Evaluation of p-nitrobenzoic acid using Lowenstein–Jensen medium and rapid immunochromatographic test for early differentiation between Mycobacterium tuberculosis complex and non-tuberculous mycobacteria
Background: Mycobacterium tuberculosis (MTB), Mycobacterium bovis, Mycobacterium africanum, Mycobacterium canetti, and Mycobacterium microti are all members of the MTB complex [MTBC], which cause tuberculosis. Mycobacteria other than TB also known as non-tuberculous mycobacteria [NTM]. Aims and O...
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| Format: | Article |
| Language: | English |
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Manipal College of Medical Sciences, Pokhara
2022-08-01
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| Series: | Asian Journal of Medical Sciences |
| Subjects: | |
| Online Access: | https://www.nepjol.info/index.php/AJMS/article/view/43489 |
| _version_ | 1818176588104073216 |
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| author | Obulesu Gundala Praveen Kumar V Ramanesh Murthy Salma Mahaboob R |
| author_facet | Obulesu Gundala Praveen Kumar V Ramanesh Murthy Salma Mahaboob R |
| author_sort | Obulesu Gundala |
| collection | DOAJ |
| description | Background: Mycobacterium tuberculosis (MTB), Mycobacterium bovis, Mycobacterium africanum, Mycobacterium canetti, and Mycobacterium microti are all members of the MTB complex [MTBC], which cause tuberculosis. Mycobacteria other than TB also known as non-tuberculous mycobacteria [NTM].
Aims and Objectives: The purpose of this study was to see how accurate and efficient the MPT64 Ag fast test and Lowenstein–Jensen (LJ) medium with p-Nitrobenzoic acid (PNB) was in differentiating MTB and NTM.
Materials and Methods: This Prospective cross-sectional, diagnostic study was conducted in the department of Microbiology,
Chalamadaanandarao Medical college, Karimnagar, Telangana. India. The study was conducted from September 2020 to September 2021. The study was preapproved by the Institutional ethical and research committee.
Results: PNB inhibits the growth of MTBC, whereas NTM species were resistant. MPT64 is a protein antigen secreted specifically by the members of MTBC. This study was carried out to establish the accuracy and efficiency of LJ medium with PNB and MPT64 immunochromatography test (ICT) in differentiation between MTB and NTM. Of the 426 culture positive isolates tested, 382 (89.67%) were recovered from pulmonary specimens and 44 (10.32) from extrapulmonary sources. Of the 426 isolates tested for the presence of MPT64 antigen, 407 (95.53%) were found positive for ICT test. Whereas, 18 (4.22%) isolate and were considered as NTM and 408 as MTBC (95.77%) by selective inhibition by PNB in LJ medium.
Conclusion: According to our findings, the SDbioline kit is a fast, accurate, and reliable approach for separating MTB from NTM for TB management. MPT64ICT results were compared to culture smear results, which was particularly informative in the situation of MPT64ICT negative isolates with liquid cultures. |
| first_indexed | 2024-12-11T20:18:34Z |
| format | Article |
| id | doaj.art-7e6b3aa32efa430a86d2d343db885853 |
| institution | Directory Open Access Journal |
| issn | 2467-9100 2091-0576 |
| language | English |
| last_indexed | 2024-12-11T20:18:34Z |
| publishDate | 2022-08-01 |
| publisher | Manipal College of Medical Sciences, Pokhara |
| record_format | Article |
| series | Asian Journal of Medical Sciences |
| spelling | doaj.art-7e6b3aa32efa430a86d2d343db8858532022-12-22T00:52:08ZengManipal College of Medical Sciences, PokharaAsian Journal of Medical Sciences2467-91002091-05762022-08-01138145148https://doi.org/10.3126/ajms.v13i8.43489Evaluation of p-nitrobenzoic acid using Lowenstein–Jensen medium and rapid immunochromatographic test for early differentiation between Mycobacterium tuberculosis complex and non-tuberculous mycobacteriaObulesu Gundala 0https://orcid.org/0000-0002-4968-930XPraveen Kumar V 1https://orcid.org/0000-0002-7286-9907Ramanesh Murthy 2https://orcid.org/0000-0002-4071-8557Salma Mahaboob R 3https://orcid.org/0000-0002-5229-1159Associate Professor, Department of Microbiology, Rajmata Shrimati Devendra Kumari Singhdeo Government Medical College, Ambikapur, Chhattisgarh Associate Professor, Department of Microbiology, Chalamada