Identification of the source of blood meals in mosquitoes collected from north-eastern Australia
Abstract Background More than 70 arboviruses have been identified in Australia and the transmission cycles of most are poorly understood. While there is an extensive list of arthropods from which these viruses have been recovered, far less is known about the non-human hosts that may be involved in t...
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13071-019-3455-2 |
| _version_ | 1819089387543068672 |
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| author | Narayan Gyawali Andrew W. Taylor-Robinson Richard S. Bradbury David W. Huggins Leon E. Hugo Kym Lowry John G. Aaskov |
| author_facet | Narayan Gyawali Andrew W. Taylor-Robinson Richard S. Bradbury David W. Huggins Leon E. Hugo Kym Lowry John G. Aaskov |
| author_sort | Narayan Gyawali |
| collection | DOAJ |
| description | Abstract Background More than 70 arboviruses have been identified in Australia and the transmission cycles of most are poorly understood. While there is an extensive list of arthropods from which these viruses have been recovered, far less is known about the non-human hosts that may be involved in the transmission cycles of these viruses and the relative roles of different mosquito species in cycles of transmission involving different hosts. Some of the highest rates of human infection with zoonotic arboviruses, such as Ross River (RRV) and Barmah Forest (BFV) viruses, occur in coastal regions of north-eastern Australia. Methods Engorged mosquitoes collected as a part of routine surveillance using CO2-baited light traps in the Rockhampton Region and the adjoining Shire of Livingstone in central Queensland, north-eastern Australia, were analysed for the source of their blood meal. A 457 or 623 nucleotide region of the cytochrome b gene in the blood was amplified by PCR and the amplicons sequenced. The origin of the blood was identified by comparing the sequences obtained with those in GenBank®. Results The most common hosts for the mosquitoes sampled were domestic cattle (26/54) and wild birds (14/54). Humans (2/54) were an infrequent host for this range of mosquitoes that are known to transmit arboviruses causing human disease, and in an area where infections with human pathogens like RRV and BFV are commonly recorded. The blood meals identified in the most abundant vector analysed, Culex annulirostris, were from 10 different vertebrate hosts. The notable detection of chimpanzee blood in two mosquitoes, presumably obtained from a nearby zoo, extends the known range of hosts for this species. Culex quinquefasciatus and Cx. sitiens fed almost exclusively on a variety of bird species. Conclusions While human-mosquito-human transmission of arboviruses like RRV can occur, this study highlights the potential importance of zoonotic cycles of transmission, including avian species, of arboviruses that are indigenous to Australia. Further studies on larger samples of blood-engorged mosquitoes are required to validate the trends observed herein. Moreover, serological and virological evidence that the hosts on which the mosquitoes are feeding are being infected with arboviruses of interest are required. |
| first_indexed | 2024-12-21T22:07:08Z |
| format | Article |
| id | doaj.art-7e728ad4f1ae4cab9ca23823cd654dfd |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-21T22:07:08Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-7e728ad4f1ae4cab9ca23823cd654dfd2022-12-21T18:48:40ZengBMCParasites & Vectors1756-33052019-05-011211810.1186/s13071-019-3455-2Identification of the source of blood meals in mosquitoes collected from north-eastern AustraliaNarayan Gyawali0Andrew W. Taylor-Robinson1Richard S. Bradbury2David W. Huggins3Leon E. Hugo4Kym Lowry5John G. Aaskov6School of Health, Medical & Applied Sciences, Central Queensland UniversitySchool of Health, Medical & Applied Sciences, Central Queensland UniversitySchool of Health, Medical & Applied Sciences, Central Queensland UniversityPublic Environments, Livingstone Shire CouncilMosquito Control Laboratory, QIMR Berghofer Medical Research InstituteInstitute of Health & Biomedical Innovation, Queensland University of TechnologyInstitute of Health & Biomedical Innovation, Queensland University of TechnologyAbstract Background More than 70 arboviruses have been identified in Australia and the transmission cycles of most are poorly understood. While there is an extensive list of arthropods from which these viruses have been recovered, far less is known about the non-human hosts that may be involved in the transmission cycles of these viruses and the relative roles of different mosquito species in cycles of transmission involving different hosts. Some of the highest rates of human infection with zoonotic arboviruses, such as Ross River (RRV) and Barmah Forest (BFV) viruses, occur in coastal regions of north-eastern Australia. Methods Engorged mosquitoes collected as a part of routine surveillance using CO2-baited light traps in the Rockhampton Region and the adjoining Shire of Livingstone in central Queensland, north-eastern Australia, were analysed for the source of their blood meal. A 457 or 623 nucleotide region of the cytochrome b gene in the blood was amplified by PCR and the amplicons sequenced. The origin of the blood was identified by comparing the sequences obtained with those in GenBank®. Results The most common hosts for the mosquitoes sampled were domestic cattle (26/54) and wild birds (14/54). Humans (2/54) were an infrequent host for this range of mosquitoes that are known to transmit arboviruses causing human disease, and in an area where infections with human pathogens like RRV and BFV are commonly recorded. The blood meals identified in the most abundant vector analysed, Culex annulirostris, were from 10 different vertebrate hosts. The notable detection of chimpanzee blood in two mosquitoes, presumably obtained from a nearby zoo, extends the known range of hosts for this species. Culex quinquefasciatus and Cx. sitiens fed almost exclusively on a variety of bird species. Conclusions While human-mosquito-human transmission of arboviruses like RRV can occur, this study highlights the potential importance of zoonotic cycles of transmission, including avian species, of arboviruses that are indigenous to Australia. Further studies on larger samples of blood-engorged mosquitoes are required to validate the trends observed herein. Moreover, serological and virological evidence that the hosts on which the mosquitoes are feeding are being infected with arboviruses of interest are required.http://link.springer.com/article/10.1186/s13071-019-3455-2ArbovirusMosquitoCulexBlood mealHostTransmission |
| spellingShingle | Narayan Gyawali Andrew W. Taylor-Robinson Richard S. Bradbury David W. Huggins Leon E. Hugo Kym Lowry John G. Aaskov Identification of the source of blood meals in mosquitoes collected from north-eastern Australia Parasites & Vectors Arbovirus Mosquito Culex Blood meal Host Transmission |
| title | Identification of the source of blood meals in mosquitoes collected from north-eastern Australia |
| title_full | Identification of the source of blood meals in mosquitoes collected from north-eastern Australia |
| title_fullStr | Identification of the source of blood meals in mosquitoes collected from north-eastern Australia |
| title_full_unstemmed | Identification of the source of blood meals in mosquitoes collected from north-eastern Australia |
| title_short | Identification of the source of blood meals in mosquitoes collected from north-eastern Australia |
| title_sort | identification of the source of blood meals in mosquitoes collected from north eastern australia |
| topic | Arbovirus Mosquito Culex Blood meal Host Transmission |
| url | http://link.springer.com/article/10.1186/s13071-019-3455-2 |
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