streptomycin and one E. coli O157: H7 isolate was resistant to streptomycin, cephalothin and tetracycline. As a consequence; in order to protect public health, products should be kept in proper hygienic and technical conditions during sale and storage and use of uncontrolled antibiotics should be avoided.http://www.agrifoodscience.com/index.php/TURJAF/article/view/1228MAP minced and cubed meatEscherichia coli O157:H7mPCRAntimicrobial resistance |
| spellingShingle | Özgür Çadırcı Ali Gücükoğlu Göknur Terzi Güzel Tolga Uyanık Abdulaziz Abdulahi Mustafa Alişarlı Characterization and Antimicrobial-Resistance Profile of Escherichia coli O157 and O157: H7 Isolated from Modified Atmosphere Packaged Meat Samples Turkish Journal of Agriculture: Food Science and Technology MAP minced and cubed meat Escherichia coli O157:H7 mPCR Antimicrobial resistance |
| title | Characterization and Antimicrobial-Resistance Profile of Escherichia coli O157 and O157: H7 Isolated from Modified Atmosphere Packaged Meat Samples |
| title_full | Characterization and Antimicrobial-Resistance Profile of Escherichia coli O157 and O157: H7 Isolated from Modified Atmosphere Packaged Meat Samples |
| title_fullStr | Characterization and Antimicrobial-Resistance Profile of Escherichia coli O157 and O157: H7 Isolated from Modified Atmosphere Packaged Meat Samples |
| title_full_unstemmed | Characterization and Antimicrobial-Resistance Profile of Escherichia coli O157 and O157: H7 Isolated from Modified Atmosphere Packaged Meat Samples |
| title_short | Characterization and Antimicrobial-Resistance Profile of Escherichia coli O157 and O157: H7 Isolated from Modified Atmosphere Packaged Meat Samples |
| title_sort | characterization and antimicrobial resistance profile of escherichia coli o157 and o157 h7 isolated from modified atmosphere packaged meat samples |
| topic | MAP minced and cubed meat Escherichia coli O157:H7 mPCR Antimicrobial resistance |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/1228 |
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