Anand Rao Institute of Medical Sciences, Karimnagar, Telangana, India Dean, Department of Biochemistry, Rajmata Shrimati Devendra Kumari Singhdeo Government Medical College, Ambikapur, Chhattisgarh Associate Professor, Department of Biochemistry, Rajmata Shrimati Devendra Kumari Singhdeo Government Medical College, Ambikapur, Chhattisgarh Background: Mycobacterium tuberculosis (MTB), Mycobacterium bovis, Mycobacterium africanum, Mycobacterium canetti, and Mycobacterium microti are all members of the MTB complex [MTBC], which cause tuberculosis. Mycobacteria other than TB also known as non-tuberculous mycobacteria [NTM]. Aims and Objectives: The purpose of this study was to see how accurate and efficient the MPT64 Ag fast test and Lowenstein–Jensen (LJ) medium with p-Nitrobenzoic acid (PNB) was in differentiating MTB and NTM. Materials and Methods: This Prospective cross-sectional, diagnostic study was conducted in the department of Microbiology, Chalamadaanandarao Medical college, Karimnagar, Telangana. India. The study was conducted from September 2020 to September 2021. The study was preapproved by the Institutional ethical and research committee. Results: PNB inhibits the growth of MTBC, whereas NTM species were resistant. MPT64 is a protein antigen secreted specifically by the members of MTBC. This study was carried out to establish the accuracy and efficiency of LJ medium with PNB and MPT64 immunochromatography test (ICT) in differentiation between MTB and NTM. Of the 426 culture positive isolates tested, 382 (89.67%) were recovered from pulmonary specimens and 44 (10.32) from extrapulmonary sources. Of the 426 isolates tested for the presence of MPT64 antigen, 407 (95.53%) were found positive for ICT test. Whereas, 18 (4.22%) isolate and were considered as NTM and 408 as MTBC (95.77%) by selective inhibition by PNB in LJ medium. Conclusion: According to our findings, the SDbioline kit is a fast, accurate, and reliable approach for separating MTB from NTM for TB management. MPT64ICT results were compared to culture smear results, which was particularly informative in the situation of MPT64ICT negative isolates with liquid cultures.https://www.nepjol.info/index.php/AJMS/article/view/43489mpt 64 antigen; mycobacterium tuberculosis complex; non-tuberculous mycobacteria; para-nitrobenzoic acid |
| spellingShingle | Obulesu Gundala Praveen Kumar V Ramanesh Murthy Salma Mahaboob R Evaluation of p-nitrobenzoic acid using Lowenstein–Jensen medium and rapid immunochromatographic test for early differentiation between Mycobacterium tuberculosis complex and non-tuberculous mycobacteria Asian Journal of Medical Sciences mpt 64 antigen; mycobacterium tuberculosis complex; non-tuberculous mycobacteria; para-nitrobenzoic acid |
| title | Evaluation of p-nitrobenzoic acid using Lowenstein–Jensen medium and rapid immunochromatographic test for early differentiation between Mycobacterium tuberculosis complex and non-tuberculous mycobacteria |
| title_full | Evaluation of p-nitrobenzoic acid using Lowenstein–Jensen medium and rapid immunochromatographic test for early differentiation between Mycobacterium tuberculosis complex and non-tuberculous mycobacteria |
| title_fullStr | Evaluation of p-nitrobenzoic acid using Lowenstein–Jensen medium and rapid immunochromatographic test for early differentiation between Mycobacterium tuberculosis complex and non-tuberculous mycobacteria |
| title_full_unstemmed | Evaluation of p-nitrobenzoic acid using Lowenstein–Jensen medium and rapid immunochromatographic test for early differentiation between Mycobacterium tuberculosis complex and non-tuberculous mycobacteria |
| title_short | Evaluation of p-nitrobenzoic acid using Lowenstein–Jensen medium and rapid immunochromatographic test for early differentiation between Mycobacterium tuberculosis complex and non-tuberculous mycobacteria |
| title_sort | evaluation of p nitrobenzoic acid using lowenstein jensen medium and rapid immunochromatographic test for early differentiation between mycobacterium tuberculosis complex and non tuberculous mycobacteria |
| topic | mpt 64 antigen; mycobacterium tuberculosis complex; non-tuberculous mycobacteria; para-nitrobenzoic acid |
| url | https://www.nepjol.info/index.php/AJMS/article/view/43489 |
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